Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells as an Alternative Cell Source for Cell-based Osteoarthritis Treatment: A Systematic Review on Preclinical and Clinical Evidence

ABSTRACT Introduction: There is still no definitive treatment to either inhibit the degradation process or restore the damaged cartilage in osteoarthritis (OA). Various cell sources have been studied and in vitro studies showed that infrapatellar fat pad-derived mesenchymal stem cells (IFPDMSCs) exhibit higher chondrogenic potential than other adipose-derived cells. Still, very few in vivo studies on IFPDMSCs for cartilage healing in OA have been reported. Aims: This systematic review will analyze the therapeutic potential of IFPDMSCs for cartilage healing in osteoarthritis from preclinical and clinical studies. Design, Methods, and Data Source: Using the PubMed, EMBASE, and Cochrane Library database up to November 30, 2020, a systematic review according to PRISMA reporting guideline was conducted on IFPDMSCs application to treat osteoarthritis in vivo studies. Inclusion criteria were in vivo preclinical and clinical studies from January 2010 to November 2020 involving the OA model or cases using IFPDMSCs to promote healing. Results: In vivo studies are scarce. Only four studies are included: two animals and two clinical studies. All included studies demonstrate favourable results of IFPDMSCs in osteoarthritis, but there is heterogeneity in outcome measurement among all studies. Conclusion: The in vitro and currently limited in vivo studies showed that infrapatellar fat pad-derived mesenchymal stem cells offer an alternative cell source with promising chondrogenic healing potential. Impact: More preclinical and clinical in vivo studies should be encouraged to explore and support the efficacy of IFPDMSCs in cell-based OA treatment to prove the promising result as those of the in vitro studies

EFEKTIVITAS NANOEMULSI SEBAGAI PENGHANTAR UBIQUINONE (Q10) DIBANDINGKAN DENGAN EMULSI PADA SEDIAAN KOSMETIKA ANTI AGING (Menggunakan Fase Minyak Olive Oil)

Ubiquinone (Q10) is an endogenous antioxidant. It is lipophilic and practically insoluble in water. To improve its solubility and penetration, it was formulated into O/W nanoemulsion. The resulting Q10 nanoemulsion was then physically characterized for its viscosity, surface tension, morphology, droplet size, and polydispersity index. In addition, it was conducted a penetration test of Q10 and effectiveness Q10 as anti aging in nanoemulsion delivery system compared to Q10 in emulsion using male Wistar rats. From the characterization results, that the viscosity of Q10 nanoemulsion (10.5 CPas) < Q10 emulsion (16 CPas), the morphology of droplet Q10 nanoemulsion and Q10 emulsion that are spherical, and it was found that the droplet size of Q10 nanoemulsion (70.07 ± 12.42 nm) < Q10 emulsion (21.063 ± 3,57μm), The penetration rate of Q10 in nanoemulsion and emulsion was observed at 2 hours, 4 hours, and 6 hours after treatment. The result was known that the penetration rate of Q10 in nanoemulsion (174.49 μm/h) > Q10 in emulsion (20.429 μm/h). Effectivness as anti aging using the number of fibroblast and collagen density. The number of fibroblasts were obtained in Q10 nanoemulsion (39 ± 6,04) > Q10 emulsion (23 ± 2,07). Collagen density test results on Q10 nanoemulsi (1.8 ± 0.44)> Q10 emulsion (1.6 ± 0.54)

Hubungan Antara Tingkat Pengetahuan dan Sikap Dalam Pemilihan Produk Moisturizer pada Masyarakat Malang Raya.

Moisturizer atau pelembab wajah merupakan produk perawatan kulit yang berfungsi untuk menghidrasi kulit. Moisturizer merupakan produk perawatan kulit kedua yang paling banyak digunakan oleh wanita Indonesia setelah produk sabun pencuci muka. Moisturizer memiliki berbagai kandungan dan bentuk sediaan. Apabila dalam pemilihan moisturizer tidak dilakukan dengan sikap yang tepat maka moisturizer tidak akan memberikan manfaat terbaik bahkan dapat menimbulkan efek samping. Tujuan penelitian ini yaitu untuk mengetahui adanya hubungan antara tingkat pengetahuan dan sikap dalam pemilihan produk moisturizer pada masyarakat Malang Raya. Desain penelitian yang digunakan yaitu observasional analitik dengan metode cross sectional. Sampel yang digunakan yaitu masyarakat Malang Raya (Kabupaten Malang, Kota Malang, dan Kota Batu) sebanyak 100 responden dengan teknik purposive sampling yang sesuai dengan kriteria inklusi dan eksklusi. Instrumen yang digunakan adalah kuesioner untuk mengukur tingkat pengetahuan dan sikap dalam pemilihan produk moisturizer dan telah diuji validitas dan reliabilitasnya. Hasil penelitian ini adalah masyarakat Malang Raya memiliki tingkat pengetahuan baik (32%), cukup (51%), dan kurang (17%) sedangkan yang memiliki sikap positif (41%), dan sikap negatif (59%). Hasil uji spearman menunjukkan nilai signifikansi 0,007 (p < 0,05) dan nilai korelasi sebesar +0,267, hal tersebut menandakan bahwa terdapat hubungan yang signifikan dan tingkat korelasi yang sedang. Oleh karena itu, diperlukannya peran apoteker untuk mengedukasi masyarakat dalam pemilihan dan penggunaan moisturizer dengan tepat

Infrapatellar Fat Pad-Derived Mesenchymal Stem Cells as an Alternative Cell Source for Cell-based Osteoarthritis Treatment: A Systematic Review on Preclinical and Clinical Evidence

ABSTRACT Introduction: There is still no definitive treatment to either inhibit the degradation process or restore the damaged cartilage in osteoarthritis (OA). Various cell sources have been studied and in vitro studies showed that infrapatellar fat pad-derived mesenchymal stem cells (IFPDMSCs) exhibit higher chondrogenic potential than other adipose-de�rived cells. Still, very few in vivo studies on IFPDMSCs for cartilage healing in OA have been reported. Aims: This systematic review will analyze the therapeutic potential of IFPDMSCs for cartilage healing in osteoarthritis from pre�clinical and clinical studies. Design, Methods, and Data Source: Using the PubMed, EMBASE, and Cochrane Library database up to November 30, 2020, a systematic review according to PRISMA reporting guideline was conducted on IFPDMSCs application to treat osteoarthritis in vivo studies. Inclusion criteria were in vivo preclinical and clinical studies from January 2010 to November 2020 involving the OA model or cases using IFPDMSCs to promote healing. Results: In vivo studies are scarce. Only four studies are included: two animals and two clinical studies. All included studies demonstrate favourable results of IFPDMSCs in osteoarthritis, but there is heterogeneity in outcome measure�ment among all studies. Conclusion: The in vitro and currently limited in vivo studies showed that infrapatellar fat pad-derived mesenchymal stem cells offer an alternative cell source with promising chondrogenic healing potential. Impact: More preclinical and clinical in vivo studies should be encouraged to explore and support th