Modulation of small leucine-rich proteoglycans (SLRPs) expression in the mouse uterus by estradiol and progesterone

<p>Abstract</p> <p>Background</p> <p>We have previously demonstrated that four members of the family of small leucine-rich-proteoglycans (SLRPs) of the extracellular matrix (ECM), named decorin, biglycan, lumican and fibromodulin, are deeply remodeled in mouse uterine tissues along the estrous cycle and early pregnancy. It is known that the combined action of estrogen (E2) and progesterone (P4) orchestrates the estrous cycle and prepares the endometrium for pregnancy, modulating synthesis, deposition and degradation of various molecules. Indeed, we showed that versican, another proteoglycan of the ECM, is under hormonal control in the uterine tissues.</p> <p>Methods</p> <p>E2 and/or medroxiprogesterone acetate (MPA) were used to demonstrate, by real time PCR and immunoperoxidase staining, respectively, their effects on mRNA expression and protein deposition of these SLRPs, in the uterine tissues.</p> <p>Results</p> <p>Decorin and lumican were constitutively expressed and deposited in the ECM in the absence of the ovarian hormones, whereas deposition of biglycan and fibromodulin were abolished from the uterine ECM in the non-treated group. Interestingly, ovariectomy promoted an increase in decorin, lumican and fibromodulin mRNA levels, while biglycan mRNA conspicuously decreased. Hormone replacement with E2 and/or MPA differentially modulates their expression and deposition.</p> <p>Conclusions</p> <p>The patterns of expression of these SLRPs in the uterine tissues were found to be hormone-dependent and uterine compartment-related. These results reinforce the existence of subpopulations of endometrial fibroblasts, localized into distinct functional uterine compartments, resembling the organization into basal and functional layers of the human endometrium.</p

Rapid System to Detect Variants of SARS-CoV-2 in Nasopharyngeal Swabs

Currently, the reference method for identifying the presence of variants of SARS-CoV-2 is whole genome sequencing. Although it is less expensive than in the past, it is still time-consuming, and interpreting the results is difficult, requiring staff with specific skills who are not always available in diagnostic laboratories. The test presented in this study aimed to detect, using traditional real-time PCR, the presence of the main variants described for the spike protein of the SARS-CoV-2 genome. The primers and probes were designed to detect the main deletions that characterize the different variants. The amplification targets were deletions in the S gene: 25-27, 69-70, 241-243, and 157-158. In the ORF1a gene, the deletion 3675-3677 was chosen. Some of these mutations can be considered specific variants, while others can be identified by the simultaneous presence of one or more deletions. We avoided using point mutations in order to improve the speed of the test. Our test can help clinical and medical microbiologists quickly recognize the presence of variants in biological samples (particularly nasopharyngeal swabs). The test can also be used to identify variants of the virus that could potentially be more diffusive as well as not responsive to the vaccine

Influência do manejo do solo e do resíduo da exploração florestal na fauna de solo em plantios híbridos de eucalipto em Mogi Guaçu.

Resumo 31

Determination of electromagnetic medium from the Fresnel surface

We study Maxwell's equations on a 4-manifold where the electromagnetic medium is described by an antisymmetric $2\choose 2$-tensor $\kappa$. In this setting, the Tamm-Rubilar tensor density determines a polynomial surface of fourth order in each cotangent space. This surface is called the Fresnel surface and acts as a generalisation of the light-cone determined by a Lorentz metric; the Fresnel surface parameterises electromagnetic wave-speed as a function of direction. Favaro and Bergamin have recently proven that if $\kappa$ has only a principal part and if the Fresnel surface of $\kappa$ coincides with the light cone for a Lorentz metric $g$, then $\kappa$ is proportional to the Hodge star operator of $g$. That is, under additional assumptions, the Fresnel surface of $\kappa$ determines the conformal class of $\kappa$. The purpose of this paper is twofold. First, we provide a new proof of this result using Gr\"obner bases. Second, we describe a number of cases where the Fresnel surface does not determine the conformal class of the original $2\choose 2$-tensor $\kappa$. For example, if $\kappa$ is invertible we show that $\kappa$ and $\kappa^{-1}$ have the same Fresnel surfaces.Comment: 23 pages, 1 figur

Combined soft and hard X ray ambient pressure photoelectron spectroscopy studies of semiconductor electrolyte interfaces

The development of solar fuel generating materials would greatly benefit from a molecular level understanding of the semiconductor/electrolyte interface and changes in the interface induced by an applied potential and illumination by solar light. Ambient pressure photoelectron spectroscopy techniques with both soft and hard X-rays, AP-XPS and AP-HAXPES respectively, have the potential to markedly contribute to this understanding. In this paper we initially provide two examples of current challenges in solar fuels material development that AP-XPS and AP-HAXPES can directly address. This will be followed by a brief description of the distinguishing and complementary characteristics of soft and hard X-ray AP-XPS and AP-HAXPES and best approaches to achieving monolayer sensitivity in solid/aqueous electrolyte studies. In particular we focus on the detection of surface adsorbed hydroxyl groups in the presence of aqueous hydroxide anions in the electrolyte, a common situation when investigating photoanodes for solar fuel generating applications. The paper concludes by providing an example of a combined AP-XPS and AP-HAXPES study of a semiconductor/aqueous electrolyte interface currently used in water splitting devices specifically the BiVO4/aqueous potassium phosphate electrolyte interface

Chemical, Structural, and Electronic Characterization of the 010 Surface of Single Crystalline Bismuth Vanadate

We have structurally, chemically and electronically characterized the most stable 010 surface of a Mo doped BiVO4 single crystal. Low energy electron diffraction LEED reveals that the surface is not significantly reconstructed from a bulk termination of the crystal. Synchrotron based X ray spectroscopies indicate no surface enhancement of any of the crystal constituents and that the Mo dopant occupies tetrahedral sites by substituting for V at the surface. Using resonant photoemission to study the valence band structure as the V L3 edge is scanned we observe an intra band gap state associated with reduced vanadium formed by the Mo doping. This state is likely associated with small polaron formation at the surface. This feature is enhanced at a photon energy that is not resonant with any of the main features in the absorption spectrum of the pristine BiVO4. This indicates that the additional electron from Mo doping likely induces further distortion of the VO4 tetrahedral units and generates a new conduction band state either by splitting of the V dz2 states or by hybridization of V dzx and V dz2 states. We measure a work function of 5.15 eV for the BiVO4 010 surface. Measurement of the work function allows us to recast the electronic energy levels onto the normal hydrogen electrode scale for comparison to the standard reduction and oxidation potentials of water. This detailed study should provide a basis for future work aimed at a molecular level understanding of BiVO4 electrolyte interfaces used for photoelectrochemical water splittin

Efeito do manejo do solo e do resíduo da exploração florestal na diversidade da fauna de solo em plantios de Eucalyptus grandis, em São Miguel Aracanjo, SP.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso