STUDIO DELLE MODIFICAZIONI MORFOLOGICHE CORNEALI CON MICROSCOPIA CONFOCALE IN VIVO DOPO IONTOFORESI CROSS-LINKING TRANSEPITELIALE

Lo studio svolto indaga le modificazioni ultrastrutturali corneali dopo un trattamento di cross-linking (CXL) corneale transepiteliale mediante iontoforesi in pazienti affetti da cheratocono. Gli accertamenti clinici e strumentali effettuati nel post-operatorio, dimostrando la sicurezza del trattamento utilizzato, l'arresto della progressione della patologia e le modificazioni istologiche dei vari strati corneali. La microscopia corneale confocale in vivo e la tomografia a coerenza ottica del segmento anteriore (OCT) hanno permesso inoltre di studiare, nel periodo postoperatorio, l'effettiva profondità di azione del trattamento di CXL, nel contesto corneale

Water-Soluble Nitroxyl Porphyrin Complexes FeIITPPSHNO and FeIITPPSNO- Obtained from Isolated FeIITPPSNO·

The first biomimetic water soluble FeII-porphyrin nitroxyl complexes were obtained and characterized by UV-Vis in protonated and deprotonated forms by reduction of previously isolated and characterized FeIITPPSNO?. The pKa involved in the FeII-HNO ⇄ FeII-NO− + H+ equilibrium was estimated to be around 9.7. The FeIITPPSHNO complex spontaneously reoxidizes to the nitrosyl form following a first order kinetic decay with a measured kinetic constant of k = 0.017 s-1. Experiments show that the HNO adduct undergoes unimolecular homolytic cleavage of the H-NO bond. DFT calculations suggest a phlorin radical intermediate for this reaction. The deprotonated NO− complex resulted more stable, with a half-life of about 10 minutes.Fil: Mazzeo, Agostina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Pellegrino, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Doctorovich, Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentin

Structural characterization of the water-soluble porphyrin complexes [FeII(TPPS) (NO•)]4─ and [μ-O-([FeIII(TPPS)])2]8─

Iron water-soluble porphyrins have been long used as biomimetic compounds for modelling the active sites found in heme-enzymes. In this regard, the anionic porphyrin [FeIII(TPPS)]3─ and its coordination complexes have been repeatedly chosen as suitable water-soluble platforms for bioinorganic chemistry studies. In this work we report for the first time the crystal structure of the water-soluble nitrosyl complex [FeII(TPPS) (NO•)]4─ along with that of oxodimeric ferric species [μ-O-([FeIII(TPPS)])2]8─.Fil: Mazzeo, Agostina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Gaviglio, Carina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área Investigaciones y Aplicaciones no Nucleares; ArgentinaFil: Pellegrino, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Doctorovich, Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentin

Structural characterization of the water-soluble porphyrin complexes [FeII(TPPS) (NO•)]4─ and [μ-O-([FeIII(TPPS)])2]8─

Iron water-soluble porphyrins have been long used as biomimetic compounds for modelling the active sites found in heme-enzymes. In this regard, the anionic porphyrin [FeIII(TPPS)]3─ and its coordination complexes have been repeatedly chosen as suitable water-soluble platforms for bioinorganic chemistry studies. In this work we report for the first time the crystal structure of the water-soluble nitrosyl complex [FeII(TPPS) (NO•)]4─ along with that of oxodimeric ferric species [μ-O-([FeIII(TPPS)])2]8─.Fil: Mazzeo, Agostina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Gaviglio, Carina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia del Área Investigaciones y Aplicaciones no Nucleares; ArgentinaFil: Pellegrino, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Doctorovich, Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentin

Observations on midgut of Apis mellifera workers (Hymenoptera: Apoidea) under controlled acute exposures to a Bacillus thuringiensis-based biopesticide

International audienceAbstractMorphostructural investigations have been carried out on Apis mellifera workers treated with single controlled acute exposures to a biopesticide containing Bacillus thuringiensis (Bt), to detect midgut changes until 96 h. Our findings show concentration-dependence of these changes, reflecting in different degrees on both mortality and behaviour. In particular, some midgut changes are also found 96 h after treatment. Our results show that the tested product does not affect survival at presumable environmental concentrations, so confirming the lesser toxicity of Bt-based biopesticides compared to other pesticides. However, in the light of the discovered long-term changes, we discuss the opportunity of taking into account possible chronic exposures to Bt-based products on A. mellifera

Incidental diagnosis of lung adenocarcinoma following coronavirus OC 43 severe pneumonia

Viral infections are frequent among patients with thoracic malignancies prompted by dysregulation of innate and adaptative immune response. Clinical symptoms and radiological findings of both viral pneumonia and lung adenocarcinoma may overlap resulting in diagnostic and clinical challenges.We present the case of a women admitted to our department presenting with an acute manifestation of coronavirus OC43 pneumonia with underlying undiagnosed lung adenocarcinoma

Chemometric-assisted cocrystallization: Supervised pattern recognition for predicting the formation of new functional cocrystals

Owing to the antimicrobial and insecticide properties, the use of natural compounds like essential oils and their active components has proven to be an effective alternative to synthetic chemicals in different fields ranging from drug delivery to agriculture and from nutrition to food preservation. Their limited application due to the high volatility and scarce water solubility can be expanded by using crystal engineering approaches to tune some properties of the active molecule by combining it with a suitable partner molecule (coformer). However, the selection of coformers and the experimental effort required for discovering cocrystals are the bottleneck of cocrystal engineering. This study explores the use of chemometrics to aid the discovery of cocrystals of active ingredients suitable for various applications. Partial Least Squares–Discriminant Analysis is used to discern cocrystals from binary mixtures based on the molecular features of the coformers. For the first time, by including failed cocrystallization data and considering a variety of chemically diverse compounds, the proposed method resulted in a successful prediction rate of 85% for the test set in the model validation phase and of 74% for the external test set

Shaping white light through electroluminescent fully organic coupled microcavities.

A new approach to generate and manage white light from an organic light emitting diode is shown. It consists in the coupling of two microcavity resonators made of only metallic and organic layers, whose operation principle is analogous to that of two mechanical harmonic oscillators coupled by a spring . This approach allows the solution of key open points in the field of plastic white light source