11 research outputs found

    The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma

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    Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression

    Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein

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    Tristetraprolin (TTP) is a RNA-binding protein that inhibits the expression of pro-inflammatory cytokines and invasiveness-associated genes. TTP levels are decreased in many different cancer types and it has been proposed that this protein could be used as a prognostic factor in breast cancer. Here, using publicly available DNA microarray datasets, “serial analysis of gene expression” libraries and qRT-PCR analysis, we determined that TTP mRNA is present in normal breast cells and its levels are significantly decreased in all breast cancer subtypes. In addition, by immunostaining, we found that TTP expression is higher in normal breast tissue and benign lesions than in infiltrating carcinomas. Among these, lower grade tumors showed increased TTP expression compared to higher grade cancers. Therefore, these data indicate that TTP protein levels would provide a better negative correlation with breast cancer invasiveness than TTP transcript levels. In mice, we found that TTP mRNA and protein expression is also diminished in mammary tumors. Interestingly, a strong positive association of TTP expression and mammary differentiation was identified in normal and tumor cells. In fact, TTP expression is highly increased during lactation, showing good correlation with various mammary differentiation factors. TTP expression was also induced in mammary HC11 cells treated with lactogenic hormones, mainly by prolactin, through Stat5A activation. The effect of this hormone was highly dependent on mammary differentiation status, as prolactin was unable to elicit a similar response in proliferating or neoplastic mammary cells. In summary, these studies show that TTP expression is strongly linked to the mammary differentiation program in human and mice, suggesting that this protein might play specific and relevant roles in the normal physiology of the gland.Facultad de Ciencias MĂ©dicasCentro de Investigaciones InmunolĂłgicas BĂĄsicas y Aplicada

    Expression of heme oxygenase-1 in non small cell lung cancer (NSCLC) and its correlation with clinical data

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    While changes in heme oxygenase (HO-1) in lung cancer have already been reported, conflicting results were obtained for enzyme expression in human lung cancer specimens. Therefore, the aim of this work was to study HO-1 expression in a large collection of human lung cancer samples. For this purpose, we analyzed the expression of HO-1 in an organized tissue microarray (TMA) and investigated its correlation with clinicopathological data. Ninety-six percent of tumor samples were positive for HO-1, and the expression of HO-1 was significantly higher in cancerous than in non-cancerous tissues. Importantly, HO-1 expression correlated with advanced stages and lymph node involvement. Additionally, quantitative RT-PCR in 18 pairs of human lung carcinomas and their adjacent non-malignant tissues was performed. Our results demonstrate that HO-1 protein is upregulated in epithelial malignant cells in NSCLC and its expression is associated with higher stages of the disease. Additionally, different subcellular localization is observed between tumor and adjacent non-malignant tissues.Fil: Degese, MarĂ­a Sol. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de FisiologĂ­a, BiologĂ­a Molecular y Celular. Laboratorio de FisiologĂ­a y BiologĂ­a Molecular; Argentina. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias; ArgentinaFil: MendizĂĄbal, Javier Enrique. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de FisiologĂ­a, BiologĂ­a Molecular y Celular. Laboratorio de FisiologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - BahĂ­a Blanca. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca. Universidad Nacional del Sur. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca; ArgentinaFil: Gutkind, J. Silvio. National Institutes of Health; Estados UnidosFil: Molinolo, Alfredo. National Institutes of Health; Estados UnidosFil: Hewitt, Stephen M.. National Institutes of Health; Estados UnidosFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - BahĂ­a Blanca. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca. Universidad Nacional del Sur. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de FisiologĂ­a, BiologĂ­a Molecular y Celular. Laboratorio de FisiologĂ­a y BiologĂ­a Molecular; ArgentinaFil: Coso, Omar Adrian. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de FisiologĂ­a, BiologĂ­a Molecular y Neurociencias; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas. Centro CientĂ­fico TecnolĂłgico Conicet - BahĂ­a Blanca. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca. Universidad Nacional del Sur. Instituto de Investigaciones BioquĂ­micas de BahĂ­a Blanca; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de FisiologĂ­a, BiologĂ­a Molecular y Celular. Laboratorio de FisiologĂ­a y BiologĂ­a Molecular; Argentin

    Current State of History of Psychology Teaching and Education in Argentina: An Empirical Bibliometric Investigation

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    This study is an empirical analysis of the field’s current state in Argentinian universities. Bibliometric parameters were used to retrieve the total listed texts (N = 797) of eight undergraduate history courses’ syllabi from Argentina’s most populated public university psychology programs. Then, professors in charge of the selected courses (N = 7) were interviewed regarding the structure, procedure, and instructional strategies of their courses. Results showed predominance of works produced by Argentinian scholars and secondary sources. Syllabi showed limited incorporation of primary sources, recent research papers, and certain psychological schools. The pervasive presence of infrastructural constraints and lecture-based instruction are seen as limitations that should be collectively debated and addressed by courses’ faculty.Fil: Fierro, Catriel. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de PsicologĂ­a; ArgentinaFil: Ostrovsky, Ana Elisa. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de PsicologĂ­a; ArgentinaFil: Di DomĂ©nico, Maria Cristina. Consejo Nacional de Investigaciones CientĂ­ficas y TĂ©cnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de PsicologĂ­a; Argentin

    Solid-phase Chemistry: A Useful Tool to Discover Modulators of Protein Interactions

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