Behavior of Melanoma Cells in Cell and Organ Cultures: Use of Biomaterials to Activate Cells

It is well known that cell behaviors such as adhesion, proliferation and various synthesis are initiated from transmembrane signals. This study uses biomaterials as primary messengers of the cell activation pathways, and we have analyzed the effects of two biomaterials on highly metastatic tumor cells. B16F10 melanoma cells formed heterogeneous populations whose size varied with cell differentiation. In long-term organ cultures grown comparatively on AN 69 and Cuprophan (a biomembrane known to activate cells), we found that Cuprophan increased both adhesion and proliferation of small melanin-rich cells which represented differentiated melanocyte&. In dissociated cell cultures, the rate of early cell attachment decreased on Cupropban compared to AN 69 and control Thermanox® (Nunc Inc., Naperville, IL). Scanning electron microscopy of melanocytes four hoUl11 after plating out on Cuprophan revealed only cell aggregates, comparable to the 3T3 fibroblasts aggregates previously described. Nevertheless, the production of the second messenger cyclic adenosine monophosphate (cAMP) was the same on both materials, in contrast to previous results showing more cAMP in cells on Cuprophan. Therefore, biomaterials appear to be useful tools for investigating as well attachment, growth, differentiation as signal transduction pathways of cancerous cells

Fundamental limitations for quantum and nano thermodynamics

The relationship between thermodynamics and statistical physics is valid in the thermodynamic limit - when the number of particles becomes very large. Here, we study thermodynamics in the opposite regime - at both the nano scale, and when quantum effects become important. Applying results from quantum information theory we construct a theory of thermodynamics in these limits. We derive general criteria for thermodynamical state transformations, and as special cases, find two free energies: one that quantifies the deterministically extractable work from a small system in contact with a heat bath, and the other that quantifies the reverse process. We find that there are fundamental limitations on work extraction from nonequilibrium states, owing to finite size effects and quantum coherences. This implies that thermodynamical transitions are generically irreversible at this scale. As one application of these methods, we analyse the efficiency of small heat engines and find that they are irreversible during the adiabatic stages of the cycle.Comment: Final, published versio

Myiasis in domestic cats: A global review

Myiasis is an infestation caused by larvae of Diptera in humans and other vertebrates. In domestic cats, Felis silvestris catus L. (Carnivora: Felidae), four dipteran families have been reported as agents of obligatory and facultative myiasis: Oestridae, Calliphoridae, Sarcophagidae and Muscidae. Among agents of obligatory myiasis, the most frequent genus is Cuterebra Clark (Oestridae) and the most frequent species is Cochliomyia hominivorax (Coquerel) (Calliphoridae). Among the agents of facultative myiasis, the most frequent species is Lucilia sericata (Meigen) (Calliphoridae). A survey of myiasis in cats reported in literature shows that the cases are distributed worldwide and linked to the geographical range of the dipteran species. Factors favouring the occurrence of myiasis in cats are prowling in infested areas, poor hygiene conditions due to diseases and/or neglect, and wounds inflicted during territorial or reproductive competition. The aim of the review is to provide an extended survey of literature on myiasis in cats, as general information and possible development of guidelines for veterinarians, entomologists and other researchers interested in the field

Toward physical realizations of thermodynamic resource theories

Conventional statistical mechanics describes large systems and averages over many particles or over many trials. But work, heat, and entropy impact the small scales that experimentalists can increasingly control, e.g., in single-molecule experiments. The statistical mechanics of small scales has been quantified with two toolkits developed in quantum information theory: resource theories and one-shot information theory. The field has boomed recently, but the theorems amassed have hardly impacted experiments. Can thermodynamic resource theories be realized experimentally? Via what steps can we shift the theory toward physical realizations? Should we care? I present eleven opportunities in physically realizing thermodynamic resource theories.Comment: Publication information added. Cosmetic change

Hydrodynamic Coupling of Two Brownian Spheres to a Planar Surface

We describe direct imaging measurements of the collective and relative diffusion of two colloidal spheres near a flat plate. The bounding surface modifies the spheres' dynamics, even at separations of tens of radii. This behavior is captured by a stokeslet analysis of fluid flow driven by the spheres' and wall's no-slip boundary conditions. In particular, this analysis reveals surprising asymmetry in the normal modes for pair diffusion near a flat surface.Comment: 4 pages, 4 figure

Stokes' Drift of linear Defects

A linear defect, viz. an elastic string, diffusing on a planar substrate traversed by a travelling wave experiences a drag known as Stokes' drift. In the limit of an infinitely long string, such a mechanism is shown to be characterized by a sharp threshold that depends on the wave parameters, the string damping constant and the substrate temperature. Moreover, the onset of the Stokes' drift is signaled by an excess diffusion of the string center of mass, while the dispersion of the drifting string around its center of mass may grow anomalous.Comment: 14 pages, no figures, to be published in Phys.Rev.

Feynman's ratchet and pawl: an exactly solvable model

We introduce a simple, discrete model of Feynman's ratchet and pawl, operating between two heat reservoirs. We solve exactly for the steady-state directed motion and heat flows produced, first in the absence and then in the presence of an external load. We show that the model can act both as a heat engine and as a refrigerator. We finally investigate the behavior of the system near equilibrium, and use our model to confirm general predictions based on linear response theory.Comment: 19 pages + 10 figures; somewhat tighter presentatio

Effect of Chaotic Noise on Multistable Systems

In a recent letter [Phys.Rev.Lett. {\bf 30}, 3269 (1995), chao-dyn/9510011], we reported that a macroscopic chaotic determinism emerges in a multistable system: the unidirectional motion of a dissipative particle subject to an apparently symmetric chaotic noise occurs even if the particle is in a spatially symmetric potential. In this paper, we study the global dynamics of a dissipative particle by investigating the barrier crossing probability of the particle between two basins of the multistable potential. We derive analytically an expression of the barrier crossing probability of the particle subject to a chaotic noise generated by a general piecewise linear map. We also show that the obtained analytical barrier crossing probability is applicable to a chaotic noise generated not only by a piecewise linear map with a uniform invariant density but also by a non-piecewise linear map with non-uniform invariant density. We claim, from the viewpoint of the noise induced motion in a multistable system, that chaotic noise is a first realization of the effect of {\em dynamical asymmetry} of general noise which induces the symmetry breaking dynamics.Comment: 14 pages, 9 figures, to appear in Phys.Rev.

Direct measurements of the effects of salt and surfactant on interaction forces between colloidal particles at water-oil interfaces

The forces between colloidal particles at a decane-water interface, in the presence of low concentrations of a monovalent salt (NaCl) and of the surfactant sodium dodecylsulfate (SDS) in the aqueous subphase, have been studied using laser tweezers. In the absence of electrolyte and surfactant, particle interactions exhibit a long-range repulsion, yet the variation of the interaction for different particle pairs is found to be considerable. Averaging over several particle pairs was hence found to be necessary to obtain reliable assessment of the effects of salt and surfactant. It has previously been suggested that the repulsion is consistent with electrostatic interactions between a small number of dissociated charges in the oil phase, leading to a decay with distance to the power -4 and an absence of any effect of electrolyte concentration. However, the present work demonstrates that increasing the electrolyte concentration does yield, on average, a reduction of the magnitude of the interaction force with electrolyte concentration. This implies that charges on the water side also contribute significantly to the electrostatic interactions. An increase in the concentration of SDS leads to a similar decrease of the interaction force. Moreover the repulsion at fixed SDS concentrations decreases over longer times. Finally, measurements of three-body interactions provide insight into the anisotropic nature of the interactions. The unique time-dependent and anisotropic interactions between particles at the oil-water interface allow tailoring of the aggregation kinetics and structure of the suspension structure.Comment: Submitted to Langmui

Molecular motor that never steps backwards

We investigate the dynamics of a classical particle in a one-dimensional two-wave potential composed of two periodic potentials, that are time-independent and of the same amplitude and periodicity. One of the periodic potentials is externally driven and performs a translational motion with respect to the other. It is shown that if one of the potentials is of the ratchet type, translation of the potential in a given direction leads to motion of the particle in the same direction, whereas translation in the opposite direction leaves the particle localized at its original location. Moreover, even if the translation is random, but still has a finite velocity, an efficient directed transport of the particle occurs.Comment: 4 pages, 5 figures, Phys. Rev. Lett. (in print