Development and Validation of a Questionnaire for Analyzing Real-Life Falls in Long-Term Care Captured On Video

Background Falls are the number one cause of injuries in older adults, and are particularly common in long-term care (LTC). Lack of objective evidence on the mechanisms of falls in this setting is a major barrier to prevention. Video capture of real-life falls can help to address this barrier, if valid tools are available for data analysis. To address this need, we developed a 24-item fall video analysis questionnaire (FVAQ) to probe key biomechanical, behavioural, situational, and environmental aspects of the initiation, descent, and impact stages of falls. We then tested the reliability of this tool using video footage of falls collected in LTC. Methods Over three years, we video-captured 221 falls experienced by 130 individuals in common areas (e.g., dining rooms, hallways, and lounges) of two LTC facilities. The FVAQ was developed through literature review and an iterative process to ensure our responses captured the most common behaviours observed in preliminary review of fall videos. Inter-rater reliability was assessed by comparing responses from two teams, each having three members, who reviewed 15 randomly-selected videos. Intra-rater reliability was measured by comparing responses from one team at baseline and 12 months later. Results In 17 of the 24 questions, the percentage of inter- and intra-rater agreement was over 80% and the Cohen\u27s Kappa was greater than 0.60, reflecting good reliability. These included questions on the cause of imbalance, activity at the time of the fall, fall direction, stepping responses, and impact to specific body sites. Poorer agreement was observed for footwear, contribution of clutter, reach-to-grasp responses, and perceived site of injury risk. Conclusions Our results provide strong evidence of the reliability of the FVAQ for classifying biomechanical, behavioural, situational, and environmental aspects of falls captured on video in common areas in LTC. Application of this tool should reveal new and important strategies for the prevention and treatment of falls and fall-related injuries in this setting

The Monge problem in Wiener Space

We address the Monge problem in the abstract Wiener space and we give an existence result provided both marginal measures are absolutely continuous with respect to the infinite dimensional Gaussian measure {\gamma}

Coronal fuzziness modelled with pulse-heated multistranded loop systems

Coronal active regions are observed to get fuzzier and fuzzier (i.e. more and more confused and uniform) in harder and harder energy bands or lines. We explain this evidence as due to the fine multi-temperature structure of coronal loops. To this end, we model bundles of loops made of thin strands, each heated by short and intense heat pulses. For simplicity, we assume that the heat pulses are all equal and triggered only once in each strand at a random time. The pulse intensity and cadence are selected so as to have steady active region loops ($\sim 3$ MK), on the average. We compute the evolution of the confined heated plasma with a hydrodynamic loop model. We then compute the emission along each strand in several spectral lines, from cool ($\leq 1$ MK), to warm ($2-3$ MK) lines, detectable with Hinode/EIS, to hot X-ray lines. The strands are then put side-by-side to construct an active region loop bundle. We find that in the warm lines ($2-3$ MK) the loop emission fills all the available image surface. Therefore the emission appears quite uniform and it is difficult to resolve the single loops, while in the cool lines the loops are considerably more contrasted and the region is less fuzzy. The main reasons for this effect are that, during their evolution, i.e. pulse heating and slow cooling, each strand spends a relatively long time at temperatures around $2-3$ MK, and that it has a high emission measure during that phase, so the whole region appears more uniform or smudged. We make the prediction that the fuzziness should be reduced in the hot UV and X-ray lines.Comment: 27 pages, 14 figure

The Italian genome reflects the history of Europe and the Mediterranean basin

Recent scientific literature has highlighted the relevance of population genetic studies both for disease association mapping in admixed populations and for understanding the history of human migrations. Deeper insight into the history of the Italian population is critical for understanding the peopling of Europe. Because of its crucial position at the centre of the Mediterranean basin, the Italian peninsula has experienced a complex history of colonization and migration whose genetic signatures are still present in contemporary Italians. In this study, we investigated genomic variation in the Italian population using 2.5 million single-nucleotide polymorphisms in a sample of more than 300 unrelated Italian subjects with well-defined geographical origins. We combined several analytical approaches to interpret genome-wide data on 1272 individuals from European, Middle Eastern, and North African populations. We detected three major ancestral components contributing different proportions across the Italian peninsula, and signatures of continuous gene flow within Italy, which have produced remarkable genetic variability among contemporary Italians. In addition, we have extracted novel details about the Italian population's ancestry, identifying the genetic signatures of major historical events in Europe and the Mediterranean basin from the Neolithic (e.g., peopling of Sardinia) to recent times (e.g., ‘barbarian invasion' of Northern and Central Italy). These results are valuable for further genetic, epidemiological and forensic studies in Italy and in Europe

Temperature distribution of a non-flaring active region from simultaneous Hinode XRT and EIS observations

We analyze coordinated Hinode XRT and EIS observations of a non-flaring active region to investigate the thermal properties of coronal plasma taking advantage of the complementary diagnostics provided by the two instruments. In particular we want to explore the presence of hot plasma in non-flaring regions. Independent temperature analyses from the XRT multi-filter dataset, and the EIS spectra, including the instrument entire wavelength range, provide a cross-check of the different temperature diagnostics techniques applicable to broad-band and spectral data respectively, and insights into cross-calibration of the two instruments. The emission measure distribution, EM(T), we derive from the two datasets have similar width and peak temperature, but show a systematic shift of the absolute values, the EIS EM(T) being smaller than XRT EM(T) by approximately a factor 2. We explore possible causes of this discrepancy, and we discuss the influence of the assumptions for the plasma element abundances. Specifically, we find that the disagreement between the results from the two instruments is significantly mitigated by assuming chemical composition closer to the solar photospheric composition rather than the often adopted "coronal" composition (Feldman 1992). We find that the data do not provide conclusive evidence on the high temperature (log T[K] >~ 6.5) tail of the plasma temperature distribution, however, suggesting its presence to a level in agreement with recent findings for other non-flaring regions.Comment: 14 pages, 15 figures. Accepted for publication in the Astrophysical Journa

The late Pleistocene Po River lowstand wedge in the Adriatic Sea : Controls on architecture variability and sediment partitioning

The authors dedicate this study to their colleague Giovanni Bortoluzzi, who passed away in 2015. A special tanks is due to Marco Ligi and Nevio Zitellini for geophysical data acquisition and processing; Marco Pastore and Filippo D'Oriano for their support during the cruise LSD2014 and processing of geophysical data. Elisabetta Campiani provided additional support for processing the multibeam bathymetry. A particular thank goes to Cpt. Emanuele Gentile and the crew of the R/V Urania during cruise LSD 2014. We thank Ronald Steel and an anonymous Reviewer for their constructive comments. This project was funded by ExxonMobil Upstream Research Company and by the Flagship Project RITMARE–The Italian Research for the Sea. We acknowledge the European Union Project PROMESS-1 (contract EVR1-2001-41) for borehole PRAD 1-2. This is ISMAR-CNR contribution number 1959.Peer reviewedPostprin

Análise da preservação do miocárdio após administração de N-2 mercaptopropionilglicina em modelo experimental de isquemia e reperfusão em cães

Nowadays, cardiovascular diseases are the principal cause of morbimortality, specially the coronary artery disease. New physiopathological acknowledgement indicates new mechanisms for myocardial injury, still not quite studied.The aim of this study is to analyze the effect of N2-Mercaptopropionilglicine (N2-MPG) as a myocardial protection agent during ischemya/reperfusion based on the preservationof the necrosis area. Sixteen dogs where divided into two groups of eight dogs each one: Group I (N2-MPG) and Group II (control). After the experimental acute myocardialinfarction reproduction, the hearts where colored (TTC/Blue Evans dye) and weighed for the myocardial preservation analysis. The amount of necrosis area were: Group I(73,14%) and Group II (49,98%) through the Mann-Whitney method. The necrosis area reduction showed an average reduction of 23,16%, concluding that N2-MPG has aprotection effect during myocardial ischemya/reperfusion.As doenças cardiovasculares representam a principal causa de morbi-mortalidade da atualidade, sendo a doença arterial coronariana seu maior expoente. Novos conhecimentosfisiopatológicos indicam outros mecanismos para injúria miocárdica, ainda pouco estudados. O objetivo desse trabalho é analisar o efeito da N2-Mercaptopropionilglicina (N2-MPG) como agente protetor do miocárdio durante a isquemia/reperfusão, com base na preservação da área denecrose. Foram utilizados 16 cães alocados em dois grupos de oito cães cada: Grupo I (N2-MPG) e Grupo II (controle). Após a reprodução experimental de infarto agudo do miocárdio, os corações foram corados (TTC/Azul de Evans) e pesados para análise da preservação do miocárdio. Os resultados, em relação à preservação da área de necrose, foram: Grupo I (73,14%) e Grupo II (49,98%) através do método de Mann-Whitney. A redução da área de necrose obtida neste estudo apresentou diferença estatística significativa entre os grupos mostrando uma reduçãomédia de 23,16%, concluindo-se que a N2-MPG possui efeito protetor durante a isquemia/reperfusão do miocárdio

Short telomeres in ESCs lead to unstable differentiation

SummaryFunctional telomeres are critical for stem cell proliferation; however, whether they are equally important for the stability of stem cell differentiation is not known. We found that mouse embryonic stem cells (ESCs) with critically short telomeres (Tert−/− ESCs) initiated normal differentiation after leukemia inhibitory factor (LIF) withdrawal but, unlike control ESCs, failed to maintain stable differentiation when LIF was reintroduced to the growth medium. Tert−/− ESCs expressed higher levels of Nanog and, overall, had decreased genomic CpG methylation levels, which included the promoters of Oct4 and Nanog. This unstable differentiation phenotype could be rescued by telomere elongation via reintroduction of Tert, via suppression of Nanog by small hairpin RNA (shRNA) knockdown, or via enforced expression of the de novo DNA methyltransferase 3b. These results demonstrate an unexpected role of functional telomeres in the genome-wide epigenetic regulation of cell differentiation and suggest a potentially important role of telomere instability in cell fate during development or disease

An Overview of Marine Biodiversity in United States Waters

Marine biodiversity of the United States (U.S.) is extensively documented, but data assembled by the United States National Committee for the Census of Marine Life demonstrate that even the most complete taxonomic inventories are based on records scattered in space and time. The best-known taxa are those of commercial importance. Body size is directly correlated with knowledge of a species, and knowledge also diminishes with distance from shore and depth. Measures of biodiversity other than species diversity, such as ecosystem and genetic diversity, are poorly documented. Threats to marine biodiversity in the U.S. are the same as those for most of the world: overexploitation of living resources; reduced water quality; coastal development; shipping; invasive species; rising temperature and concentrations of carbon dioxide in the surface ocean, and other changes that may be consequences of global change, including shifting currents; increased number and size of hypoxic or anoxic areas; and increased number and duration of harmful algal blooms. More information must be obtained through field and laboratory research and monitoring that involve innovative sampling techniques (such as genetics and acoustics), but data that already exist must be made accessible. And all data must have a temporal component so trends can be identified. As data are compiled, techniques must be developed to make certain that scales are compatible, to combine and reconcile data collected for various purposes with disparate gear, and to automate taxonomic changes. Information on biotic and abiotic elements of the environment must be interactively linked. Impediments to assembling existing data and collecting new data on marine biodiversity include logistical problems as well as shortages in finances and taxonomic expertise

Association between Sedentary Behaviour and Physical, Cognitive, and Psychosocial Status among Older Adults in Assisted Living

Objective. Identification of the factors that influence sedentary behaviour in older adults is important for the design of appropriate intervention strategies. In this study, we determined the prevalence of sedentary behaviour and its association with physical, cognitive, and psychosocial status among older adults residing in Assisted Living (AL). Methods. Participants (, mean age = 86.7) from AL sites in British Columbia wore waist-mounted activity monitors for 7 consecutive days, after being assessed with the Timed Up and Go (TUG), Montreal Cognitive Assessment (MoCA), Short Geriatric Depression Scale (GDS), and Modified Fall Efficacy Scale (MFES). Results. On average, participants spent 87% of their waking hours in sedentary behaviour, which accumulated in 52 bouts per day with each bout lasting an average of 13 minutes. Increased sedentary behaviour associated significantly with scores on the TUG (, ) and MFES (, ), but not with the MoCA or GDS. Sedentary behaviour also associated with male gender, use of mobility aid, and multiple regression with increased age. Conclusion. We found that sedentary behaviour among older adults in AL associated with TUG scores and falls-related self-efficacy, which are modifiable targets for interventions to decrease sedentary behaviour in this population