The sustainable approach to the green space layout in highdensity urban environment: a case study of Macau peninsula

AbstractBased on a case study of Macau peninsula, this paper explores a sound approach to the urban green space development in high-density urban environment that could enhance the sustainability of the city

Bending behaviour of corroded RC continuous beams with C-FRCM strengthening system

This paper presents a numerical investigation into the bending behaviour of uncorroded and corroded reinforced concrete (RC) continuous beams with a strengthening system. Finite element analysis (FEA) was performed on ten RC beams considering interface performance, including five uncorroded and five corroded beams. The cracks development, bending capacities and load-displacement curves of the simulated RC beams in the loading process were validated against those from tests. Then, a parametric study including 35 RC beam models, considering the effects of carbon-fabric (CF) mesh layer, complete wrapping layer and the degree of corrosion of steel bar on their bending capacities, was conducted. Ductility and strengthening effects of specimens were discussed in the parametric study. It can be found that the carbon-fabric reinforced cementitious matrix (C-FRCM) strengthening system can improve the bending capacities of the corroded RC beams. As the layer of CF mesh increases, the ductility of the specimen decreases. The combined use of the C-FRCM plate and the complete wrapping as the end anchorage enhanced the ultimate loads of RC beams to a greater extent than those strengthened with C-FRCM plate only. The applicability of current design codes for RC beams with C-FRCM strengthening system was examined through comparisons of the bending capacity predictions of RC beams with those obtained from tests and numerical analyses. It was found that European Code (FIB Bulletin 14) provides more accurate predictions than American Specifications (ACI 549.4R-20, AC 434-0616-R1, ACI 440.2R-17) and Chinese Code (CECS 146–2003 (2007)). Therefore, design modifications based on the most accurate design rule of FIB Bulletin 14 were made. By utilizing regression analysis on the numerical results, the formula for bending capacities of the examined RC beams was proposed and showed improved accuracy

Speculation on optimal numbers of examined lymph node for early-stage epithelial ovarian cancer from the perspective of stage migration

IntroductionIn early-stage epithelial ovarian cancer (EOC), how to perform lymphadenectomy to avoid stage migration and achieve reliable targeted excision has not been explored in depth. This study comprehensively considered the stage migration and survival to determine appropriate numbers of examined lymph node (ELN) for early-stage EOC and high-grade serous ovarian cancer (HGSOC).MethodsFrom the Surveillance, Epidemiology, and End Results database, we obtained 10372 EOC cases with stage T1M0 and ELN ≥ 2, including 2849 HGSOC cases. Generalized linear models with multivariable adjustment were used to analyze associations between ELN numbers and lymph node stage migration, survival and positive lymph node (PLN). LOESS regression characterized dynamic trends of above associations followed by Chow test to determine structural breakpoints of ELN numbers. Survival curves were plotted using Kaplan-Meier method.ResultsMore ELNs were associated with more node-positive diseases, more PLNs and better prognosis. ELN structural breakpoints were different in subgroups of early-stage EOC, which for node stage migration or PLN were more than those for improving outcomes. The meaning of ELN structural breakpoint varied with its location and the morphology of LOESS curve. To avoid stage migration, the optimal ELN for early-stage EOC was 29 and the minimal ELN for HGSOC was 24. For better survival, appropriate ELN number were 13 and 8 respectively. More ELNs explained better prognosis only at a certain range.DiscussionNeither too many nor too few numbers of ELN were ideal for early-stage EOC and HGSOC. Excision with appropriate numbers of lymph node draining the affected ovary may be more reasonable than traditional sentinel lymph node resection and systematic lymphadenectomy

IGFBP-rP1, a potential molecule associated with colon cancer differentiation

<p>Abstract</p> <p>Background</p> <p>In our previous studies, we have demonstrated that insulin-like growth factor binding protein-related protein1 (IGFBP-rP1) played its potential tumor suppressor role in colon cancer cells through apoptosis and senescence induction. In this study, we will further uncover the role of IGFBP-rP1 in colon cancer differentiation and a possible mechanism by revealing responsible genes.</p> <p>Results</p> <p>In normal colon epithelium, immunohistochemistry staining detected a gradient IGFBP-rP1 expression along the axis of the crypt. IGFBP-rP1 strongly expressed in the differentiated cells at the surface of the colon epithelium, while weakly expressed at the crypt base. In colon cancer tissues, the expression of IGFBP-rP1 correlated positively with the differentiation status. IGFBP-rP1 strongly expressed in low grade colorectal carcinoma and weakly expressed in high grade colorectal carcinoma. In vitro, transfection of PcDNA3.1(IGFBP-rP1) into RKO, SW620 and CW2 cells induced a more pronounced anterior-posterior polarity morphology, accompanied by upregulation with alkaline phosphatase (AKP) activity. Upregulation of carcino-embryonic antigen (CEA) was also observed in SW620 and CW2 transfectants. The addition of IGFBP-rP1 protein into the medium could mimic most but not all effects of IGFBP-rP1 cDNA transfection. Seventy-eight reproducibly differentially expressed genes were detected in PcDNA3.1(IGFBP-rP1)-RKO transfectants, using Affymetrix 133 plus 2.0 expression chip platform. Directed Acyclic Graph (DAG) of the enriched GO categories demonstrated that differential expression of the enzyme regulator activity genes together with cytoskeleton and actin binding genes were significant. IGFBP-rP1 could upreguate Transgelin (TAGLN), downregulate SRY (sex determining region Y)-box 9(campomelic dysplasia, autosomal sex-reversal) (SOX9), insulin receptor substrate 1(IRS1), cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), amphiregulin(schwannoma-derived growth factor) (AREG) and immediate early response 5-like(IER5L) in RKO, SW620 and CW2 colon cancer cells, verified by Real time Reverse Transcription Polymerase Chain Reaction (rtRT-PCR). During sodium butyrate-induced Caco2 cell differentiation, IGFBP-rP1 was upregulated and the expression showed significant correlation with the AKP activity. The downregulation of IRS1 and SOX9 were also induced by sodium butyrate.</p> <p>Conclusion</p> <p>IGFBP-rP1 was a potential key molecule associated with colon cancer differentiation. Downregulation of IRS1 and SOX9 may the possible key downstream genes involved in the process.</p

FTO gene polymorphisms and obesity risk: a meta-analysis

<p>Abstract</p> <p>Background</p> <p>The pathogenesis of obesity is reportedly related to variations in the fat mass and an obesity-associated gene (<it>FTO</it>); however, as the number of reports increases, particularly with respect to varying ethnicities, there is a need to determine more precisely the effect sizes in each ethnic group. In addition, some reports have claimed ethnic-specific associations with alternative SNPs, and to that end there has been a degree of confusion.</p> <p>Methods</p> <p>We searched PubMed, MEDLINE, Web of Science, EMBASE, and BIOSIS Preview to identify studies investigating the associations between the five polymorphisms and obesity risk. Individual study odds ratios (OR) and their 95% confidence intervals (CI) were estimated using per-allele comparison. Summary ORs were estimated using a random effects model.</p> <p>Results</p> <p>We identified 59 eligible case-control studies in 27 articles, investigating 41,734 obesity cases and 69,837 healthy controls. Significant associations were detected between obesity risk and the five polymorphisms: rs9939609 (OR: 1.31, 95% CI: 1.26 to 1.36), rs1421085 (OR: 1.43, 95% CI: 1.33 to 1.53), rs8050136 (OR: 1.25, 95% CI: 1.13 to 1.38), rs17817449 (OR: 1.54, 95% CI: 1.41 to 1.68), and rs1121980 (OR: 1.34, 95% CI: 1.10 to 1.62). Begg's and Egger's tests provided no evidence of publication bias for the polymorphisms except rs1121980. There is evidence of higher heterogeneity, with <it>I</it>²test values ranging from 38.1% to 84.5%.</p> <p>Conclusions</p> <p>This meta-analysis suggests that <it>FTO </it>may represent a low-penetrance susceptible gene for obesity risk. Individual studies with large sample size are needed to further evaluate the associations between the polymorphisms and obesity risk in various ethnic populations.</p

Evaluation of the Development of Circular Agriculture in Chongqing City Based on Entropy Method

The circular agriculture is conducive to achieving the purpose of efficient agricultural economy and environment-friendliness. Based on the perspective of circular agriculture theory, this paper builds the evaluation indicator system for circular agriculture, and uses entropy method and Matlab analysis tool to carry out the comprehensive evaluation of the level of agricultural development in Chongqing City during the period 2003-2012. It analyzes the obstacles to the development of circular agricultural economy in Chongqing City in 2012, and finally puts forward the corresponding recommendations

Experimental and analytical study of hollow section concrete-filled GFRP tubes in bending

The flexural response of hollow section concrete-filled GFRP tubular (HS-CFGT) members has been examined experimentally and reported in this paper. A total of 14 specimens was tested, including four-point bending tests on reduced-scale specimens in the laboratory and cantilever bending tests on full-scale specimens on the construction site. The details of the test rigs and procedures, as well as the key test observations, including the ultimate moment capacities, load–displacement curves and failure modes, are fully reported. The test results were analysed to evaluate the influence of key variables on the structural performance of the HS-CFGT tubes, including concrete infill, GFRP tubes, slips between concrete and GFRP tubes and confinement of concrete infill. Fibre analysis approach was adopted to determine the moment capacity of the examined HS-CFGT sections. A total of four representative stress–strain curves of concrete were evaluated for their applicability to the HS-CFGT sections in bending. It was found that the concrete models proposed for general FRP-confined concrete columns are not applicable to the examined HS-CFGT sections in bending, and the uniaxial concrete model leads to rather conservative moment predictions, whereas, the FRP-confined concrete model used for beams provides great accuracy in predicting the moment capacities for both reduced-scale and full-scale HS-CFGT members