Effect of Aqueous Extract from Morinda officinalis F. C. How

The present study aimed to assess the protective effects of aqueous extract from Morinda officinalis F. C. How on microwave-induced reproductive impairment in male rats. Microwave exposure injury was induced by exposure of 900 MHz microwaves at 218 μm/cm2 radiation densities, 24 hours/day for 10 days. Male Sprague-Dawley rats were randomized to: normal control, microwave exposure model, or water layer or ethyl acetate layer of aqueous extract 40 g/kg treatment groups. After 2 weeks of treatment, sexual performance, serum levels of gonadotrophin-releasing hormone (GnRH), luteinizing hormone (LH), follicle-stimulating hormone (FSH) or testosterone, morphological analysis of testis and epididymis, and GnRH protein expression in the hypothalamus were measured. Pretreatment with water layer of aqueous extract 40 g/kg significantly improved sexual performance, increased serum testosterone level, and decreased LH and GnRH level compared with microwave exposed model rats (all P<0.05). Water layer of aqueous extract treatment significantly increased seminiferous cell or sperm number in testis and epididymis. Protein expression of GnRH in the hypothalamus significantly decreased in the water layer of aqueous extract treated group (P<0.05). Ethyl acetate layer of aqueous extract did not show obvious effects on the measured parameters. These findings suggest that water layer of aqueous extract 40 g/kg ameliorates microwave-reduced reproductive impairment

Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles

Nanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well as autophagy, a lysosomal degradative pathway, is still not well known. We have previously reported in the 1321N1 cell line that amine-modified polystyrene (NH2-PS) NPs induce apoptosis through damage initiated in the lysosomes leading ultimately to release of lysosomal content in the cytosol, followed by apoptosis. Here, by using a combination of biochemical and cell biological approaches, we have characterized in a mouse embryonic fibroblast cell line that the lysosomal alterations induced by NH2-PS NPs is progressive, initiating from mild lysosomal membrane permeabilization (LMP), to expansion of lysosomal volume and intensive LMP before the summit of cell death. Though the cells initially seem to induce autophagy as a surviving mechanism, the damage of NH2-PS NPs to lysosomes probably results in lysosomal dysfunctions, leading to blockage of autophagic flux at the level of lysosomes and the eventual cell death

3-(4-Bromo­phen­yl)-4-(4-hy­droxy­anilino)furan-2(5H)-one

In the title compound, C16H12BrNO3, the butyrolactone core adopts the furan-2(5H)-one structure and forms dihedral angles of 44.80 (17) and 65.73 (18)° with the bromo­benzene and phenol rings, respectively. In the crystal, N—H⋯O and O—H⋯O hydrogen bonds link the mol­ecules, generating R 4 3(26) loops The edge-fused rings extend to form a chain running along the b-axis direction and C—H⋯π contacts help to consolidate the packing

Automatic Detection, Validation and Repair of Race Conditions in Interrupt-Driven Embedded Software

Interrupt-driven programs are widely deployed in safety-critical embedded systems to perform hardware and resource dependent data operation tasks. The frequent use of interrupts in these systems can cause race conditions to occur due to interactions between application tasks and interrupt handlers (or two interrupt handlers). Numerous program analysis and testing techniques have been proposed to detect races in multithreaded programs. Little work, however, has addressed race condition problems related to hardware interrupts. In this paper, we present SDRacer, an automated framework that can detect, validate and repair race conditions in interrupt-driven embedded software. It uses a combination of static analysis and symbolic execution to generate input data for exercising the potential races. It then employs virtual platforms to dynamically validate these races by forcing the interrupts to occur at the potential racing points. Finally, it provides repair candidates to eliminate the detected races. We evaluate SDRacer on nine real-world embedded programs written in C language. The results show that SDRacer can precisely detect and successfully fix race conditions.Comment: This is a draft version of the published paper. Ke Wang provides suggestions for improving the paper and README of the GitHub rep

Research on the Brand Niche Overlap of the B2C Websites: A Case of four Online Cosmetics Stores

This paper uses theories in ecology for resource competition to study the brand competition in business. It provides a quantitative method to measure brand breadth and brand overlap and examines their relationships with brand competition. Four Chinese online Cosmetics stores are used as examples to illustrate the utility of the proposed method in evaluating their degree of competition. This study can help companies identify main competitors in the industry and the main aspects that they are competing in. Key words: Brand niche; Brand niche breadth; Brand niche overlap; Online cosmetics stores bran

Manipulating autophagic processes in autoimmune diseases: a special focus on modulating chaperone-mediated autophagy, an emerging therapeutic target.

Autophagy, a constitutive intracellular degradation pathway, displays essential role in the homeostasis of immune cells, antigen processing and presentation, and many other immune processes. Perturbation of autophagy has been shown to be related to several autoimmune syndromes, including systemic lupus erythematosus. Therefore, modulating autophagy processes appears most promising for therapy of such autoimmune diseases. Autophagy can be said non-selective or selective; it is classified into three main forms, namely macroautophagy, microautophagy, and chaperone-mediated autophagy (CMA), the former process being by far the most intensively investigated. The role of CMA remains largely underappreciated in autoimmune diseases, even though CMA has been claimed to play pivotal functions into major histocompatibility complex class II-mediated antigen processing and presentation. Therefore, hereby, we give a special focus on CMA as a therapeutic target in autoimmune diseases, based in particular on our most recent experimental results where a phosphopeptide modulates lupus disease by interacting with CMA regulators. We propose that specifically targeting lysosomes and lysosomal pathways, which are central in autophagy processes and seem to be altered in certain autoimmune diseases such as lupus, could be an innovative approach of efficient and personalized treatment.journal articlereview20152015 05 19importe