798 research outputs found

    Phytochemical composition and antioxidant activity of Lavandula dentate extracts

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    Le but de ce travail consiste à étudier la composition des huiles essentielles et des polyphénols des racines, des tiges et des feuilles de la Lavande dentée et d’évaluer leurs potentialités antioxydantes. L’analyse et la quantification des huiles essentielles a montré que les feuilles sont les plus riches en huiles essentielles (0.89 mg/g MS) suivies par les tiges (0.68 mg/g MS) et enfin les racines (0,23 mg/g MS). Le constituant majeur de l’HE des racines est: le β-ocimène. D’autre part, le limonène représente le composé majeur de l’HE des tiges. Quant à l’HE des feuilles, elle est dominée par le camphre. D’autre part, nos résultats ont montré que les organes de la lavande montrent des teneurs en polyphénols totaux élevées et variables selon l’organe étudié. En effet, les extraits des racines sont caractérisés par le contenu le plus élevé en polyphénols. D’autre part, l’étude de l’activité antioxydante des extraits des différents organes a indiqué que les extraits de la racine sont particulièrement les plus actifs et que leur analyse par RP-HPLC a montré que ces derniers sont riches essentiellement en acide rosmarinique. Finalement, les extraits de la Lavande dentée et particulièrement ceux de la racine peuvent être considérés comme des sources alternatives d’antioxydants naturels puissants qui peuvent être utilisés en industrie agroalimentaire et pharmaceutique.In this study, Lavandula dentata organs (roots, stems and leaves) were investigated for their essential oils, total phenolics, flavonoids contents and antioxidant activities. Essential oil yields were 0.22% in roots, 0.68 % in stems and 0.89 % in flowers. Major components of the oils were β-ocimene, limonene and 1,8 cineol in roots, stems and leaves and flowers, respectively. In all organs, total phenolics content ranged from 42.57 to 16.17 mg gallic acid equivalents per gram of dry weight (mg GAE/g DW).The antioxidant activities of Lavandula dentata extracts obtained from the three organs were assessed using two tests (DPPH and reducing power). The root extract was strongly effective as DPPH radical scavenger and reducing agent. Thus, the identification of individual target polyphenolic compounds of roots was performed by RP-HPLC. The major phenolic compound detected in roots was rosmarinic acid. This activity was high enough for the plant to be a new and natural source of strongly antioxidant substances for use as natural additives in food and pharmaceutical industry

    Chemical Composition and Antioxidant Activity of seeds oils and fruit juice of Opuntia Ficus Indica and Opuntia Dillenii from Morocco

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    peer reviewedThis study provides basic information on the mineral composition of the seeds and antioxidant activity in seeds oils and fruit juices of cactus belonging to two species Opuntia ficus indica and Opuntia dillenii, from Morocco (Oujda), in order to evaluate the nutritional value of the Opuntia extracts. Minerals determined from dry seeds of Opuntia ficus indica and Opuntia dillenii were: calcium 480.93 and 408.28; phosphorus 1417.59 and 970.15; potassium 304.51 and 201.96; magnesium: 316.59 and 240.30; sodium: 48.33 and 18.18; zinc: 70.77 and 78.26 mg/100g respectively. The main fatty acids of Opuntia ficus indica and Opuntia dillenii seed oil were respectively: linoleic acid: 58.79 and 79.83%, Palmitic acid: 11.18 and 13.52%. The antioxidant activity of Opuntia ficus indica and Opuntia dillenii seed oils and fruit juices were assessed by means of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and ascorbic acid test. The results showed that the antioxidant activities of Opuntia ficus indica and Opuntia dillenii seed oil (IC50 = 19.79 ± 0.023 and 27.21 ± 0.075 μL/mL) are higher than that of the reference ascorbic acid (IC50 = 16.56 ± 0.019 μg/mL). However, the Opuntia dillenii juice presents antioxidant activity more important than this of Opuntia seed oil and ascorbic acid. It possessed strong antioxidant activity (IC50 = 8.18 μL/mL). The antioxidant activity of the seed oil and juice were also found to be concentration-dependent

    Variability in almond oil chemical traits from traditional cultivars from eastern Morocco

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    In Morocco, cultivation of almond tree (Prunus amygdalus L.) constitutes the second most important plantation of fruit trees after olive growing. It is mostly cultivated in two regions, « Taza, Al Houceima Taounate » in the north and « Souss Massa Draa » in the south. Almond genetic resources (Marcoma, Fournat, Ferragnes/Ferraduel and Beldi), cultivated in eastern Morocco were studied during two consecutive crop years in order to evaluate variations in kernel oil yield, fatty acid profiles, oleic /linoleic (O/L) ratio and almond oils oxydative stability (OSI,evaluated by rancimat tests) in comparison to monovarietal olive oils. Almond kernel total oil (AO), Oleic acid (C18:1), Linoleic acid (C18:2), O/L-ratio, and tocopherol contents range between: 48 - 62% for kernel total oil; 65- 77.5% for C18:1; 17- 25% for C18:2; 2.5-4 for O/L ratio and 370 - 675 μg/g oil for tocopherols, respectively. We conclude that the genotype is the main variability source for all these chemical traits of AOs. Results obtained from Ferragnes/Ferraduel may be of interest for almond breeding focused to improve kernel oil yield and fatty acid profile. Besides, tocopherols contents of AOs seem to be the most important contributor for their stability to oxidation, even though compared to monovarietal olive oils, stability of AOs were very low and OSI value range between 20-27 hours. This fragility of AOs is due to their high content of unsaturated fatty acid which not allows their use for cooking or storage for long period. However, almond oils could have many applications in the food industry as in cosmeti

    Assessment of fatty acids profile, oil yield and tocopherol content of four Almond cultivars grown in Eastern Morocco

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    The most cultivated varieties of almond in eastern Morocco: (Beldi (B), a local ecotype, Marcona (M) from Spain, Ferragnes-Ferraduel (F-F) and Fournat de Breznaud (FNB) from France), were studied during three consecutive crop years in order to evaluate variations in kernel oil yield, Fatty acid (FA) profile and physicochemical properties. For this purpose, extraction of almond oils was carried out by mechanical press. The yield of varieties B, M, (F-F) and FNB ranged between 50.68%- 54.33%, 41.46%- 52.59%, 47.70%-52.39% and 51.66%-56.10%, respectively. Oleic, linoleic and palmitic acids are the major fatty acids (FA) ranging between 57.54%- 72.90%, 17.80%- 29.81% and 6.50%-8.48%, respectively. Results showed a noticeable effect (P<0.001) of variety on Total phenolic content (TPC), oxidative stability and α-, β-, γ-, δ-tocopherol isomers; however, acidity and peroxide index, were affected with a lower manner by "variety" factor. In addition, all the analyzed parameters were highly (P<0.001) affected by climatic conditions of the crop year. In addition, the highest variations for the analyzed almond oils were recorded for their contents on α-tocopherol, γ-tocopherol, oleic and linoleic acids. According to the observed results, the couple Ferragnes-Ferraduel seems to produce stable and high quality almond oil compared to the other varieties

    Cell Microbiol.

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    Helicobacter pylori is one of the most common bacterial pathogens, infecting about 50% of the world population. The presence of a pathogenicity island (PAl) in H. pylori has been associated with gastric disease. We present evidence that the H. pylori protein encoded by the cytotoxin- associated gene A (cagA) is translocated and phosphorylated in infected epithelial cells. Two-dimensional gel electrophoresis (2-DE) of proteins isolated from infected AGS cells revealed H. pylori strain-specific and time- dependent tyrosine phosphorylation and dephosphorylation of several 125-135 kDa and 75-80 kDa proteins. Immunoblotting studies, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), cell fractionation and confocal microscopy demonstrated that one of the 125-135 kDa proteins represents the H. pylori CagA protein, which is translocated into the host cell membrane and the cytoplasm. Translocation of CagA was dependent on functional cagA gene and virulence (vir) genes of a type IV secretion apparatus composed of virB4, virB7, virB10, virB11 and virD4 encoded in the cag PAl of H. pylori. Our findings support the view that H. pylori actively translocates virulence determinants, including CagA, which could be involved in the development of a variety of gastric disease.SCOPUS: ar.jFLWINinfo:eu-repo/semantics/publishe

    Origin of symbol-using systems: speech, but not sign, without the semantic urge

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    Natural language—spoken and signed—is a multichannel phenomenon, involving facial and body expression, and voice and visual intonation that is often used in the service of a social urge to communicate meaning. Given that iconicity seems easier and less abstract than making arbitrary connections between sound and meaning, iconicity and gesture have often been invoked in the origin of language alongside the urge to convey meaning. To get a fresh perspective, we critically distinguish the origin of a system capable of evolution from the subsequent evolution that system becomes capable of. Human language arose on a substrate of a system already capable of Darwinian evolution; the genetically supported uniquely human ability to learn a language reflects a key contact point between Darwinian evolution and language. Though implemented in brains generated by DNA symbols coding for protein meaning, the second higher-level symbol-using system of language now operates in a world mostly decoupled from Darwinian evolutionary constraints. Examination of Darwinian evolution of vocal learning in other animals suggests that the initial fixation of a key prerequisite to language into the human genome may actually have required initially side-stepping not only iconicity, but the urge to mean itself. If sign languages came later, they would not have faced this constraint
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