Heterogeneous agglomeration

Many prior treatments of agglomeration either explicitly or implicitly suppose that all industries agglomerate for the same reasons, with traditional Marshallian (1890) factors affecting all industries similarly. An important instance of this approach is the extrapolation of the agglomeration experience of one key sector or cluster to the larger economy. Another is the pooling of data to look at common tendencies in agglomeration. This paper uses UK establishment level data on coagglomeration to document heterogeneity across industries in the microfoundations of agglomeration economies. The pattern of heterogeneity that we document is consistent with both traditional Marshallian theories and with alternative approaches that emphasize the adaptive and organizational aspects of agglomeration. *Disclaimer: This work was based on data from the Business Structure Database and the Quarterly UK Labour Force Survey, produced by the Office for National Statistics (ONS) and supplied by the Secure Data Service at the UK Data Archive. The data are Crown Copyright and reproduced with the permission of the controller of HMSO and Queen's Printer for Scotland. The use of the data in this work does not imply the endorsement of ONS or the Secure Data Service at the UK Data Archive in relation to the interpretation or analysis of the data. This work uses research datasets which may not exactly reproduce National Statistics aggregates

Effect of water quality on hematological and biochemical parameters of Gobius niger caught in Faro Lake (Sicily)

In many countries, environmental risk assessment is still based solely on chemical analyses that do not suffice to evaluate the complex toxic effects on the environment. This latter derives from the combined effects of chemicals, their decomposition products, and the physical environment can only be evaluated by biological test using biomarkers (McCarthy and Shugart, 1990). Biomarker analysis of field-collected organisms can provide information on the status of the environment, avoiding the need and uncertainty inherent to the extrapolation of laboratory results (Menezes et al., 2006). Biomarkers are defined as a change in a biological response, ranging from molecular to cellular and from physiological responses to behavioural changes which can be related to the toxic exposure or to the toxic effects of environmental chemicals (Depledge et al., 1995). The use of selected biomarkers has become attractive and useful for monitoring environmental quality and the health of fish inhabiting polluted ecosystems (Fernandes et al., 2008). Fish are largely used for the assessment of aquatic environment quality and are accepted as bio-indicators of environmental pollution (Borkovic et al., 2008). Fish, in fact, live in very intimate contact with their environment, and are therefore very susceptible to physical and chemical changes which may be reflected in their blood components. It should be noted that haematological indices are of different sensitivity to various environmental factors and chemicals (Adeyemo, 2007)

Serum proteins profile in Comisana lambs during the first month of life

Abstract. The aim of the present study was to measure the serum total proteins and the serum protein fractions (albumin, α1-, α2-, β-, and γ- globulins) of ten newborn lambs (Comisana breed) during the first 30 days of life in order to obtain useful information for neonatal care. From each animal, blood samples were collected via jugular venipuncture at the same hour (9.00) every 3 days for 30 days starting from birth (day 0). The concentrations of serum total proteins and albumin, α1-, α2-, β-, γ- globulins, and Albumin/Globulins (A/G) ratio were determined using an automated electrophoresis system. One-way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, was used to determine significant differences between mean values of the studied parameters from the 1st to the 30th day of the experimental period. Data analysis of variance showed a statistical effect of days of life on total proteins, albumin, α1-globulins, β-globulins, γ-globulins and ratio A/G during the first 30 days of life (P<0.0001), while no statistical significant effect of days of life was observed on α2-globulins during the experimental period (P=0.27). The obtained results indicated that passive transfer status, determined from serum immunoglobulin concentration 24 hours after birth, is a significant source of variation in preweaning growth performance in dairy lambs. These finding make a contribution to the knowledge of physiological adaptation in lambs during the first 30 days of life and give useful information for the diagnosis and treatment of neonatal diseases

Red yeast (Phaffia rhodozyma) and its effect on growth, antioxidant activity and color pigmentation of rainbow trout (Oncorhynchus mykiss)

Efficacy of orally used of red yeast (Phaffia rhodozyma) (1.6 mg astaxanthin/g product) at 15.5 g (P1), 37.3 g (P2) and 47 (P3) g per kg diet were studied on performance, carcass composition, antioxidant activity and fillet pigmentation in rainbow trout (Oncorhynchus mykiss) weighing 208–212 g for eight weeks at 11 °C. Synthetic astaxanthin (AX) (0.5 g/kg diet) and basal diet were used as controls. With an increase in P. rhodozyma concentration in diet, weight gain and specific growth rate exhibited an increase compared to basal diet (P 0.05). Water holding capacity and lipid loss in fillets of treatments were lower than fish fed basal diet (P < 0.05). By increasing P. rhodozyma concentration in fish diets, redness value exhibited a progressive increase, and the highest value was seen in P3 diet compared to other treatments (P < 0.05). These data show application of P. rhodozyma at 47 g/kg diet in trout could provide a better performance, antioxidant activities, and fillet pigmentation

Oral administration of Gum Arabic: effects on haematological parameters and oxidative stress markers in Mugil cephalus

The aim of this research was to investigate the effects of the oral administration of Gum Arabic on haematological and some oxidative stress markers in Mugil cephalus. For this purpose 60 mullet caught in Faro Lake (Italy), were acclimatized and fed with commercial diet for 20 days prior to the start of experiment, which lasted 15 days. After acclimatization, fish were randomly divided into two equal groups: control group was fed with commercial pellets. Haematological profile on whole blood and sera was assessed, reactive oxygen metabolites (d-ROMs), antioxidant barrier (OXY-ads) and thiol-antioxidant barrier (SHp) were immediately assessed by means of a UV spectrophotometer. Two-way repeated data analysis (ANOVA) showed significant changes only on Thrombocyte Count (TC). In the experimental group an increase in OXY-ads and SHp levels and a significant decrease of d-ROMs were observed after administration of GA

Diet and Plastic Ingestion in the Blackmouth Catshark Galeus melastomus, Rafinesque 1810, in Italian Waters

Simple Summary The blackmouth catshark has a habitat range that spans from the Norwegian seas to Senegal and throughout the Mediterranean Sea, and it is one of the most common sharks in Italian waters. The aim of this work is to investigate, through the analyses of the stomach contents of five populations from the Tyrrhenian and Ionian Seas, the diet of blackmouth catsharks. The analyses showed that the most frequent items were Osteichthyes of the family Myctophidae, except for one population, in which the most common items were Cephalopods and Crustacean of the Decapods order. Plastic debris was also found in all populations analysed and classified by colour and shape. This study ought to increase the knowledge of the feeding ecology of the blackmouth catshark, thus improving the meagre literature about Tyrrhenian and Ionian waters. Galeus melastomus is the most common Pentanchidae in the Mediterranean Sea. A scavenger and opportunistic feeder, and despite the wide distribution, little is known about its feeding habits in Italian waters. The main purpose of this study was to investigate the diet of the blackmouth catshark by analysing the stomach contents. The specimens analysed were obtained from five populations of the Tyrrhenian and of the Ionian Seas, collected from a depth between 40 and 700 m. A total of 259 stomachs were analysed. The stomach contents were grouped into macro-categories and identified to the lowest taxonomic level possible. Crustaceans such as Parapenaeus longirostris, the Cephalopods Heteroteuthis dispar and Onychoteuthis banksii, and Osteichthyes, mostly Myctophidae, were identified. Plastic debris was also found among the stomach contents and classified according to its colour and shape. Osteichthyes represent the most abundant item (44%), above all the Myctophidae family, except for the catshark population from Tuscany, in which the most frequent species were Cephalopods, such as Abralia veranyi and Heteroteuthis dispar. Differences in the plastic debris contents were also observed between the Tuscany population and other populations. These could be explained as a probable consequence of the different depths at which the blackmouth catshark populations were sampled

Characterization of speech and language phenotype in GLUT1DS

Background: To analyze the oral motor, speech and language phenotype in a sample of pediatric patients with GLUT 1 transporter deficiency syndrome (GLUT1DS). Methods: eight Italian-speaking children with GLUT1DS (aged 4.6–15.4 years) in stable treatment with ketogenic diet from a variable time underwent a specific and standardized speech and language assessment battery. Results: All patients showed deficits with different degrees of impairment in multiple speech and language areas. In particular, orofacial praxis, parallel and total movements were the most impaired in the oromotor domain; in the speech domain patients obtained a poor performance in the diadochokinesis rate and in the repetition of words that resulted as severely deficient in seven out of eight patients; in the language domain the most affected abilities were semantic/phonological fluency and receptive grammar. Conclusions: GLUT1DS is associated to different levels of speech and language impairment, which should guide diagnostic and therapeutic intervention. Larger population data are needed to identify more precisely a speech and language profile in GLUT1DS patients

Edelfosine induced suicidal death of human erythrocytes

Background/Aims: The anti-inflammatory, anti-autoimmune, antiparasitic, and anti-viral ether phospholipid edelfosine (1-O-octadecyl-2-O-methylglycero-3-phosphocholine) stimulates apoptosis of tumor cells and is thus considered for the treatment of malignancy. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phospholipid scrambling of the cell membrane with phosphatidylserine translocation to the erythrocyte surface. Triggers of eryptosis include Ca2+ entry with increase of cytosolic Ca2+ activity ([Ca2+]i) and oxidative stress. The present study explored, whether and how edelfosine induces eryptosis. Methods: Flow cytometry and photometry, respectively, were employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, hemolysis from hemoglobin release, [Ca2+]i from Fluo3-fluorescence, and abundance of reactive oxygen species (ROS) from 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence. Results: A 6 hours exposure of human erythrocytes to edelfosine (5 \u3bcM) significantly increased the percentage of annexin-V-binding cells, significantly decreased forward scatter, and significantly increased Fluo3-fluorescence, but did not significantly modify DCFDA fluorescence. The effect of edelfosine on annexin-V-binding was significantly blunted, but not abolished by removal of extracellular Ca2+. Conclusions: Edelfosine triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect in part due to stimulation of Ca2+ entry

Nocodazole Induced Suicidal Death of Human Erythrocytes

Background: The microtubule assembly inhibitor nocodazole has been shown to trigger caspase-independent mitotic death and caspase dependent apoptosis. Similar to apoptosis of nucleated cells, erythrocytes may undergo eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+ activity ([Ca2+]i), oxidative stress and ceramide. The present study explored, whether and how nocodazole induces eryptosis. Methods: Flow cytometry was employed to determine phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, the abundance of reactive oxygen species (ROS) from 2\u2032,7\u2032-dichlorodihydrofluorescein (DCF) diacetate dependent fluorescence as well as ceramide surface abundance utilizing specific antibodies. Tubulin abundance was quantified by TubulinTracker\u2122 Green reagent and visualized by confocal microscopy. Results: A 48 hours exposure of human erythrocytes to nocodazole ( 65 30 \u3bcg/ml) significantly increased the percentage of annexin-V-binding cells without significantly modifying average forward scatter. Nocodazole significantly increased Fluo3-fluorescence, significantly increased DCF fluorescence and significantly increased ceramide surface abundance. The effect of nocodazole on annexin-V-binding was significantly blunted, but not abolished by removal of extracellular Ca2+ and was not modified in the presence of Caspase 3 inhibitor zVAD (1 \u3bcM). Nocodazole treatment reduced the content of total tubulin. Conclusions: Nocodazole triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect in part due to stimulation of Ca2+ entry, oxidative stress and ceramide