Environmental complaint insights through text mining based on the driver, pressure, state, impact, and response (DPSIR) framework. Evidence from an Italian environmental agency

Individuals, local communities, environmental associations, private organizations, and public representatives and bodies may all be aggrieved by environmental problems concerning poor air quality, illegal waste disposal, water contamination, and general pollution. Environmental complaints represent the expressions of dissatisfaction with these issues. As the time-consuming of managing a large number of complaints, text mining may be useful for automatically extracting information on stakeholder priorities and concerns. The paper used text mining and semantic network analysis to crawl relevant keywords about environmental complaints from two online complaint submission systems: online claim submission system of Regional Agency for Prevention, Environment and Energy (Arpae) (“Contact Arpae”); and Arpae's internal platform for environmental pollution (“Environmental incident reporting portal”) in the Emilia-Romagna Region, Italy. We evaluated the total of 2477 records and classified this information based on the claim topic (air pollution, water pollution, noise pollution, waste, odor, soil, weather-climate, sea-coast, and electromagnetic radiation) and geographical distribution. Then, this paper used natural language processing to extract keywords from the dataset, and classified keywords ranking higher in Term Frequency-Inverse Document Frequency (TF-IDF) based on the driver, pressure, state, impact, and response (DPSIR) framework. This study provided a systemic approach to understanding the interaction between people and environment in different geographical contexts and builds sustainable and healthy communities. The results showed that most complaints are from the public and associated with air pollution and odor. Factories (particularly foundries and ceramic industries) and farms are identified as the drivers of environmental issues. Citizen believed that environmental issues mainly affect human well-being. Moreover, the keywords of “odor”, “report”, “request”, “presence”, “municipality”, and “hours” were the most influential and meaningful concepts, as demonstrated by their high degree and betweenness centrality values. Keywords connecting odor (classified as impacts) and air pollution (classified as state) were the most important (such as “odor-burnt plastic” and “odor-acrid”). Complainants perceived odor annoyance as a primary environmental concern, possibly related to two main drivers: “odor-factory” and “odors-farms”. The proposed approach has several theoretical and practical implications: text mining may quickly and efficiently address citizen needs, providing the basis toward automating (even partially) the complaint process; and the DPSIR framework might support the planning and organization of information and the identification of stakeholder concerns and priorities, as well as metrics and indicators for their assessment. Therefore, integration of the DPSIR framework with the text mining of environmental complaints might generate a comprehensive environmental knowledge base as a prerequisite for a wider exploitation of analysis to support decision-making processes and environmental management activities

The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague Dawley rats: study design and first in-life endpoints evaluation.

BACKGROUND: Glyphosate-based herbicides (GBHs) are the most widely used pesticides worldwide, and glyphosate is the active ingredient of such herbicides, including the formulation known as Roundup. The massive and increasing use of GBHs results in not only the global burden of occupational exposures, but also increased exposure to the general population. The current pilot study represents the first phase of a long-term investigation of GBHs that we are conducting over the next 5 years. In this paper, we present the study design, the first evaluation of in vivo parameters and the determination of glyphosate and its major metabolite aminomethylphosphonic acid (AMPA) in urine. METHODS: We exposed Sprague-Dawley (SD) rats orally via drinking water to a dose of glyphosate equivalent to the United States Acceptable Daily Intake (US ADI) of 1.75 mg/kg bw/day, defined as the chronic Reference Dose (cRfD) determined by the US EPA, starting from prenatal life, i.e. gestational day (GD) 6 of their mothers. One cohort was continuously dosed until sexual maturity (6-week cohort) and another cohort was continuously dosed until adulthood (13-week cohort). Here we present data on general toxicity and urinary concentrations of glyphosate and its major metabolite AMPA. RESULTS: Survival, body weight, food and water consumption of the animals were not affected by the treatment with either glyphosate or Roundup. The concentration of both glyphosate and AMPA detected in the urine of SD rats treated with glyphosate were comparable to that observed in animals treated with Roundup, with an increase in relation to the duration of treatment. The majority of glyphosate was excreted unchanged. Urinary levels of the parent compound, glyphosate, were around 100-fold higher than the level of its metabolite, AMPA. CONCLUSIONS: Glyphosate concentrations in urine showed that most part of the administered dose was excreted as unchanged parent compound upon glyphosate and Roundup exposure, with an increasing pattern of glyphosate excreted in urine in relation to the duration of treatment. The adjuvants and the other substances present in Roundup did not seem to exert a major effect on the absorption and excretion of glyphosate. Our results demonstrate that urinary glyphosate is a more relevant marker of exposure than AMPA in the rodent model

Reproductive and developmental toxicity study using Sprague-Dawley rats exposed under various calendars to the weedkiller Glyphosate and commercial formulations Glyphosate-based

Glyphosate-based herbicides (GBHs) are the most globally used herbicides raising the risk of environmental exposition. Carcinogenic effects are only one component of the multiple adverse health effects of Glyphosate and GBHs that have been reported. Questions related to hazards and corresponding risks identified in relation to endocrine disrupting effects are rising. The present study investigated the possible reproductive/developmental toxicity of GBHs administered to male and female Sprague-Dawley rats under various calendar of treatment. Assessments included maternal and reproductive outcome of F0 and F1 dams exposed to GBHs throughout pregnancy and lactation and developmental landmarks and sexual characteristics of offspring. The study was designed in two stages. In the first stage Glyphosate, or its commercial formulation Roundup Bioflow, was administered to rats at the dose of 1.75 mg/kg bw/day (Glyphosate US Acceptable Daily Intake) from the prenatal period until adulthood. In the second stage, multiple toxicological parameters were simultaneously assessed, including multigeneration reproductive/developmental toxicity of Glyphosate and two GBHs (Roundup Bioflow and Ranger Pro). Man-equivalent doses, beginning from 0.5 mg/kg bw/day (ADI Europe) up to 50 mg/kg bw/day (NOAEL Glyphosate), were administered to male and female rats, covering specific windows of biological susceptibility. The results of stage 1 and preliminary data from stage 2 experiments characterize GBHs as probable endocrine disruptors as suggested by: 1) androgen-like effects of Roundup Bioflow, including a significant increase of anogenital distances in both males and females, delay of first estrous and increased testosterone in females; 2) slight puberty onset anticipation in the high dose of Ranger Pro group, observed in the F1 generation treated from in utero life until adulthood; 3) a delayed balano-preputial separation achievement in the high dose of Ranger Pro-treated males exposed only during the peri-pubertal period, indicating a direct and specific effect of GBHs depending on the timing of exposure

FISHing with Rat Sperm; the effect of glyphosate toxicity on sex chromosome aneuploidy in rats

Background: Glyphosate (N-(Phosphonomethyl-glycine)) is one of the most frequently used chemicals in herbicides worldwide; and is ubiquitous in the environment and food supply. Recent studies have identified glyphosate as a potential endocrine disrupting chemical (EDC). EDCs have been associated with a decrease in testicular function, including increases in aneuploidy, an abnormal number of chromosomes in sperm cells. The presence of aneuploidy in sperm cells can contribute to early embryo loss as well as children with Klinefelter and Turner syndromes. This study investigates the impact of glyphosate in its chemical form and in the form of the commercial herbicide Roundup®, on testicular function. Methods: Triple-probe fluorescence in situ hybridization (FISH) is used to identify sex chromosome disomy (XX, YY, XY) in sperm cells. The FISH method being developed for this study is based on three peer-reviewed studies using rat sperm FISH to ascertain aneuploidy, and our current lab protocol for human sperm FISH. For methods development we used 14 samples of epididymal sperm from 8 non-study rats extracted in differing volumes of sodium citrate buffer. Our protocol includes removal and separation of the sperm tail for enhanced imaging, swelling of the nucleus and denaturation of the DNA to provide ideal conditions for hybridization of custom-made probes for chromosomes X, Y and an autosomal control. The probes are enumerated using an adaptation of the human sperm procedure for imaging and nuclei are scored semi-automatically using a Leica DMi8 inverted fluorescent microscope and specialized analysis software. To investigate the effects of glyphosate, we collected 54 epididymal sperm samples from rats in three groups; (I) no treatment, (II) treatment with glyphosate, (III) treatment with Roundup®. Animals in treatment groups were dosed through drinking water at the acceptable daily intake for humans in the USA, 1.75mg per kilogram of body weight per day. Conclusions: Preliminary data on aneuploidy frequencies, specifically XX, XY, YY, and total disomy, and comparisons between dosed and non-dosed animals will be described and observations about chemical impacts on sperm abnormalities will be provided

Evaluation of Toxicant-Associated Fatty Liver Disease and Liver Neoplastic Progress in Sprague-Dawley Rats Treated with Low Doses of Aflatoxin B1 Alone or in Combination with Extremely Low Frequency Electromagnetic Fields

The term toxicant-associated fatty liver disease (TAFLD) has been proposed to describe fatty liver diseases connected to toxicants other than alcohol. Aflatoxins are mycotoxins commonly found as contaminants in foods and feeds, which are known liver toxicants and potential candidates as potential causes of TAFLD. Aflatoxin B1 (AFB1) was administered at low doses to Sprague-Dawley (SD) rats, alone or in combination with S-50 Hz an extremely low frequency electromagnetic field (ELFEMF), to study the evolution of TAFLD, preneoplastic and neoplastic lesions of the liver and the potential enhancing effect of lifespan exposure to ELFEMF. Steatosis, inflammation and foci of different types were significantly increased in both aflatoxin-treated males and females, which is consistent with a pattern of TAFLD. A significant increase in adenomas, cystic dilation of biliary ducts, hepatocellular hyperplasia and hypertrophy and oval cell hyperplasia were also observed in treated females only. The administration of low doses of AFB1 caused TAFLD in SD rats, inducing liver lesions encompassing fatty infiltration, foci of different types and adenomas. Furthermore, the pattern of change observed in preneoplastic liver lesions often included liver steatosis and steatohepatitis (TASH). ELFEMF did not result in any enhancing or toxic effect in the liver of SD rats

Control data on endocrine sensitive endpoints for untreated Sprague-Dawley rats from the Ramazzini Institute colony

Background and aim: Findings from laboratory animals as well as human studies suggest that Endocrine Disrupting Chemicals (EDCs) cause a number of reproductive health outcomes. Rats have been used extensively for developmental and reproductive physiology and endocrinology research and a number of endocrine sensitive endpoints have been well established in a variety of regulatory guidelines on rodent bioassays. We monitored the background data on some endocrine sensitive endpoints for untreated Sprague- Dawley rats from the Cesare Maltoni Cancer Research of the Ramazzini Institute colony (SD-CMCRC/RI). Materials and methods: General reproductive indices from dams and data for the entire litter were recorded. All the littermates were retained until the achievement of puberty and balanopreputial separation (BPS) was monitored in all the males; estrous cycle length and pattern were also evaluated in one female/litter. We compared our data with those provided by the Health and Environmental Sciences Institute (HESI) of the International Life Sciences Institute (ILSI). Results: Overall, reproductive indices and pre-post weaning litter data of SD-CMCRC/RI rats were comparable with those reported by ILSI. Conclusions: Procedures for monitoring and physiological biological variations in our SD-CMCRC/RI rats fall within the range of values typically obtained for the selected endpoints. Further investigations are suggested in order to verify whether retaining all pups to sexual maturation can improve the sensitivity to discriminate between natural variation and treatment effects. A more comprehensive analysis of other relevant endocrine sensitive endpoints should be performed in order to provide a representation of the normal developmental landmarks and endocrine values at different ages

Exploring occupational toxicant exposures in patients with metabolic dysfunction-associated steatotic liver disease: A prospective pilot study

Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) has been traditionally associated with insulin resistance and obesity. Recently, pollutants have been shown to contribute to the development of MASLD. Given the global burden of MASLD, understanding whether pollutants are merely associated with steatosis or contribute to its progression to advanced chronic liver disease (ACLD) and hepatocellular carcinoma (HCC) is critical. Workers exposed to occupational toxicants represent an ideal population for assessing the potentially hazardous consequences of professional exposure. Confirming a link between occupational exposure and ACLD/HCC may not only provide further elements in understanding MASLD, but also contribute to preventive strategies for exposed workers. Objective: This study aimed to assess the prevalence of self-reported occupational exposure to toxicants in patients with MASLD. Methods: This hospital-based prospective pilot study included 201 patients with MASLD. Data on workplace toxicant exposure were collected systematically using a structured questionnaire. Subsequently, patients with ACLD and/or HCC (n = 55) were compared to controls (n = 146). Logistic regression analysis and propensity score models were used to investigate the associations between self-reported occupational exposure and ACLD and/or HCC. Results: Patients with ACLD/HCC reported exposure to metals, halogenated refrigerants, pain/resins, and fuel emissions more often than the controls. After controlling for confounders, durations of 21-30 years and >30 years of occupational exposure to toxicants showed odds ratios (ORs) of 2.31 (95 % confidence interval [CI]: 1.09-4.88, p = 0.029) and 4.47 (95 % CI: 2.57-7.78, p<0.001), respectively. Conclusions: In this pilot study, patients with MASLD complications were more likely to report workplace toxicant exposure. Our results warrant future multicentre confirmatory studies, as implementing prevention policies may reduce the risk of life-threatening diseases among exposed populations