File S1-4 and Table S1

File S1-4 and Table S

Measurement results for different kinds of subjects.

<p>(A) Thirty seconds video of a one-month old infant was recorded at 30fps when he was sleeping. The face area and shoulder area are selected as the ROI for cardiovascular pulse measurement and respiration measurement, respectively (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g007" target="_blank">Figure 7A</a>). In the spectrum of the BVP signal (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g007" target="_blank">Figure 7H</a>), a clear peak at 2.15 Hz corresponds to the HR of 129 bpm. In the spectrum of the breath wave signal (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g007" target="_blank">Figure 7I</a>), an obvious peak at 0.73 Hz corresponds to the RR of 44 breaths/min. Written consent from the child’s parents had been obtained. (B) A high speed camera (Stingray F-033B/C with 80fps) was used to capture images of a mouse at rest over a period of about 10 seconds. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g008" target="_blank">Figure 8</a> demonstrates the procedure of recovering the HR and RR of a mouse from a single channel images. The measurement result of RR is 230 breaths/min and the measurement result of HR is 686 bpm. (C) For measuring adult zebrafish (roy/roy; alb/alb), we used tricaine to slightly anesthetize before the images were captured at 15 fps over a period of about 30 seconds in length. The ROI for cardiovascular pulse measurement and respiration measurement is the heart area and gilles area, respectively (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g009" target="_blank">Figure 9A</a>). The measurement result for HR is 78 bpm and for RR is 102 breaths/min (for signal plot, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g009" target="_blank">Figure 9</a>). (D) The face area and abdomen area of the pig are chosen as the ROI for cardiovascular pulse measurement and respiration measurement, respectively (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g010" target="_blank">Figure 10A</a>). The measurement results of HR and RR are 64 bpm and 19 breaths/min, respectively (for signal plot, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g010" target="_blank">Figure 10</a>). (E) A video of Phelps before 200 M butterfly competition downloaded from YouTube (<a href="http://www.youtube.com/watch?v=ftHlLqamWlM" target="_blank">http://www.youtube.com/watch?v=ftHlLqamWlM</a>). The recovered HR from 3 seconds time period selected from the video is 49 bpm. (F) Post-race interview of Michael Phelps (after his 200 Fly meet) downloaded from YouTube (<a href="http://www.youtube.com/watch?v=HHy7QKEV310" target="_blank">http://www.youtube.com/watch?v=HHy7QKEV310</a>). The interview video was recorded at 29 fps with pixel resolution of 1280×720. Two time periods both about 3 seconds in length were selected from the video to recover the HR of Phelps (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g011" target="_blank">Figure 11</a>). The time interval between two time periods is within one minute. The measurement results of HR for the first and second time periods are 123bpm and 126bpm, respectively. The RR was also extracted from the video over a period of about 7 seconds. The measurement result of RR is 28 breaths/min (for signal plot, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone-0071384-g012" target="_blank">Figure 12</a>). Reprinted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone.0071384-http1" target="_blank">[43]</a> under a CC BY license, with permission from [Longhorn Network], original copyright [2012]</p

Examples of false signals recognition.

<p>(A) Take a Simpsons cartoon character as an example. (B) The observed time series of the Simpsons cartoon is decomposed to extract (C) the source signal and (D) its power spectrum. (E) The smoothed source signal and (F) its power spectrum. (G) Another example is the Mona Lisa painting. (H) The observed time series of the Mona Lisa painting is decomposed to extract (I) the source signal and (J) its power spectrum. (K) The smoothed source signal and (L) its power spectrum.</p

Measurement of a pig.

<p>(A) The rose and hyacinthine rectangle superimposed on the pig indicate the ROI for cardiovascular pulse measurement and respiratory measurement, respectively. 30 seconds observed time series for cardiovascular pulse measurement (B) and respiratory measurement (C). The separated source signals for cardiovascular pulse measurement (D) and respiratory measurement (E). The recovered blood volume pulse (F) and respiratory wave (G). The spectrums of the blood volume pulse (H) and respiratory wave (I).</p

Measurement of a zebrafish.

<p>(A) The rose and hyacinthine rectangle superimposed on the zebrafish indicate the ROI for cardiovascular pulse measurement and respiratory measurement, respectively. 30 seconds observed time series for cardiovascular pulse measurement (B) and respiratory measurement (C). The separated source signals for cardiovascular pulse measurement (D) and respiratory measurement (E). The recovered blood volume pulse (F) and respiratory wave (G). The spectrums of the blood volume pulse (H) and respiratory wave (I).</p

Extracting HR from Michael Phelps video obtained from YouTube.

<p>All the red curves correspond to the first time period, and all the purple curves correspond to the second time period. The rectangles superimposed on his chest indicate the ROI for the first time period (A) and the second time period (B). 3 seconds observed time series from the first time period (C) and the second time period (D). The separated source signals for the first time period (E) and the second time period (F). The recovered blood volume pulses for the first time period (G) and the second time period (H). The spectrum of the blood volume pulses for the first time period (I) and the second time period (J). Reprinted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone.0071384-http1" target="_blank">[43]</a> under a CC BY license, with permission from [Longhorn Network], original copyright [2012].</p

Dynamic measurement of both HR and RR.

<p>(A) A plot of HR measured in a subject as a function of time. (B) A plot of RR measured in a subject as a function of time. In both cases, physiological parameters were measured in a subject following moderate exercise.</p

<i>k</i> and <i>c</i>_<i>v</i> of RSC layers 5&6 principal cells were robust across different brain states.

<p><b>(A</b>) <i>k</i> of RSC layers 5&6 principal cells showed linear relation under the quiet awake and sleep states for. <b>(B)</b> <i>c</i>_<i>v</i> of RSC layers 5&6 principal cells showed linear relation under the quiet awake and sleep states.</p

Measurement of a mouse.

<p>(A) The triangle and rectangle superimposed on the mouse indicate the ROI for cardiovascular pulse measurement and respiratory measurement, respectively. 10 seconds observed time series for cardiovascular pulse measurement (B) and respiratory measurement (C). The separated source signals for cardiovascular pulse measurement (D) and respiratory measurement (E). The recovered blood volume pulse (F) and respiratory wave (G). The spectrums of the blood volume pulse (H) and respiratory wave (I).</p

Extracting RR from Michael Phelps post-race interview video obtained from YouTube (http://www.youtube.com/watch?v=HHy7QKEV310).

<p>(A) the rectangle superimposed on his chest indicates the ROI. (B) 7 seconds observed time series for respiratory measurement. (C) the separated source signals for respiratory measurement. (D) the recovered breath wave. (E) the frequency of the breath wave. Reprinted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071384#pone.0071384-http1" target="_blank">[43]</a> under a CC BY license, with permission from [Longhorn Network], original copyright [2012].</p