Analisis Kandungan Akrilamida Dalam Pisang Goreng Yang Beredar Di Kota Manado Menggunakan Kromatografi Cair Kinerja Tinggi (KCKT)

Acrylamide can be formed as a consequence of heating athigh temperature (more than1200C) in food contains high of carbohydrate. Acrylamide was classified as carcinogeniccompound to human being. Junk food which mostly eaten by people is fries. The objectiveof this research is to identify acrylamide in banana fries marketed in Manado city usingHigh Performance Liquid Chromatography (HPLC). Samples were taken from 12 places,by 6 sub district Manado City. Acrylamide were analyzed by HPLC using Shim-Pack VPODS(4,6 x 250 nm) column, comparison of mobile phase acetonitrile and phospate acid11,45 mM (5:95), flow rate 1 ml/minuteand at wave lenght 210 nm. The result shows thatAcrylamide contains in banana fries with retention time ±4,284 minutes. Acrylamidecontained in banana fries which marketed in Manado City were 39,65 – 1789,52 μg/kg.Acrylamide level in banana fries is at under lethal dose (LD50

Uji Daya Hambat Ekstrak Rimpang Kunyit (Curcuma Longa) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Pseudomonas SP.

: Turmeric (Curcuma longa) is a plant that is known to have medicinal properties, especially the rhizome. The active compound that contained in the rhizomes are able to work as an antibacterial. This study aimed to measure the inhibitory of turmeric rhizome (Curcuma longa) extract against the growth of Staphylococcus aureus and Pseudomonas sp. This was an experimental laboratory study. The polar extract of turmeric rhizome (Curcuma longa) was tested by well method with concentration of 40%, 20%, 10%, and 5%. The result showed that the polar extract of turmeric rhizome (Curcuma longa) with a concentration of 40%, 20%, 10%, 5% can inhibit the growth of Staphylococcus aureus with the average of each is 15,0 mm, 14,5 mm, 13,5 mm and 11,0 mm while Pseudomonas sp with a mean diameter of each is 13,1 mm, 11,1 mm, 9,3 mm and 8,8 mm. Conclusion: The polar extract of turmeric rhizome (Curcuma longa) has an inhibitory effect against the growth of Staphylococcus aureus and Pseudomonas sp

Pengaruh Pemberian Ekstrak Etanol Daun Paku Sisik Naga (Drymoglossum Piloselloides L.presl) Terhadap Peroksidasi Lipid Hati Pada Tikus Jantan Galur Wistar Yang Diinduksi Ccl4

The aims of this research was to determine the effect of ethanol extract paku sisik nagaleaves against lipid peroxidation in male wistar liver induced by CCl4. Method of thisresearch used laboratory experiment using 9 white male wistar were divided into 3 treatmentgroups. All groups were given treatment during ten days. The first group (negative control)was given distilled water ad libitum, the second group was given ethanol extract of paku sisiknaga leaves 97.02 mg/kg body weight per oral and the third group (positive control) wasgiven Curcuma 45 mg/kg body weight per oral. On the tenth day, after two hours oftreatment all groups were given CCl4 1mL/kg body weight intraperitonial, eighteen hoursafter that rats in all groups were dissected and taken his liver. Then rat liver analyzed byTBARS method (Thiobarbiturate Acid Reactive Subtance) to measure the levels of MDA(Malondialdehyde) which is an indicator of lipid peroxidation in the rat liver. Data wereanalyzed by SPSS ver.20, significant difference between treatments was tested by one-wayANOVA. The results showed a significant difference with 95% confidence level (α = 0.05)with a mean value of MDA 3,828 μM negative control group, group of ethanol extract ofpaku sisik naga leaf was 2,666 μM and 2,211 μM for positive control group. Ethanol extractof paku sisik naga leaves with dose 97.02 mg/kg body weight can prevent the process of lipidperoxidation in rat liver

Identifikasi Bakteri Resisten Merkuri Dalam Urine, Feses, Dan Karang Gigi Pada Individu Di Daerah Pesisir Pantai Desa Pulisan Kecamatan Likupang Timur Kabupaten Minahasa Utara

: Mercury is a metal that is very harmful for health human and the environment if not properly mercury polluted sea water can be exposed to humans especially those living in coastal areas with high consumption of marine products. Mercury pollution in the ocean triggered a mercury-resistant bacteria therefore researchers are interested in finding out if the bacteria are resistant to mercury in urine, feces, and tartar. Research design is fashion descriptive explorative. Samples taken in this study is urine, feces, and tartar on individuals who have settled more than 30 years in the rural coastal districts Pulisan, Likupang eastern district north Minahasa regency then tested in morphology, physiology and biochemistry at the FMIPA Sam Ratulangi University Sam Ratulangi biotechnology lab. Result of the study showed the presence of mercury-resistant bacteria in samples taken were found proved by mercury resistant bacteria 4 of 6 isolates that survive up to 40 ppm and 20 ppm in HgCl2 on Phenyl mercury is Bacillus sp, E.coli, Streptococcus sp, and Staphylococcus sp

Studi Karakteristik Dan Penggunaan Obat Pada Penderita Hepatitis B Di Rumah Sakit Pemerintah Kota Manado Periode Januari 2011 – Desember 2012

Hepatitis B is a disease caused by the virus called hepatitis B wich characterized by aninflammation that occurs in organs such as the liver. The transmission of Hepatitis B is higherthan the transmission of the HIV virus. This study aimed to investigate the characteristics anduse of the drug in hepatitis B patients in the Government Hospital in Manado. This researchis a descriptive study based on retrospective data collection for 2 years survey from January2011 to December 2012. The respondent of this research involve of 70 patients with HepatitisB medical record. The results showed that based on group age, responden with age group 46-65 has the highest percentage by 31 patients (44.3%), based on gender, males have thehighest percentage of total of 47 patients (67.1%), based on the duration of treatment, thehighest percentage is shown by the patient with 1 day threatment as 21 patients (30%). Basedon therapeutic treatment this research divided it into 2 kind of threatment. The first us singleagent therapy Hepamax consist of 27 patients (38.6%) The second therapy is combinationbetween Hepamax and Curcuma wich consist of 9 patients (12.9%). This research conductedbased on 100% oral route of medicine threatment

Uji Resistensi Bakteri Bacillus SP Yang Diisolasi Dari Plak Gigi Terhadap Merkuri Dan Eritromisin

: Mercury is a toxic heavy metal that is used for dental fillings in the form of amalgam. To reduce the toxic effects produced by mercury, mercury resistant bacteria can be used. Bacillus sp is a gram-positive bacteria that is resistant to mercury. Besides having the mer operon genes that can transform Hg2+ to Hg0 that is less toxic, Bacillus sp bacteria also produce esterase that cause these bacteria resistant to erythromycin antibiotic. Erythromycin is a macrolide class of antibiotic used for the treatment of diseases caused by Gram-positive bacteria, especially Staphylococcus and Diphtheroids. To determine the resistance of Bacillus sp bacteria against mercury and erythromycin antibiotic. This study used a descriptive exploratory method with samples of bacteria and mercury are already available in the Laboratory of Pharmaceutical Microbiology. Based on the research that has been conducted, showed that the Bacillus sp bacteria is resistant to mercury and erythromycin

Identifikasi Dan Penetapan Kalium Iodat Dalam Garam Dapur Yang Beredar Di Pasar Kota Bitung Dengan Metode Spektrofotometri Uv-vis

Iodine deficiency disorder can be prevented by consumption of salt which contains iodine into the body. Based on Indonesian National Standard (SNI), concentration of Potassium iodide(KIO3) were 30-80 ppm. The objectives of this research were to identify and determineconcentration of potassium iodide in salt which marketed in Bitung region. Samples were 9branded salts and 1 sample unbranded. Identification was done by Potassium cation test andIodide anion test. Determination of Potassium iodide were done using spectrophotometerUV-Vis. The results shows that 9 samples contains Potassium iodide (Branded salts), and 1sample did not contains Potassium iodide (Unbranded salt). Based on spectrophotometeranalysis, there were 5 samples comply SNI of KIO3 (31,6-77,6 ppm), 5 samples did notcomply SNI of KIO3 (2,1-28 ppm)