Characteristic amino acids in tea leaves as quality indicator for evaluation of Wuyi Rock Tea in different cultured regions

Free amino acid compositions in Wuyi Rock Tea leaves from Yu (authentic rock region), Guiyan (semi-authentic rock region) and Qishan (ordinary region) tea plantations were analyzed. Results showed that contents of 18 free amino acids were 1.6-2.0 times higher in Yu and Guiyan than that in Qishan. The theanine contents reached to 17-20 mg g-1 in Yu and Guiyan, while it was less than 10 mg g-1 in Qishan. Hierarchical cluster analysis and principal component analysis were effective in distinguishing Rock Tea from different regions. The ratios of theanine, sweet and umami amino acids were 8%, 5% and 6% higher, respectively in Yu than that in Qishan. Sensory evaluation score were positively correlated with the ratios of theanine, sweet and umami amino acids (P < 0.05 or P < 0.01). Our results highlight that the favourite characteristic amino acids are dominant contributors to sweet aftertaste of Rock Tea

Optimizing Broadband Near-Infrared Emission in Bi/Sn-Doped Aluminosilicate Glass by Modulating Glass Composition

The Bi/Sn-doped aluminosilicate glass samples were prepared using a melting–quenching method and their near-infrared (NIR) emission properties were studied. An ultra-broadband NIR emission ranging from 950 nm to 1600 nm was observed in all samples under 480 nm excitation, which covered the whole fiber low-loss window. The NIR emission spectrum showed that the maximum emission peak was about 1206 nm and the full width at half maximum (FWHM) was about 220 nm. Furthermore, the NIR emission intensity strongly depends on the composition of the glass, which can be optimized by modulating the glass composition. The Bi0 and Bi+ ions were the NIR luminescence source of the glass samples in this paper. The Bi/Sn-doped aluminosilicate glass has the potential to become a new type of core fiber material and to be applied to optical fiber amplifiers (OFAs), based on its excellent performance in ultra-broadband NIR emission

CMB power spectrum in the emergent universe with k-essence

The emergent universe provides a possible method to avoid the big bang singularity by considering that the universe stems from an stable Einstein static universe rather than the singularity. Since the Einstein static universe exists before inflation, it may leave some relics in the CMB power spectrum. In this paper, we analyze the stability condition for the Einstein static universe in general relativity with k-essence against both the scalar and tensor perturbations. And we find the emergent universe can be successfully realized by constructing a scalar potential and an equation of state parameter. Solving the curved Mukhanov-Sasaki equation, we obtain the analytical approximation for the primordial power spectrum, and then depict the TT-spectrum of the emergent universe. The results show that both the primordial power spectrum and CMB TT-spectrum are suppressed on large scales

Aggregation of ODC(I) and POL Defects in Bismuth Doped Silica Fiber

First-principles calculations were used to simulate the aggregation of the peroxy chain defect POL and the oxygen vacancy defect ODC(I). Defect aggregation&rsquo;s electronic structure and optical properties were investigated. The two defects were most likely to accumulate on a 6-membered ring in ortho-position. When the two defects are aggregated, it is discovered that 0.75 ev absorption peaks appear in the near-infrared band, which may be brought on by the addition of oxygen vacancy defect ODC(I). We can draw the conclusion that the absorption peak of the aggregation defect of ODC(I) defect and POL is more prominent in the near infrared region and visible light area than ODC(I) defect and POL defect

Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines.

BACKGROUND: Wheat-rye addition lines are an old topic. However, the alterations and abnormal mitotic behaviours of wheat chromosomes caused by wheat-rye monosomic addition lines are seldom reported. METHODOLOGY/PRINCIPAL FINDINGS: Octoploid triticale was derived from common wheat T. aestivum L. 'Mianyang11'×rye S. cereale L. 'Kustro' and some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' followed by self-fertilization. Genomic in situ hybridization (GISH) using rye genomic DNA and fluorescence in situ hybridization (FISH) using repetitive sequences pAs1 and pSc119.2 as probes were used to analyze the mitotic chromosomes of these progeny. Strong pSc119.2 FISH signals could be observed at the telomeric regions of 3DS arms in 'Mianyang11'. However, the pSc119.2 FISH signals were disappeared from the selfed progeny of 4R monosomic addition line and the changed 3D chromosomes could be transmitted to next generation stably. In one of the selfed progeny of 7R monosomic addition line, one 2D chromosome was broken and three 4A chromosomes were observed. In the selfed progeny of 6R monosomic addition line, structural variation and abnormal mitotic behaviour of 3D chromosome were detected. Additionally, 1A and 4B chromosomes were eliminated from some of the progeny of 6R monosomic addition line. CONCLUSIONS/SIGNIFICANCE: These results indicated that single rye chromosome added to wheat might cause alterations and abnormal mitotic behaviours of wheat chromosomes and it is possible that the stress caused by single alien chromosome might be one of the factors that induced karyotype alteration of wheat

Conserved DNA sequence analysis reveals the phylogeography and evolutionary events of Akebia trifoliata in the region across the eastern edge of the Tibetan Plateau and subtropical China

Abstract Background The eastern edge of the Qinghai‒Tibet Plateau (QTP) and subtropical China have various regions where plant species originate and thrive, but these regions have been the focus of very few integrative studies. Here, we elucidated the phylogeographic structure of a continuous and widespread Akebia trifoliata population across these two regions. Results Sixty-one populations consisting of 391 genotypes were examined to assess population diversity and structure via network distribution analysis, maximum likelihood phylogenetic tree reconstruction, divergence time estimation, demographic history inference, and ancestral area reconstruction of both conserved internal transcribed spacer (ITS) and chloroplast (rps16) DNA sequences. The results showed that the ITS region was more variable than the rps16 region and could be suitable for studying intraspecific phylogeography. The A. trifoliata population displayed high genetic diversity, genetic differentiation and obvious phylogeographical structure, possibly originating on the eastern QTP, expanding during the last glacial-interglacial cycle, diverging in the early Pleistocene and middle Pleistocene, and extensively migrating thereafter. The migration route from west to east along rivers could be largely responsible for the long-distance dispersal of this species, while three main refuges (Qinba Mountains, Nanling Mountains and Yunnan-Guizhou Plateau) with multiple ice shelters facilitated its wide distribution. Conclusions Our results suggested that the from west to east long migration accompanying with the minor short reciprocal migration in the south-north direction, and the three main refuges (the Qinba Mountains, Nanling Mountains and Yunnan-Guizhou Plateau) contributed to the extant geographical distribution of A. trifoliata. In addition, this finding also strongly reduced the discrepancy between glacial contraction and postglacial expansion and the in situ survival hypothesis by simultaneously considering the existence of many similar climate-related ecological niches and migration influences

Expression Profiles of Microsatellites in Fruit Tissues of Akebia trifoliata and Development of Efficient EST-SSR Markers

Akebia trifoliata, a member of the family Lardizabalaceae, has high exploitation potential for multiple economic purposes, so genetic improvements to meet requirements for commercial demand are needed. However, this progress is largely impeded by a lack of effective selection markers. In this study, we obtained 271.49 Gb of clean transcriptomic data from 12 samples (three tissues at four developmental stages) of A. trifoliata fruit. We identified 175,604, 194,370, and 207,906 SSRs from the de novo assembled 416,363, 463,756, and 491,680 unigene sequences obtained from the flesh, seed, and rind tissues, respectively. The profile and proportion of SSR motifs expressed in each fruit tissue and developmental stage were remarkably similar, but many trinucleotide repeats had differential expression levels among different tissues or at different developmental stages. In addition, we successfully designed 16,869 functional EST-SSR primers according to the annotated unigenes. Finally, 94 and 72 primer pairs out of 100 randomly selected primer pairs produced clear bands and polymorphic bands, respectively. These results were also used to elucidate the expression profiles of different tissues at various stages. Additionally, we provided a set of effective, polymorphic, and reliable EST-SSR markers sufficient for accelerating the discovery of metabolic and pathway-specific functional genes for genetic improvement and increased commercial productivity

Additional file 1 of Conserved DNA sequence analysis reveals the phylogeography and evolutionary events of Akebia trifoliata in the region across the eastern edge of the Tibetan Plateau and subtropical China

Supplementary Table S1: Locations of populations of Akebia trifoliata sampled, sample sizes(n), the cpDNA haplotype and ITS haplotype frequency, haplotype distribution type, haplotype (gene) diversity (Hd) and nucleotide diversity (π × 10 − 3) of each populatio

FISH and GISH using pAs1 (red), pSc119.2 (green) and rye genomic DNA (red) as probes on 7R monosomic addition line and its selfed progeny.

<p><b>A</b> FISH and GISH analyses on the chromosomes of MK7RF₄. <b>B</b> FISH and GISH analyses on the chromosomes of one of the selfed progeny of MK7RF₄ and GISH signals can not be observed. The three 4A chromosomes are marked. Arrow indicates the broken 2D chromosome. Chromosomes were counterstained with DAPI (blue).</p

FISH and GISH using pAs1 (red), pSc119.2 (green) and rye genomic DNA (red) as probes on 6R monosomic addition line and its selfed progeny.

<p><b>A</b> FISH and GISH analyses on the chromosomes of MK6RF₄. <b>B</b> One 4B chromosome is eliminated in wheat line 6RMYF₅-1. <b>C</b> Both of the 4B chromosomes are eliminated in wheat line 6RMYF₅-5. <b>D</b> Three 3D chromosomes are observed and one 1A chromosome is eliminated in wheat line 6RMYF₅-8. <b>E</b> One 1A chromosome is eliminated in wheat line 6RMYF₅-8. <b>F</b> Seven 3DS isochromosomes and a broken D-genome chromosome are observed in wheat line 6RMYF₅-28. Arrows indicate the 3DS isochromosomes. Triangle indicates the broken D-genome chromosome. No GISH signals were observed in B, C, D, E and F. Chromosomes were counterstained with DAPI (blue).</p