Additional file 8: Figure S2. of miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1

Gene networks visualized using Cytoscape3.3 and generated from both up- (red) and down- (blue) regulated gene sets in miR-27 KD neurons. (TIF 1178 kb

Additional file 5: Figure S1. of miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1

Microarray analysis of the presynaptic transcriptome in miR-27 KD (n = 3) and CT (n = 3) neurons. Shown are differentially-expressed genes (FDR < 0.05). (TIF 235 kb

La Banca dati vibrazioni quale strumento di semplificazione per la valutazione del rischio nel settore agricolo

List of down-regulated gene sets in miR-27 KD neurons. (XLSX 21 kb

Additional file 9: Figure S3. of miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1

Bassoon immunostaining in miR-27 KD and CT hippocampal neurons. (TIF 765 kb

Spectral analysis of EEG from channel Fz for both dexmedetomidine sedation and propofol sedation.

<p>(A-B) Mean power spectra of WA (red), MS (green), DS (blue) and RS (black) for the two agents. (C-D) The green line represents the bootstrapped mean spectra of the difference between MS and WA for the two agents, and the gray space represents the bootstrapped 95% confidence interval bounds for the difference. (E-F) The blue line represents the bootstrapped mean spectra of the difference between DS and WA for the two agents, and the gray space represents the bootstrapped 95% confidence interval bounds for the difference. (G-H) The black line represents the bootstrapped mean spectra of the difference between RS and WA for the two agents, and the gray space represents the bootstrapped 95% confidence interval bounds for the difference. (I-J) The blue line represents the bootstrapped mean spectra of the difference between DS and MS for the two agents, and the gray space represents the bootstrapped 95% confidence interval bounds for the difference. The horizontal solid red lines represent the frequency ranges at which significant differences exist between each sedation state and WA, and the solid green lines represent the frequency ranges at which significant differences exist between DS and MS. WA, wakefulness; MS, moderate sedation; DS, deep sedation; RS, recovery state (RS).</p

Additional file 4: Table S4. of miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1

Comparative analysis of the presynaptic transcriptome in miR-27b KD neurons by nCounter or microarray profiling. (XLSX 11 kb

Experimental paradigm.

<p>Volunteers underwent Bispectral monitoring and EEG recording during each of the four experimental sessions: wakefulness, moderate sedation (OAA/S = 3), deep sedation (OAA/S = 1), and recovery (OAA/S = 5). Dexmedetomidine or propofol was administered intravenously and titrated to achieve the required sedation level.</p