Concepción, guion literario y desarrollo narrativo del cortometraje de ficción “Vecinos extraños”.

Este trabajo presenta la concepción, el guion literario y el desarrollo narrativo del cortometraje de ficción audiovisual y de creación propia “Vecinos extraños”. A lo largo de la memoria de TFM el lector puede consultar el método seguido para la elaboración del cortometraje (incluyendo la formación del equipo de rodaje y los materiales de filmación), así como un desglose minucioso de las fases de preproducción, rodaje y postproducción. Posteriormente, detallamos la experiencia personal que ha supuesto para cada uno de los autores (Feng Yuxuan y Qin Xuyang) la elaboración de este TFM como punto culminante de un año inmersos en el Máster de Cine, Comunicación e Industria Audiovisual de la Universidad de Valladolid. Por último, el lector puede consultar el guion literario íntegro de “Vecinos extraños” en formato profesional de guion y, por supuesto, visionar el cortometraje a través del enlace facilitado para ello.Departamento de Historia Moderna, Contemporánea y de América, Periodismo y Comunicación Audiovisual y PublicidadMáster en Cine, comunicación e Industria Audiovisua

Authentication method of sea duck eggs based on targeted metabolomics

Objective A targeted metabolomics based on gas chromatography-mass spectrometry (GC-MS) was established for the authentication of sea duck eggs. Methods In this study, gas chromatography-mass spectrometry was used to characterize the fat acid profiles of sea duck eggs and cage duck eggs. Chemometrics was used to analyze the difference between sea duck eggs and cage duck eggs to screen the markers that could be used to identify the authenticity of sea duck eggs. Results Omega-3 fatty acids (Ω-3) in sea duck eggs account for a higher proportion of total fatty acids than cage duck eggs, and there were significant differences (t=23.58, P<0.05). Sea duck eggs and cage duck eggs were distinguished by principal component analysis. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were screened by orthogonal partial least squares discriminant analysis that contributed the most to the difference of sea duck eggs and cage duck eggs. EPA was detected only in sea duck eggs, and the content of DHA in sea duck eggs was higher than that of cage duck eggs. Conclusion This study showed that EPA and DHA can be used as a reliable marker to authenticate sea duck eggs

Population pharmacokinetics of nalbuphine in patients undergoing general anesthesia surgery

Purpose: The aim of this study was to build a population pharmacokinetics (PopPK) model of nalbuphine and to estimate the suitability of bodyweight or fixed dosage regimen.Method: Adult patients who were undergoing general anesthetic surgery using nalbuphine for induction of anesthesia were included. Plasma concentrations and covariates information were analyzed by non-linear mixed-effects modeling approach. Goodness-of-fit (GOF), non-parametric bootstrap, visual predictive check (VPC) and external evaluation were applied for the final PopPK model evaluation. Monte Carlo simulation was conducted to assess impact of covariates and dosage regimens on the plasma concentration to nalbuphine.Results: 47 patients aged 21–78 years with a body weight of 48–86 kg were included in the study. Among them, liver resection accounted for 14.8%, cholecystectomy for 12.8%, pancreatic resection for 36.2% and other surgeries for 36.2%. 353 samples from 27 patients were enrolled in model building group; 100 samples from 20 patients were enrolled in external validation group. The results of model evaluation showed that the pharmacokinetics of nalbuphine was adequately described by a two-compartment model. The hourly net fluid volume infused (HNF) was identified as a significant covariate about the intercompartmental clearance (Q) of nalbuphine with objective function value (OFV) decreasing by 9.643 (p &lt; 0.005, df = 1). Simulation results demonstrated no need to adjust dosage based on HNF, and the biases of two dosage methods were less than 6%. The fixed dosage regimen had lower PK variability than the bodyweight regimen.Conclusion: A two-compartment PopPK model adequately described the concentration profile of nalbuphine intravenous injection for anesthesia induction. While HNF can affect the Q of nalbuphine, the magnitude of the effect was limited. Dosage adjustment based on HNF was not recommended. Furthermore, fixed dosage regimen might be better than body weight dosage regimen

Peliminary exploration on the differential diagnosis between meningioma and schwannoma using contrast-enhanced T1WI flow-sensitive black-blood sequence

IntroductionContrast-enhanced T1WI flow-sensitive black-blood (CE-T1WI FSBB) is a newly developed sequence which had not been widely used for differential diagnosis of brain tumors.MethodsTo quantify the pre-operative imaging features of intratumoral microbleeds and intratumoral vessels using CE-T1WI FSBB scan and study the differences in biological behavior of meningiomas and schwannomas underlying the imaging features. Seventy-three cases of meningiomas and 24 cases of schwannomas confirmed by postoperative pathology were included. Two neuroradiologists independently counted intratumoral vessels and intratumoral microbleeds based on CE-T1WI FSBB images. The vessel density index (VDI) and microbleed density index (MDI) were the number of intratumoral vessels and the number of intratumoral microbleeds divided by the tumor volume, respectively. The consistency test of intratumoral vessel count and intratumoral microbleed count based on CE-T1WI FSBB were summarized using 2-way random intraclass correlation coefficients (ICC). Mann–Whitney U-test and chi-square test were used to determine significant differences between meningiomas and schwannomas, and fibrous meningiomas and epithelial meningiomas. P&lt;0.05 was considered statistically significant.ResultsThe ICC of intratumoral vessels count and intratumoral microbleeds count were 0.89 and 0.99, respectively. There were significant differences in the number of intratumoral microbleeds (P&lt;0.01) and MDI values (P&lt;0.01) between meningiomas and schwannomas. There were no differences in the number of intratumoral vessels (P=0.64), VDI (P=0.17), or tumor volume (P=0.33). There were also differences in the number of intratumoral microbleeds (P&lt;0.01), the MDI value (P&lt;0.01), and the sex of patients (P&lt;0.05) between fibrous meningiomas and epithelial meningiomas.DiscussionCE-T1WI FSBB can be a new technique for differentiating schwannomas from meningiomas, and even different types of meningiomas. Schwannomas have a higher incidence of intratumoral hemorrhage, more intratumoral microbleeds, and higher MDI values than meningiomas, which provides a new basis for preoperative differential diagnosis and treatment decisions