Effect of OSM (10 ng/ml) either alone or in combination with LH (1 ng/ml) on the presence of 3β-HSD positive Leydig cell progenitors

<p><b>Copyright information:</b></p><p>Taken from "Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro"</p><p>http://www.rbej.com/content/5/1/43</p><p>Reproductive biology and endocrinology : RB&E 2007;5():43-43.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2174940.</p><p></p> Interstitial cells were isolated from 13-day-old rat testes and cultured for 1, 3 or 8 days. The presence of 3β-HSD was determined by enzyme histochemistry or immunohistochemistry using a polyclonal antibody against 3β-HSD (for details see Materials and Methods). Statistical analysis revealed that there was no significant difference among the different experiments and thus the results are presented as mean ± SD of three different experiments that were combined. Each single experiment was carried out either in duplicate, triplicate or quadruplicate. Differences among groups were considered to be significantly different when p < 0.05. a – significantly different from control; b – significantly different from OSM 10 ng/ml; c – significantly different from LH 1 ng/ml

Effects of OSM (10 ng/ml) either alone or in combination with LH (1 ng/ml) on the proliferation of interstitial cells isolated from 13-day-old rat testes and cultured for 1, 3 or 8 days

<p><b>Copyright information:</b></p><p>Taken from "Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro"</p><p>http://www.rbej.com/content/5/1/43</p><p>Reproductive biology and endocrinology : RB&E 2007;5():43-43.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2174940.</p><p></p> Proliferation was determined by [H]-thymidine incorporation and scintilation counting (see Materials and Methods). Statistical analysis revealed that there was no significant difference among the different experiments. Therefore, the results are represented as mean ± SD of three different experiments that were combined. Each single experiment was carried out in quadruplicate. Differences among groups were considered to be significantly different when p < 0.05. a – significantly different from control; b – significantly different from OSM 10 ng/ml; c – significantly different from LH 1 ng/ml

Immunohistochemical labelling for the presence of OSM in vivo and in vitro

<p><b>Copyright information:</b></p><p>Taken from "Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro"</p><p>http://www.rbej.com/content/5/1/43</p><p>Reproductive biology and endocrinology : RB&E 2007;5():43-43.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2174940.</p><p></p> A) Section of the testis of a rat 8 days after EDS administration. All Leydig cells have disappeared from the interstitium. Under these conditions no OSM positive cells could be detected by immunohistochemistry; B) Interstitial cell were isolated from testes of 13-day-old rats and cultured for 3 or 8 days without any additions or in the presence of 10 ng/ml OSM and/or 1 ng/ml LH (for more details see Materials and Methods). Percentage of OSM positive cells in culture determined under the indicated culture conditions. The experiment was carried out in quadruplicate. Values are represented as mean ± SD; statistical analysis was carried by comparing the treated cultures with their respective controls. Means were considered to be significantly different at p < 0.05 (indicated by (a)). C) Cells cultured in the presence of LH for 8 days and immunohistochemically stained with an antibody against OSM. Some positively stained cells are indicated by arrows. Scale bar in (A) represents 21 μm, in (C) 20 μm