Barking up the right tree: Understanding local attitudes towards dogs in villages surrounding Ranomafana National Park, Madagascar can benefit applied conservation

Exotic carnivores, particularly feral and domestic dogs, represent a serious threat to Madagascar’s endemic fauna. We obtained information from the local community about dogs in villages in and around Ranomafana National Park (RNP), Madagascar. Surveys were conducted (N=359) to assess local opinions of dogs, reasons for owning dogs, and the willingness of dog owners to participate in spay/neuter/vaccine programs. Of surveyed individuals without dogs (N=211), 58.9% of respondents reported negative feelings towards free-roaming dogs, with only 1% of respondents identifying free-roaming dogs as a positive aspect of village life. Of individuals with dogs (N=148), 8.1% of respondents reported using their dog for hunting, and 41.2% reported that their dog had killed at least one wild animal, with 11.8% reporting that this occurred on a weekly basis. Villagers approve of spay/neuter/vaccine programs and 90.3% of respondents with dogs state they would use them if freely available. The interest in veterinary services combined with a generally negative attitude towards free-roaming dogs indicates that a spay/neuter/vaccine program would be an effective means of controlling dog populations.RÉSUMÉLes carnivores exotiques, particulièrement les chiens domestiques et ceux retournés à l’état sauvage, représentent une menace sérieuse pour la faune endémique de Madagascar. Nous avons récolté des informations auprès des communautés riveraines sur les chiens vivant dans les villages et autour du Parc National de Ranomafana (RNP) au sud-est de Madagascar. Nous avons mené des enquêtes (N=359) afin d’évaluer les avis de la communauté locale sur les chiens, les raisons pour lesquelles les gens possèdent ces animaux et la volonté des propriétaires pour s’engager dans un programme de stérilisation/vaccination canine. Les villageois qui ne possédaient pas de chiens (N=211) représentaient 58,9 % des personnes interrogées ; ils ont rapporté avoir des sentiments négatifs envers les chiens errants et seulement 1 % des personnes interrogées ont vu un aspect positif pour la vie du village dans les chiens errants. Parmi les propriétaires de chiens (N=148), 8,1 % des personnes interrogées ont rapporté utiliser leur chien pour la chasse et 41,2% des personnes interrogées indiquent que leur chien a déjà tué au moins un animal sauvage, dont 11,8 % rapportant que cela arrivait toutes les semaines. Les villageois approuvent le programme de stérilisation/ vaccination canine et 90,3 % des propriétaires de chiens y auraient volontiers recours si celui-ci était gratuit et librement disponible

Dietary macronutrient composition impacts gene regulation in adipose tissue

Diet is a key lifestyle component that influences metabolic health through several factors, including total energy intake and macronutrient composition. While the impact of caloric intake on gene expression and physiological phenomena in various tissues is well described, the influence of dietary macronutrient composition on these parameters is less well studied. Here, we use the Nutritional Geometry framework to investigate the role of macronutrient composition on metabolic function and gene regulation in adipose tissue. Using ten isocaloric diets that vary systematically in their proportion of energy from fat, protein, and carbohydrates, we find that gene expression and splicing are highly responsive to macronutrient composition, with distinct sets of genes regulated by different macronutrient interactions. Specifically, the expression of many genes associated with Bardet-Biedl syndrome is responsive to dietary fat content. Splicing and expression changes occur in largely separate gene sets, highlighting distinct mechanisms by which dietary composition influences the transcriptome and emphasizing the importance of considering splicing changes to more fully capture the gene regulation response to environmental changes such as diet. Our study provides insight into the gene regulation plasticity of adipose tissue in response to macronutrient composition, beyond the already well-characterized response to caloric intake

Measuring the Impact of Targeting FcRn-Mediated IgG Recycling on Donor-Specific Alloantibodies in a Sensitized NHP Model

Background: In transplantation, plasmapheresis and IVIg provide the mainstay of treatment directed at reducing or removing circulating donor-specific antibody (DSA), yet both have limitations. We sought to test the efficacy of targeting the IgG recycling mechanism of the neonatal Fc receptor (FcRn) using anti-FcRn mAb therapy in a sensitized non-human primate (NHP) model, as a pharmacological means of lowering DSA. Methods: Six (6) rhesus macaque monkeys, previously sensitized by skin transplantation, received a single dose of 30mg/kg anti-RhFcRn IV, and effects on total IgG, as well as DSA IgG, were measured, in addition to IgM and protective immunity. Subsequently, 60mg/kg IV was given in the setting of kidney transplantation from skin graft donors. Kidney transplant recipients received RhATG, and tacrolimus, MMF, and steroid for maintenance immunosuppression. Results: Circulating total IgG was reduced from a baseline 100% on D0 to 32.0% (mean, SD +/- 10.6) on d4 post infusion (p\u3c0.05), while using a DSA assay. T-cell flow cross match (TFXM) was reduced to 40.6+/-12.5% of baseline, and B-cell FXCM to 52.2+/-19.3%. Circulating total IgM and DSA IgM were unaffected by treatment. Pathogen-specific antibodies (anti-gB and anti-tetanus toxin IgG) were significantly reduced for 14d post infusion. Post-transplant, circulating IgG responded to anti-FcRn mAb treatment, but DSA increased rapidly. Conclusion: Targeting the FcRn-mediated recycling of IgG is an effective means of lowering circulating donor-specific IgG in the sensitized recipient, although in the setting of organ transplantation mechanisms of rapid antibody rise post-transplant remains unaffected