15 research outputs found
8-Bromo-3-(cycloÂpropanylcarbonÂyl)-5-methylÂindolizine-1-carbonitrile
The asymmetric unit of the title compound, C14H11BrN2O, contains three independent molÂecules with very similar geometries. The dihedral angles between the side chain of the cycloÂpropyl plane and the five-membered ring to which it is attached are 55.0 (2), 58.1 (2) and 60.2 (3)° for the three molÂecules. Each molÂecule forms an intraÂmolecular C—H⋯O hydrogen bond
Porous Organic Cage Embedded C18 Amide Silica Stationary Phase for High Performance Liquid Chromatography
Hyperbranched Polyglycerol Functionalized Silica Stationary Phase for Hydrophilic Interaction Liquid Chromatography
Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive Enzyme-Linked Immunosorbent Assay
A sensitive biotinylated indirect competitive enzyme-linked immunosorbent assay (Bic-ELISA) was developed to detect acetamiprid pesticides in pollen, based on the heterogeneous coating antigen and biotinylated anti-acetamiprid monoclonal antibody. Under optimized experimental conditions, the detection limit for the Bic-ELISA was 0.17 ng/mL and the linear range was 0.25–25 ng/mL. The cross-reactivities could be regarded as negligible for the biotinylated antibodies with their analogues except for thiacloprid (1.66%). Analyte recoveries for extracts of spiked pollen (camellia pollen, lotus pollen, rape pollen) ranged from 81.1% to 108.0%, with intra-day relative standard deviations (RSDs) of 4.8% to 10.9%, and the average reproducibility was 85.4% to 110.9% with inter-assay and inter-assay RSDs of 6.1% to 11.7%. The results of Bic-ELISA methods for the Taobao’s website samples were largely consistent with HPLC-MS/MS. Therefore, the established Bic-ELISA methods would be conducive to the monitoring of acetamiprid in pollen