106 research outputs found
Improvement of antibiotic activity of Xenorhabdus bovienii by medium optimization using response surface methodology
<p>Abstract</p> <p>Background</p> <p>The production of secondary metabolites with antibiotic properties is a common characteristic to entomopathogenic bacteria <it>Xenorhabdus</it> spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic, antimycotic and insecticidal, nematicidal and antiulcer, antineoplastic and antiviral. It has been known that cultivation parameters are critical to the secondary metabolites produced by microorganisms. Even small changes in the culture medium may not only impact the quantity of certain compounds but also the general metabolic profile of microorganisms. Manipulating nutritional or environmental factors can promote the biosynthesis of secondary metabolites and thus facilitate the discovery of new natural products. This work was conducted to evaluate the influence of nutrition on the antibiotic production of <it>X. bovienii</it> YL002 and to optimize the medium to maximize its antibiotic production.</p> <p>Results</p> <p>Nutrition has high influence on the antibiotic production of <it>X. bovienii</it> YL002. Glycerol and soytone were identified as the best carbon and nitrogen sources that significantly affected the antibiotic production using one-factor-at-a-time approach. Response surface methodology (RSM) was applied to optimize the medium constituents (glycerol, soytone and minerals) for the antibiotic production of <it>X. bovienii</it> YL002. Higher antibiotic activity (337.5 U/mL) was obtained after optimization. The optimal levels of medium components were (g/L): glycerol 6.90, soytone 25.17, MgSO<sub>4</sub>·7H<sub>2</sub>O 1.57, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> 2.55, KH<sub>2</sub>PO<sub>4</sub> 0.87, K<sub>2</sub>HPO<sub>4</sub> 1.11 and Na<sub>2</sub>SO<sub>4</sub> 1.81. An overall of 37.8% increase in the antibiotic activity of <it>X. bovienii</it> YL002 was obtained compared with that of the original medium.</p> <p>Conclusions</p> <p>To the best of our knowledge, there are no reports on antibiotic production of <it>X. boviebii</it> by medium optimization using RSM. The results strongly support the use of RSM for medium optimization. The optimized medium not only resulted in a 37.8% increase of antibiotic activity, but also reduced the numbers of experiments. The chosen method of medium optimization was efficient, simple and less time consuming. This work will be useful for the development of <it>X. bovienii</it> cultivation process for efficient antibiotic production on a large scale, and for the development of more advanced control strategies on plant diseases.</p
Planar carbon nanotube-graphene hybrid films for high-performance broadband photodetectors
Graphene has emerged as a promising material for photonic applications
fuelled by its superior electronic and optical properties. However, the
photoresponsivity is limited by the low absorption cross section and ultrafast
recombination rates of photoexcited carriers. Here we demonstrate a
photoconductive gain of 10 electrons per photon in a carbon
nanotube-graphene one dimensional-two dimensional hybrid due to efficient
photocarriers generation and transport within the nanostructure. A broadband
photodetector (covering 400 nm to 1550 nm) based on such hybrid films is
fabricated with a high photoresponsivity of more than 100 AW and a fast
response time of approximately 100 {\mu}s. The combination of ultra-broad
bandwidth, high responsivities and fast operating speeds affords new
opportunities for facile and scalable fabrication of all-carbon optoelectronic
devices.Comment: 21 pages, 3 figure
Disrupting ROS-protection mechanism allows hydrogen peroxide to accumulate and oxidize Sb(III) to Sb(V) in Pseudomonas stutzeri TS44
Primers used in this study. (DOC 35 kb
Observation of Small Polaron and Acoustic Phonon Coupling in Ultrathin La0.7Sr0.3MnO3/SrTiO3 Structures
Understanding the underlying physics of interactions among various quasi-particles is a fundamental issue for the application of spintronics and photonics. Here the observation of a coupling between the small polarons in the nanoscale ultrathin La0.7Sr0.3MnO3 (LSMO) films and the acoustic phonons in the SrTiO3 (STO) substrate using ultrafast pump–probe spectroscopy has been reported. According to the temperature- and wavelength-dependent measurements, the amplitudes of the acoustic phonons are suppressed by tuning the small polarons absorption. This shows a coupled relationship between the acoustic phonons and the small polarons. At the probe photon energy of 1.55 eV where the polaron absorption is dominant, the acoustic phonons become unobservable. Furthermore, by performing the pump fluence dependent measurements on the LSMO films with different thicknesses, smaller acoustic phonon amplitudes are found in the thinner film with stronger small polaron binding energy. Such a coupled nature can be utilized to manipulate the small polarons using the acoustic phonons or vice versa, which is of great importance in device applications of colossal magnetoresistance materials
A High-Performance Mid-infrared Optical Switch Enabled by Bulk Dirac Fermions in Cd3As2
Pulsed lasers operating in the 2-5 {\mu}m band are important for a wide range
of applications in sensing, spectroscopy, imaging and communications. Despite
recent advances with mid-infrared gain media, the lack of a capable pulse
generation mechanism, i.e. a passive optical switch, remains a significant
technological challenge. Here we show that mid-infrared optical response of
Dirac states in crystalline Cd3As2, a three-dimensional topological Dirac
semimetal (TDS), constitutes an ideal ultrafast optical switching mechanism for
the 2-5 {\mu}m range. Significantly, fundamental aspects of the photocarrier
processes, such as relaxation time scales, are found to be flexibly controlled
through element doping, a feature crucial for the development of convenient
mid-infrared ultrafast sources. Although various exotic physical phenomena have
been uncovered in three-dimensional TDS systems, our findings show for the
first time that this emerging class of quantum materials can be harnessed to
fill a long known gap in the field of photonics.Comment: 17 pages, 3 figure
Double-wall carbon nanotubes for wide-band, ultrafast pulse generation.
This is the final published version. It first appeared at http://pubs.acs.org/doi/abs/10.1021/nn500767b.We demonstrate wide-band ultrafast optical pulse generation at 1, 1.5, and 2 μm using a single-polymer composite saturable absorber based on double-wall carbon nanotubes (DWNTs). The freestanding optical quality polymer composite is prepared from nanotubes dispersed in water with poly(vinyl alcohol) as the host matrix. The composite is then integrated into ytterbium-, erbium-, and thulium-doped fiber laser cavities. Using this single DWNT-polymer composite, we achieve 4.85 ps, 532 fs, and 1.6 ps mode-locked pulses at 1066, 1559, and 1883 nm, respectively, highlighting the potential of DWNTs for wide-band ultrafast photonics.We acknowledge funding from EPSRC GR/
S97613/01, EP/E500935/1, the ERC Grant NANOPOTS, a Royal
Society Brian Mercer Award for Innovation. A.C.F. is a Royal
Society Wolfson Research Merit Award holder. V.N. wishes to
acknowledge support from the European Research Council (ERC
Starting Grant 2DNanoCaps) and Science Foundation Ireland,
P.T. from National Natural Science Foundation of China, Grants
No. 11225421, F.B. from the Newton International Fellowship,
Z.S. from Teknologiateollisuus TT-100, the European Union's
Seventh Framework Programme (No. 631610), and Aalto University, T.H. from NSFC (Grant No. 61150110487), and the Royal
Academy of Engineering (Graphlex)
Anisotropic ultrafast spin/valley dynamics in WTe2 films
WTe2 Weyl semimetal hosts the natural broken inversion symmetry and strong
spin orbit coupling, making it promising for exotic spin/valley dynamics within
a picosecond timescale. Here, we unveil an anisotropic ultrafast spin/valley
dynamics in centimeter-scale, single-crystalline Td-WTe2 films using a
femtosecond pump-probe technique at room temperature. We observe a transient
(~0.8 ps) intra-valley transition and a subsequent polarization duration (~5
ps) during the whole spin/valley relaxation process. Furthermore, the
relaxation exhibits the remarkable anisotropy of approximately six-fold and
two-fold symmetries due to the intrinsic anisotropy along the crystalline
orientation and the extrinsic matrix element effect, respectively. Our results
offer a prospect for the ultrafast manipulation of spin/valleytronics in
topological quantum materials for dissipationless high-speed spin/valleytronic
devices.Comment: 21 pages, 4 figure
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