Epidemiology of eating disorders part III: Social epidemiology and case definitions revisited.

Accepted manuscript version. Published version at <a href=http://doi.org/10.1080/21662630.2015.1022197>http://doi.org/10.1080/21662630.2015.1022197</a>.The previous papers in this series outlined a historical panorama and presented updated knowledge about putative risk factors and how eating disorders are distributed in various populations. In this final paper, we discuss in what way comorbidity findings and transdiagnostic issues may change our conceptions about ‘an epidemiological case’ from the current definition of eating disorders based on the recent version of the Diagnostic and Statistical Manual of Mental Disorders (i.e. the DSM-5), and to what extent an alternative definition may introduce new perspectives of prevention. The paper also provides an update on issues relevant for treatment dissemination

The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells-1

<p><b>Copyright information:</b></p><p>Taken from "The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells"</p><p>http://www.biomedical-engineering-online.com/content/6/1/33</p><p>BioMedical Engineering OnLine 2007;6():33-33.</p><p>Published online 6 Sep 2007</p><p>PMCID:PMC2018722.</p><p></p>aces. GAPDH was used as housekeeping gene and served to normalize the results. Agarose gels are 0 standard error of these five experiments. * p < 0.05 vs. PS control

The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells-3

<p><b>Copyright information:</b></p><p>Taken from "The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells"</p><p>http://www.biomedical-engineering-online.com/content/6/1/33</p><p>BioMedical Engineering OnLine 2007;6():33-33.</p><p>Published online 6 Sep 2007</p><p>PMCID:PMC2018722.</p><p></p>aces. GAPDH was used as housekeeping gene and served to normalize the results. Agarose gels are representative of 5 experiments. Quantitative results are the mean ± standard error of these five experiments. * p < 0.05 vs. PS control

Effect of human (short) Link-N on CD canine chondrocyte-like cells (CLCs).

<p>GAG and DNA content (mean + SD) and histological evaluation of CD canine CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β₁, 1 μg/mL or 10 ng/mL human Link-N (LN), or 0.5 μg/mL or 5 ng/mL human short Link-N (sLN) for 28 days in normoxia (21% O₂). (<b>a</b>) GAG content, (<b>b</b>) DNA content, (<b>c</b>) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (<b>d</b>) total amount of GAGs released in the culture medium, (<b>e</b>) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. xx,xxx: significantly different (<i>p</i> < 0.01 and <i>p</i> < 0.001 respectively) from all other conditions. <i>n</i> = 6 (<i>in duplicates</i>).</p

Alignment of human, bovine, and canine (short) Link-N peptides.

<p>Alignment of human, bovine, and canine (short) Link-N peptides.</p

The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells-0

<p><b>Copyright information:</b></p><p>Taken from "The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells"</p><p>http://www.biomedical-engineering-online.com/content/6/1/33</p><p>BioMedical Engineering OnLine 2007;6():33-33.</p><p>Published online 6 Sep 2007</p><p>PMCID:PMC2018722.</p><p></p>aces. GAPDH was used as housekeeping gene and served to normalize the results. Agarose gels are representative of 5 experiments. Quantitative results are the mean ± standard error of these five experiments. * p < 0.05 vs. PS control

The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells-2

<p><b>Copyright information:</b></p><p>Taken from "The effect of novel nitrogen-rich plasma polymer coatings on the phenotypic profile of notochordal cells"</p><p>http://www.biomedical-engineering-online.com/content/6/1/33</p><p>BioMedical Engineering OnLine 2007;6():33-33.</p><p>Published online 6 Sep 2007</p><p>PMCID:PMC2018722.</p><p></p>aces. GAPDH was used as housekeeping gene and served to normalize the results. Agarose gels are representative of 5 experiments. Quantitative results are the mean ± standard error of these five experiments. * p < 0.05 vs. PS control

Effect of human (short) Link-N on bovine chondrocyte-like cells (CLCs).

<p>GAG and DNA content (mean + SD) and histological evaluation of bovine CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β₁, 1 μg/mL or 10 ng/mL human Link-N (LN), or 0.5 μg/mL or 5 ng/mL human short Link-N (sLN) for 28 days in normoxia (21% O₂). (<b>a</b>) GAG content, (<b>b</b>) DNA content, (<b>c</b>) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (<b>d</b>) total amount of GAGs released in the culture medium, (<b>e</b>) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. ● and ○: significantly different (<i>p</i> < 0.05) from all other conditions except for the bars with the same symbol; x: significantly different (<i>p</i> < 0.05) from all other conditions. <i>n</i> = 6 (<i>in duplicates</i>).</p

Effect of canine (short) Link-N on bovine chondrocyte-like cells (CLCs).

<p>GAG and DNA content (mean + SD) and histological evaluation of bovine CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β₁, 1 μg/mL or 10 ng/mL canine Link-N (LN), or 0.5 μg/mL or 5 ng/mL canine short Link-N (sLN) for 28 days in hypoxia (5% O₂). (<b>a</b>) GAG content, (<b>b</b>) DNA content, (<b>c</b>) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (<b>d</b>) total amount of GAGs released in the culture medium, (<b>e</b>) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. *: <i>p</i> < 0.05; xx: significantly different (<i>p</i> < 0.01) from all other conditions. <i>n</i> = 6 (<i>in duplicates</i>).</p

Effect of canine (short) Link-N on human chondrocyte-like cells (CLCs).

<p>GAG and DNA content (mean + SD) and histological evaluation of human CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β₁, 1 μg/mL or 10 ng/mL canine Link-N (LN), or 0.5 μg/mL or 5 ng/mL canine short Link-N (sLN) for 28 days in hypoxia (5% O₂). (<b>a</b>) GAG content, (<b>b</b>) DNA content, (<b>c</b>) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (<b>d</b>) total amount of GAGs released in the culture medium, (<b>e</b>) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. *: <i>p</i> < 0.05. <i>n</i> = 3 (<i>in duplicates</i>).</p