Phytochemical analysis, cytotoxicity and genotoxicity of Psidium guajava L. (Myrtaceae) leaf extracts

Psidium guajava is one of the oldest and widely used medicinal plants in the world. The leaves, bark, roots and immature fruits are used in African ethno-medicine because of their anti-inflammatory, anti-diarrhoeal and anti-microbial properties amongst others. The aim of this work was to determine the phytochemical profile of locally sourced mature Psidium guajava leaves and to also evaluate the cytotoxic and genotoxic properties of aqueous extracts of Psidium guajava leaves. Ethanol extracts of Psidium guajava leaves were subjected to analysis using GC-MS. Aqueous extracts were prepared by boiling pre-weighted fresh leaves in tap water and subsequently used in the Allium cepa assay. Analysis revealed terpene compounds (80.6%) as the most abundant phytochemical in ethanol extracts of the leaves. The results also revealed an overall significant concentration and time-dependent reduction in mitotic index (p ≤ 0.05). The value of EC50 was estimated to be 54.4 g/l. Examination of treated Allium cepa root tip cells revealed chromosomal aberrations including binucleation, bridges, distorted anaphase, clumping, vagrant chromosomes and c-mitosis. Aqueous leaf extracts of Psidium guajava leaves have genotoxic and cytotoxic potentials which should be seriously considered in its therapeutic applications

ASSESSMENT OF NUTRITIONAL PROPERTIES OF FERMENTED AND UNFERMENTED SEED OF CISSUS POPULNAE FROM NIGER STATE, NIGERIA

The assessment of nutritional properties of fermented (for 24 and 48 h) and unfermented seeds of Cissus populnae from Niger State, Nigeria were determined using standard analytical methods. The proximate parameters determined were moisture, protein, ash, fat, fiber and carbohydrate.  The ranges of these values were from 12.44±0.03 (unfermented) to 15.21±0.51 (fermented for 48 h), 4.23±0.16 (unfermented) to 5.04±0.15% (fermentation at 48 h), 2.00±0.05 (unfermented) to 3.10±0.45 (fermented for 48 h), 7.20±0.60 (unfermented) to 9.01±0.11), 4.02±0.23% (fermentation for 48 h) to 7.00±0.06 (unfermented) and 63.62±0.46 (fermented at 48 h) to 67.13±0.53 (unfermented) % for the moisture, crude fiber, ash, crude protein, crude fat and crude carbohydrate contents respectively. The energy values obtained were 1522.61±0.21, 1446.82±0.33 and 1383.45±0.50 Kcal/100g for the unfermented, fermentation for 24 and 48 h respectively. From the results of this study, fermentation generally improved the mineral contents of the samples and decreased their anti-nutrient contents. Thus, large-scale production of fermented Cissus populnae seeds will be a valuable source of nutrition to man and his animals

A longitudinal cohort study of symptoms and other concerns among Nigerian people with stages 3–5 chronic kidney diseases: study protocol

Background: The burden of symptoms and other concerns in chronic kidney disease (CKD) is known to be high, adversely affecting the quality of life of the growing number of those with this condition in developing countries. In this paper, we describe the protocol of a longitudinal observational study among people living with CKD. The study is developed to assess the bio-psychosocial factors associated with palliative care symptoms and concerns, and pattern of health services usage among Nigerians with stages 3–5 CKD. The overall objective is to establish the evidence-base for advocacy and policy formulation, treatment guidelines, care and services, and future clinical trial studies.Methods: This is a multi-center study to investigate the longitudinal course of symptoms and other concerns among patients with stages 3–5 CKD in Nigeria. Interviewer administered and self-report measures at baseline (T0) and 3-month (T1) address socio-demographic characteristics, clinical-illness related information, palliative care-related symptoms and other concerns, pattern of formal or informal service usage, and bio psychosocial measures including estimated glomerular filtration rate (eGFR), anxiety, depression, quality of life, functioning, social support and spiritual wellbeing.Discussion: This study represents the first longitudinal investigation of palliative care symptoms and concerns among people with CKD in Nigeria. It includes early stages of CKD in compliance with best practices, and a comprehensive range of bio-psychosocial outcomes to understand how these factors are associated with symptoms. This study will provide evidence for how best to integrate palliative care into management of CKD to improve care and quality of life of people with CKD. The study team welcomes collaborations with both national and international researchers

P A NEW CLASSES OF ANALYTIC FUNCTIONS

Abstract: Inequalities conditions for certain new classes of univalent functions were determined. Also,the distortion inequalities for the the new class of univalent functions was established

Workplace violence and risk for psychiatric morbidity among health workers in a tertiary health care setting in Nigeria: prevalence and correlates

The objectives were to evaluate the workplace violence and risk for psychiatric morbidity, as well as their correlates, among health workers in a tertiary healthcare setting in Nigeria. A stratified random sampling technique was used to recruit the health workers. Each participant was administered a socio-demographic questionnaire, the ILO/ICN/WHO/PSI Workplace Violence Questionnaire and the 12- item General Health Questionnaire. A total of 380 health workers were recruited, with a mean age of 36.4 (±7.64) years. The prevalence rates of workplace violence and risk of psychiatric morbidity were 39.9% and 38.5%. Factors with independent associations with workplace violence included young age, female sex and worry about workplace violence while a widowed, separated or divorced marital status and being victim of workplace violence independently increased risk for developing psychiatric morbidity. This study therefore showed that workplace violence is common in the health care setting, and significant proportion of workers are at risk for developing psychiatric morbidity. These observations suggest need for the regular mental health screening of health workers, as well as the need for programmes aimed at preventing workplace violence in this setting