EFEMP1 binds the EGF receptor and activates MAPK and Akt pathways in pancreatic carcinoma cells

The EGF-related protein EFEMP1 (EGF-containing fibulin-like extracellular matrix protein 1) has been shown to promote tumor growth in human adenocarcinoma. To understand the mechanism of this action, the signal transduction activated upon treatment with this protein has been investigated. We show that EFEMP1 binds EGF receptor (EGFR) in a competitive manner relative to epidermal growth factor (EGF), implicating that EFEMP1 and EGF share the same or adjacent binding sites on the EGFR. Treatment of pancreatic carcinoma cells with purified EFEMP1 activates autophosphorylation of EGFR at the positions Tyr-992 and Tyr-1068, but not at the position Tyr-1048. This signal is further transduced to phosphorylation of Akt at position Thr-308 and p44/p42 MAPK (mitogen-activated protein kinase) at positions Thr-202 and Tyr-204. These downstream phosphorylation events can be inhibited by treatment with the EGFR kinase inhibitor PD 153035. The observed signal transduction upon treatment with EFEMP1 can contribute to the enhancement of tumor growth shown in pancreatic carcinoma cells overexpressing EFEMP1

EFEMP1 binds the EGF receptor and activates MAPK and Akt pathways in pancreatic carcinoma cells

The EGF-related protein EFEMP1 (EGF-containing fibulin-like extracellular matrix protein 1) has been shown to promote tumor growth in human adenocarcinoma. To understand the mechanism of this action, the signal transduction activated upon treatment with this protein has been investigated. We show that EFEMP1 binds EGF receptor (EGFR) in a competitive manner relative to epidermal growth factor (EGF), implicating that EFEMP1 and EGF share the same or adjacent binding sites on the EGFR. Treatment of pancreatic carcinoma cells with purified EFEMP1 activates autophosphorylation of EGFR at the positions Tyr-992 and Tyr-1068, but not at the position Tyr-1048. This signal is further transduced to phosphorylation of Akt at position Thr-308 and p44/p42 MAPK (mitogen-activated protein kinase) at positions Thr-202 and Tyr-204. These downstream phosphorylation events can be inhibited by treatment with the EGFR kinase inhibitor PD 153035. The observed signal transduction upon treatment with EFEMP1 can contribute to the enhancement of tumor growth shown in pancreatic carcinoma cells overexpressing EFEMP1

Basic Principles and Utilization Possibilities’ of Ultrasonic Phased Array in Material Nondestructive Evaluation

The paper deals with the basic principles of operation and with the utilization possibilities of phased array (PA) in materials nondestructive testing (NDT). The first part deals with description of PA arrangement modes, which enable to generate, focus and steer the ultrasonic beem. The second part deals with the description of electromagnetic acoustic transducer PA operation. The last part deals with the description of the utilization of PA in nondestructive testing of conductive materials and the advantages of PA utilization in inhomogeneous materials NDT

Electromagnetic Phenomena as the Principles of Material Nondestructive Evalution

In the paper the principles and application of nondestructive evalution by both electromagnetic acoustic transducer (EMAT) and the eddy current (EC) methods are introducted. The ultrasonic testing (UT) and the electromagnetic nondestructive testing (NDT) are presented with their fundamental properties and the experimental results concerning the defect of conducting object characterization are shown

Metamaterial Structures and Possibility of their Application in Microwave Applicator Optimization

Metamaterials are special one-, two- or three-dimensional artificial structures with electromagnetic properties generally not found in nature. Due to the simultaneous negative values of permittivity (ε) and permeability (μ), the wave vector and the vectors of electric- and magnetic-field intensity form a left-handed triplet with the result of antiparallel phase and group velocity and backwave propagation. These unique properties of the left-handed materials have allowed novel applications and devices to be developed. In this paper the metamaterial structures based on the split ring resonator approach for optimization of microwave applicator characteristic are used. The main field of the study is absorption of microwave-frequency field in human arm model and improvement of microwave applicator properties such a directivity and gain by means of simulations performed in CST Microwave Studio environment

Metallic Structures Based on Zinc Oxide Film for Enzyme Biorecognition

Two structures (Ag/ZnO/ITI/glass: #1 sample and Ag/ZnO/SiO2/Si: #2 sample) are investigated, on the one hand, from the point of view of the formation of evanescent waves in the gratings of metal strips on the structures when the incident TEz wave in the radio frequency range is used. The simulation of the formation of evanescent waves at the edge of the Ag strips, with thicknesses in the range of micrometers, was carried out before the test in the subwavelength regime, with the help of a new improved transducer with metamaterial (MM) lenses. By simulation, a field snapshot was obtained in each sequence of geometry. The evanescent waves are emphasized in the plane XY, due to the scattering of the field on the edge of the strips. On the other hand, ZnO nanoparticles are investigated as a convenient high-efficiency biodetection material, where these structures were used as a biosensitive element to various enzymes (glucose, cholesterol, uric acid, and ascorbic acid). The obtained results demonstrate that the investigated structures based on ZnO nanostructures deposited on different supports are fast and sensitive for enzyme detection and can be successfully incorporated into a device as a biosensing element

Enhancement of spatial resolution using metamaterial sensor in NonDestructive evaluation

International audienceThis paper presents a possibility to enhance the spatial resolution of eNDE methods that operate in radiofrequency and high frequency range using a sensor with metamaterial lens that can manipulate the evanescent waves that appear in the space between strips and respective, carbon fibers. Also, interruptions, short-cuts of MSG as well as nonalignment of carbon fibers, lack of resin or voids, and delaminations induced by impacts with low energy can be detected and emphasized. © 2016 Taylor and Francis Group, London

Enhancement of spatial resolution using metamaterial sensor in NonDestructive evaluation

International audienceThis paper presents a possibility to enhance the spatial resolution of eNDE methods that operate in radiofrequency and high frequency range using a sensor with metamaterial lens that can manipulate the evanescent waves that appear in the space between strips and respective, carbon fibers. Also, interruptions, short-cuts of MSG as well as nonalignment of carbon fibers, lack of resin or voids, and delaminations induced by impacts with low energy can be detected and emphasized. © 2016 Taylor and Francis Group, London