IN VIVO AND IN VITRO EFFECTS OF ARTEMISININ GROUP OF DRUGS ON TRYPANOSOMOSIS IN MICE

This study was done to study the effect of artemisinin groups of drugs on mice experimentally infected with the protozoan Trypanosoma brucei and on Trypanosoma brucei invitro. Commercial artesunate and artemether were used with diminazene aceturate serving as control. It was discovered that artemether administration resulted into seven days of aparasitaemia of trypanosomosis in vivo and reduced motility of the trypanosomes in vitro. There was synergistic effect in the action of artesunate and diminazene aceturate. In vitro analysis gave a similar result in that the trypanosome were found to be sensitive to artemether  with an MIC of 0.6µl,  artesunate with berenil combination and the artemether treatment group 2.5µM. The implication of these results is discussed and advice is given on the potential adaptation of artesunate for treatment of trypanosomosis and planting of Artemisia annua tree in the country.Â

Prevalencija invazije vrstama roda Cryptosporidium u goveda na području Jugozapadne Nigerije

This study investigates the prevalence of Cryptosporidium sp. infection in Oyo state, south western Nigeria. Fecal specimens from 406 cattle were randomly collected and examined for the presence of Cryptosporidium sp. oocysts using the formol ethyl ether concentration and the modified Kinyoun acid-fast staining method. The results showed that the overall prevalence of infection was 23.4% (95/406), with an infection rate of 27.4%, 28.1% and 19.9% in cattle less than 6 months, 7-12 months and over 12 months respectively. Although no significant difference (P>0.05) exists between the age groups, calves less than 6 months of age are more likely to be infected than adults (OR: 1.512; 95%CI: 0.849-2.709). A significant difference (P0,05) među skupinama različite dobi, smatra se da je telad mlađa od šest mjeseci primljivija na invaziju od odraslih. Značajna je razlika (P<0,0001) dokazana između prevalencije u goveda različitoga spola. U krava je dokazana dvostruko veća mogućnost invazije. Stopa invadiranosti iznosila je 84,2% (80/95) u goveda bez proljeva, a 15,8% (15/95) u goveda s proljevom. Nije ustanovljena povezanost između pojave proljeva i prisutnosti oocista kriptosporidija (P = 0,9468; OR: 0,979; 95%CI: 0,522-1,636). Rezultati pokazuju da je invazija protozoima Cryptosporidium sp. česta u Nigeriji, osobito u goveda koja ne pokazuju kliničke znakove bolesti, a mogu biti znatan izvor invazije za ljude

COMPARISON OF ENZYME-LINKED IMMUNOSORBENT ASSAY WITH ACID-FAST STAINING TO DETECT CRYPTOSPORIDIUM OF CATTLE FAECES

Cryptosporidiosis is a major cause of diarrhoea, weight loss and low productivity in various domestic animals. The acid-fast staining method used to detect the parasite in cattle may be responsible for the low prevalence rates recorded in previous studies in Nigeria. A comparison of the efficacy of an enzyme-linked immunosorbent assay (ELISA) and acid-fast staining techniques in detecting Cryptosporidium spp. in bovine faeces was carried out in this work. A total of 200 faecal samples were collected from cattle of different age groups by the use of a simple random sampling technique. The samples were analyzed microscopically using formalin-ethylacetate sedimentation method followed by modified Kinyoun’s acid-fast staining technique. All the samples were later tested for Cryptosporidium coproantigens by the use of a commercially available ELISA test kit. Microscopy detected Cryptosporidium oocysts in 24.0% while ELISA detected the antigens in 37.5% of the samples. The ELISA, with a sensitivity and specificity of 72.9% and 73.7% respectively, had a significantly higher (p&lt;0.05) rate of detection of Cryptosporidium spp. than microscopy with a sensitivity and specificity of 46.7% and 89.6% respectively. The ELISA is therefore a preferable method than microscopy for detection of Cryptosporidium in faecal specimens and will be useful in routine diagnosis and screening of large number of samples in epidemiological survey

COMPARISON OF ENZYME-LINKED IMMUNOSOR- BENT ASSAY WITH ACID-FAST STAINING TO DETECT CRYPTOSPORIDIUM OF CATTLE FAECES

Cryptosporidiosis is a major cause of diarrhoea, weight loss and low productivity in various domestic animals. The acid-fast staining method used to detect the parasite in cattle may be responsible for the low prevalence rates recorded in previous studies in Nigeria. A comparison of the efficacy of an en-zyme-linked immunosorbent assay (ELISA) and acid-fast staining techniques in detecting Crypto-sporidium spp. in bovine faeces was carried out in this work. A total of 200 faecal samples were col-lected from cattle of different age groups by the use of a simple random sampling technique. The sam-ples were analyzed microscopically using formalin-ethylacetate sedimentation method followed by modified Kinyoun«¤??s acid-fast staining technique. All the samples were later tested for Cryptosporidium coproantigens by the use of a commercially available ELISA test kit. Microscopy detected Crypto-sporidium oocysts in 24.0% while ELISA detected the antigens in 37.5% of the samples. The ELISA, with a sensitivity and specificity of 72.9% and 73.7% respectively, had a significantly higher (p&lt;0.05) rate of detection of Cryptosporidium spp. than microscopy with a sensitivity and specificity of 46.7% and 89.6% respectively. The ELISA is therefore a preferable method than microscopy for detection of Cryptosporidium in faecal specimens and will be useful in routine diagnosis and screening of large number of samples in epidemiological surveys

Transactivation of EGFR by LPS induces COX-2 expression in enterocytes

Necrotizing enterocolitis (NEC) is the leading cause of gastrointestinal morbidity and mortality in preterm infants. NEC is characterized by an exaggerated inflammatory response to bacterial flora leading to bowel necrosis. Bacterial lipopolysaccharide (LPS) mediates inflammation through TLR4 activation and is a key molecule in the pathogenesis of NEC. However, LPS also induces cyclooxygenase-2 (COX-2), which promotes intestinal barrier restitution through stimulation of intestinal cell survival, proliferation, and migration. Epidermal growth factor receptor (EGFR) activation prevents experimental NEC and may play a critical role in LPS-stimulated COX-2 production. We hypothesized that EGFR is required for LPS induction of COX-2 expression. Our data show that inhibiting EGFR kinase activity blocks LPS-induced COX-2 expression in small intestinal epithelial cells. LPS induction of COX-2 requires Src-family kinase signaling while LPS transactivation of EGFR requires matrix metalloprotease (MMP) activity. EGFR tyrosine kinase inhibitors block LPS stimulation of mitogen-activated protein kinase ERK, suggesting an important role of the MAPK/ERK pathway in EGFR-mediated COX-2 expression. LPS stimulates proliferation of IEC-6 cells, but this stimulation is inhibited with either the EGFR kinase inhibitor AG1478, or the selective COX-2 inhibitor Celecoxib. Taken together, these data show that EGFR plays an important role in LPS-induction of COX-2 expression in enterocytes, which may be one mechanism for EGF in inhibition of NEC

HLA-DQA1*05 carriage associated with development of anti-drug antibodies to infliximab and adalimumab in patients with Crohn's Disease

Anti-tumor necrosis factor (anti-TNF) therapies are the most widely used biologic drugs for treating immune-mediated diseases, but repeated administration can induce the formation of anti-drug antibodies. The ability to identify patients at increased risk for development of anti-drug antibodies would facilitate selection of therapy and use of preventative strategies.This article is freely available via Open Access. Click on Publisher URL to access the full-text

Short communication - A PRELIMINARY INVESTIGATION OF RESISTANCE TO ANTHELMINTICS IN STRONGYLES OF CATTLE IN SHAKI, NIGERIA

A survey was conducted and the occurrence of anthelmintic resistance in strongyles of cattle was detected. Ten herds of cattle in Shaki, Oyo state of Nigeria were studied. Larval Development Assay (LDA) was used as the test method. Four drugs were tested. Resistance to the four drugs were detected. Resistance to albendazole were detected in two herds and in one herd for febantel with LD50 > 0.10 μgml -1 . Resistance to levalmisole and morantel was detected in three and in two herds respectively with LD50 >1.0μgml -

Short communications - THE ASSESSMENT OF FASCIOLA GIGANTICA INFECTION IN THE RABBIT (ORYCTOLAGUS CUNICULUS) AS A LABORATORY MODEL PARASITE DEVELOPMENT - CLINICAL SYMPTOMS AND LIVER PATHOLOGY

In this study, the rabbit was assessed as a laboratory host for the helminthes parasite, Fasciola gigantica. Three groups of rabbits were Infected experimentally with 5, 10 end 15 metacercariae of F. gigantica respectively. Clinical signs found included pale mucous membrane, progressive emaciation and rough hair coat. The post mortem findings Included haemorrhage and scattered calcified nodules on the omentum. Echymosis. fibrosis and necrosis were prominent on (he Ever in addition to fibrosis and thickening of the gall bladder and bife ducts. Histopathology of the Ever revealed congestion. haemorrhage, ceffular Infiltration end necrosis, BE. ducts were hyper plastic and fibrotic with sever Inflammatory reactions. The ova of gigantica were recovered from the faeces of some infected rabbits at 10 weeks post infection. Mean number of flukes recovered from the rabbits was 0 in the 5 metacercariae group, 2 in the 10 metacercariae group and 5 in the group given 15 metacercariae