Protein Engineering Potential Inhibitor of Detrimental Immune Responses

On the surface of immune cells, class II major histocompatibility complex proteins (MHCII) present antigenic peptides for CD4+ T cell recognition, which initiate a variety of antigen-specific immune responses such as antibody response or cytotoxic T cell activation. In people with with auto-immune diseases including but not limited to type 1 diabetes, multiple sclerosis, and rheumatoid arthritis, detrimental immune responses occur after the presentation of antigenic peptides. A single-chain, minimal MHCII (scm-MHCII) has been designed to retain its function as an antigen-presenting protein with a simplified structure that can be easily produced and manipulated in a laboratory by recombinant microbial expression. By applying directed evolution and selection for protein stability quantified using yeast surface display (YSD), we have engineered a mutant library which may contain highly stable mutants capable of functioning as a highly specific inhibitor of T cell-mediated immune responses with the potential to be applied to treating a variety auto-immune diseases

Helical edge and surface states in HgTe quantum wells and bulk insulators

The quantum spin Hall (QSH) effect is the property of a new state of matter which preserves time-reversal, has an energy gap in the bulk, but has topologically robust gapless states at the edge. Recently, it has been shown that HgTe quantum wells realize this novel effect. In this work, we start from realistic tight-binding models and demonstrate the existence of the helical edge states in HgTe quantum wells and calculate their physical properties. We also show that 3d HgTe is a topological insulator under uniaxial strain, and show that the surface states are described by single-component massless relativistic Dirac fermions in 2+1 dimensions. Experimental predictions are made based on the quantitative results obtained from realistic calculations.Comment: 5 page

Progressive Collapse Resistance of Precast Concrete Beam-Column Sub-assemblages with High-Performance Dry Connections

Due to its relatively lower integrity, precast concrete structures are considered to be more vulnerable to progressive collapse than cast-in-place concrete structures. However, to date, majority of existing studies on progressive collapse focused on cast-in-place concrete structures, little attentions were paid to precast concrete structures. Among existing precast concrete structures, unbonded post-tensioning precast concrete structure is one of innovation dry connection structural systems, which no casting at the connections on site. Its excellent seismic performance was recognized by many studies, while studies on its progressive collapse resistance were very few. To fill this knowledge gaps, in this paper, eight half-scaled unbonded post-tensioning precast concrete beam-column sub-assemblages with different connection configurations were tested through pushdown tests to investigate their capacities and resistance mechanisms to prevent progressive collapse. The test results demonstrated various behaviors of beam-column sub-assemblages with different connection types. It was found that, as the longitudinal reinforcements were discontinuous across the beam-column joint region in the beams, flexural action observed in the cast-in-place concrete frames was not mobilized for the specimens with purely unbonded post-tensioning connections. When the specimens installed top-seat angles at the beam-column interfaces, considerable flexural action capacity could be mobilized for load resistance. Moreover, it was found that the failure modes of the specimens are distinctly different to that of conventional reinforced concrete frames or precast concrete frames with cast-in-place joints. The characteristic of compressive arch action and tensile catenary action in tested specimens is quite different to that of conventional reinforced concrete frames

Quantum Anomalous Hall Effect in Hg1−y_{1-y}Mny_{y}Te Quantum Wells

The quantum Hall effect is usually observed when the two-dimensional electron gas is subjected to an external magnetic field, so that their quantum states form Landau levels. In this work we predict that a new phenomenon, the quantum anomalous Hall effect, can be realized in Hg$_{1-y}$Mn$_{y}$Te quantum wells, without the external magnetic field and the associated Landau levels. This effect arises purely from the spin polarization of the $Mn$ atoms, and the quantized Hall conductance is predicted for a range of quantum well thickness and the concentration of the $Mn$ atoms. This effect enables dissipationless charge current in spintronics devices.Comment: 5 pages, 3 figures. For high resolution figures see final published version when availabl

Band Narrowing and Mott Localization in Iron Oxychalcogenides La2O2Fe2O(Se,S)2

Bad metal properties have motivated a description of the parent iron pnictides as correlated metals on the verge of Mott localization. What has been unclear is whether interactions can push these and related compounds to the Mott insulating side of the phase diagram. Here we consider the iron oxychalcogenides La2O2Fe2O(Se,S)2, which contain an Fe square lattice with an expanded unit cell. We show theoretically that they contain enhanced correlation effects through band narrowing compared to LaOFeAs, and we provide experimental evidence that they are Mott insulators with moderate charge gaps. We also discuss the magnetic properties in terms of a Heisenberg model with frustrating J1-J2-J2' exchange interactions on a "doubled" checkerboard lattice.Comment: 4 pages, 5 eps figures. Version to appear in Phys. Rev. Let

Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation.

Extracellular vesicles (EVs) have been identified as cell-cell communication agents, and EVs derived from mesenchymal stem cells (MSCs) exhibit therapeutic effects similar to those of the cells of origin. Precipitation methods have been used extensively for EV harvests, such as UC- (ultracentrifugation-) or PEG- (polyethylene glycol-) based methods, and the difference in EVs derived from MSCs by UC and PEG is not fully understood. We harvested EVs from amniotic fluid MSCs (AF-MSCs) by UC- or PEG-based precipitation methods and conducted a comparison study of those EVs derived by the two methods: output, RNA, and protein expression of EVs and EV biological reaction in a THP-1-cell model of LPS induction, which was considered an infection model. There was no difference in morphology, size, or specific marker-positive ratio of PEG-EVs and UC-EVs, but PEG obtained more EV particles, protein, and RNA than the UC method. In our THP-1 model of LPS induction, MSC-EVs did not lead to a change in protein expression but inhibited the LPS-induced increase in cytokine secretion. UC-EVs were more effective for TNF-α inhibition, and PEG-EVs were more effective for IL10 inhibition. Thus, our findings provide evidence that PEG-based precipitation is a more efficient mesenchymal stem cell-extracellular vesicle-derived method than UC

Exclusive B→VVB \to VV Decays and CP Violation in the General two-Higgs-doublet Model

Using the general factorization approach, we present a detailed investigation for the branching ratios, CP asymmetries and longitudinal polarization fractions in all charmless hadronic $B \to VV$ decays (except for the pure annihilation processes) within the most general two-Higgs-doublet model with spontaneous CP violation. It is seen that such a new physics model only has very small contributions to the branching ratios and longitudinal polarization fractions. However, as the model has rich CP-violating sources, it can lead to significant effects on the CP asymmetries, especially on those of penguin-dominated decay modes, which provides good signals for probing new physics beyond the SM in the future B-physics experiments.Comment: 17 pages, no figure