Linkage and Association Mapping of Seed Size and Shape in Lentil

The seed size and shape of lentil are important traits because they determine the market class, cooking time, and can influence quality and yield of milled lentils. Understanding the genetic control of seed size and shape can help breeders develop varieties with improved seed size and shape characteristics such as seed diameter, seed thickness and seed plumpness. The objectives were to determine the heritability of seed size and shape and identify the genomic regions controlling these traits. This involved i) developing a linkage map for the LR-18 population (CDC Robin x 964a-46) using a recently developed single nucleotide polymorphism (SNP) assay; ii) analyzing the LR-18 population for seed size and shape QTLs; iii) analyzing an association mapping panel for seed size and shape QTLs. Phenotyping trials were grown at two different locations in Saskatchewan, Canada. The mapping population was grown in two different years while the association panel was only grown in one. Seed diameter and thickness were measured using sieves and this data were used to calculate seed plumpness. Days to flowering was also recorded to determine if it had any effect on seed size or shape. A linkage map consisting of 537 SNPs, 10 SSRs and 4 morphological markers on seven linkage groups was constructed and used for the QTL analysis. The heritability estimates were high for seed diameter and seed plumpness (0.92 and 0.94, respectively) while for seed thickness and days to flowering they were more moderate (0.60 and 0.45, respectively). QTL analysis revealed QTLs on five of the seven linkage groups. The association mapping study revealed similar heritability estimates of 0.97, 0.62, 0.94, and 0.62 for seed diameter, seed thickness, seed plumpness and flowering time, respectively. There were 31 different significant marker trait associations, however only 5 of those were significant for both locations. Four of those five markers did not map in the LR-18 linkage map so their genomic locations are still to be determined. Results showed that there are key regions in the genome that control seed size and shape and flowering time in lentil. These markers could be used for marker-assisted selection or for further candidate gene analysis

Twisted light Michelson interferometer for high precision refractive index measurements

Using orbital angular momentum beams in a Michelson interferometer opens the possibility for non-invasive measurements of refractive index changes down to 10(-6) refractive index units. We demonstrate the application of a twisted light interferometer to directly measure the concentration of NaCl and glucose solutions label-free and in situ and to monitor temperature differences in the mK-mu K range. From these measurements we can extract a correlation of the refractive index to concentration and to temperature from a liquid sample which is in good agreement with literature. Applying this type of twisted light interferometry yields a novel, robust, and easily implementable method for in situ monitoring of concentration and temperature changes in microfluidic samples. (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreemen

The Relative Importance of Academic Activities: Autonomous Values from the Canadian Professoriate

In this paper, we report findings on the contemporary idea of a university as defined by the relative importance of teaching, research, service, and collegiality in teaching-intensive universities. We also investigate Boyer’s model of scholarship relative to the research–teaching–service nexus. Our findings show that while research is embraced as a key academic responsibility of professors, there is general agreement on the primacy of teaching. Equally, awareness of Boyer’s model exposes faculty to diverse opportunities for scholarship and has the potential to open up faculty members to a new idea of the university.  Dans cet article, nous présentons les résultats d’une recherche portant sur l’idée contemporaine de l’université, telle que définie par l’importance relative de l’enseignement, de la recherche, du service et de la collégialité dans des établissements d’enseignement intensif de niveau collégial ou universitaire. Notre recherche porte également sur le modèle boursier de Boyer, relatif au lien entre recherche, enseignement et service. Nos résultats démontrent que tandis qu’on voit la recherche comme une responsabilité académique des professeurs, il existe une entente générale quant à l’importance de l’enseignement. De même, le fait de connaître le modèle de Boyer, ouvre aux facultés d’enseignement la porte aux diverses opportunités d’obtention de bourses, et amène aussi à sensibiliser le personnel à une nouvelle pensée universitaire

Two-Color Laser Printing of Individual Gold Nanorods

We report on the deposition of individual gold nanorods from an optical trap using two different laser wavelengths. Laser light, not being resonant to the plasmon resonances of the nanorods, is used for stable trapping and in situ alignment of individual nanorods. Laser light, being resonant to the transversal mode of the nanorods, is used for depositing nanorods at desired locations. The power and polarization dependence of the process is investigated and discussed in terms of force balances between gradient and scattering forces, plasmonic heating, and rotational diffusion of the nanorods. This two-color approach enables faster printing than its one-color equivalent and provides control over the angular orientation (±16°) and location of the deposited nanorods at the single-nanorod level