Ecole d'apprentissage [rue de Babylone, confection de patrons de couture] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 98899Appartient à l’ensemble documentaire : Pho20RolImage de press

Individual percent reduction (Δ) of O₂ consumption in the 22 participants during total recovery time (6.5min) along a 20-min training session in the presence (WBV) or absence (no vibration) of whole body vibration.

<p>Δ was significantly higher in the presence of WBV. Data are adjusted by peak acceleration and order of treatment administration (WBV/no vibration vs. no vibration/WBV).</p

Representative picture of the experimental set up.

<p>The participant is performing a squat exercise in the condition currently adopted for healthy subjects in fitness centers.</p

WBV-associated changes in several outcome measurements in a group (n = 22) of exercising male participants.

<p>Data are mean±SD.</p

Luminescence of Eu³⁺ Activated CaF₂ and SrF₂ Nanoparticles: Effect of the Particle Size and Codoping with Alkaline Ions

Eu³⁺ doped CaF₂ and SrF₂ nanoparticles were synthesized through a facile hydrothermal technique, using citrate ions as capping agents and Na⁺ or K⁺ as charge compensator ions. A proper tuning of the reaction time can modulate the nanoparticle size, from few to several tens of nanometers. Analysis of EXAFS spectra indicate that the Eu³⁺ ions enter into the fluorite CaF₂ and SrF₂ structure as substitutional defects on the metal site. Laser site selective spectroscopy demonstrates that the Eu³⁺ ions are mainly accommodated in two sites with different symmetries. The relative site distribution for lanthanide ions depends on the nanoparticle size, and higher symmetry Eu³⁺ sites are prevalent for bigger nanoparticles. Eu³⁺ ions in high symmetry sites present lifetimes of the ⁵D₀ level around 27 ms, among the longest lifetimes found in the literature for Eu³⁺ activated materials. As a proof of concept of possible use of the Eu³⁺ activated alkaline-earth fluoride nanoparticles in nanomedicine, the red luminescence generated by two-photon absorption using pulsed laser excitation at 790 nm (in the first biological window) has been detected. The long Eu³⁺ lifetimes suggest that the present nanomaterials can be interesting as luminescent probes in time-resolved fluorescence techniques in biomedical imaging (e.g., FLIM) where fast autofluorescence is a drawback to avoid

Inhibition of <i>Pseudomonas aeruginosa</i> secreted virulence factors reduces lung inflammation in CF mice

<p><b>Background</b>: Cystic fibrosis (CF) lung infection is a complex condition where opportunistic pathogens and defective immune system cooperate in developing a constant cycle of infection and inflammation. The major pathogen, <i>Pseudomonas aeruginosa</i>, secretes a multitude of virulence factors involved in host immune response and lung tissue damage. In this study, we examined the possible anti-inflammatory effects of molecules inhibiting <i>P. aeruginosa</i> virulence factors.</p> <p><b>Methods</b>: Pyocyanin, pyoverdine and proteases were measured in bacterial culture supernatant from different <i>P. aeruginosa</i> strains. Inhibition of virulence factors by sub-inhibitory concentrations of clarithromycin and by protease inhibitors was evaluated. Lung inflammatory response was monitored by in vivo bioluminescence imaging in wild-type and CFTR-knockout mice expressing a luciferase gene under the control of a bovine IL-8 promoter.</p> <p><b>Results</b>: The amount of proteases, pyocyanin and pyoverdine secreted by P. aeruginosa strains was reduced after growth in the presence of a sub-inhibitory dose of clarithromycin. Intratracheal challenge with culture supernatant containing bacteria-released products induced a strong IL-8-mediated response in mouse lungs while lack of virulence factors corresponded to a reduction in bioluminescence emission. Particularly, sole inactivation of proteases by inhibitors Ilomastat and Marimastat also resulted in decreased lung inflammation.</p> <p><b>Conclusions</b>: Our data support the assumption that virulence factors are involved in <i>P. aeruginosa</i> pro-inflammatory action in CF lungs; particularly, proteases seem to play an important role. Inhibition of virulence factors production and activity resulted in decreased lung inflammation; thus, clarithromycin and protease inhibitors potentially represent additional therapeutic therapies for <i>P. aeruginosa</i>-infected patients.</p

MOESM1 of In vivo monitoring of lung inflammation in CFTR-deficient mice

Additional file 1: Figure S1. In vivo bioluminescence imaging. Monitoring bIL-8 activation in WT and CF transiently transgenized mice with bIL-8-Luc plasmid at 3, 4 and 7 days after DNA delivery. Results are reported photons/sec/cm2 as mean ± SEM, n = 6 each group. Statistical differences were tested by one-way ANOVA followed by Dunnett’s t post hoc test for group comparisons. *p < 0.05 and **p < 0.01