4 research outputs found
Toxicology of Environmental Samples From the Perspective of Biodefense
The ability of the recombinant bacterial SWITCH-Test to recognise agents possibly
used for terroristic attacks was investigated. For this purpose genotoxic and
cytotoxic dose dependent response of S. typhimurium to 30 environmental
samples provided from the University of Pécs, Hungary was determined. In
addition to these samples, reference samples were used for validation of the test.
These mainly consist of solutions containing metals, such as arsenic, cadmium,
mercury, and sodium acide. Furthermore, the toxicity of the cyanobacterial toxin
Microcystin-LR was an example for a biological toxin is determined.
The results were used to show that the SWITCH-Test can be a valuable tool for
the determination of genotoxic and cytotoxic potentials of environmental samples,
after the possible release of dangerous chemicals by terrorists. In this connection
it was shown that the test is able to detect a bioterrorist assault with chemicals
immediately and fast. This would be a prerequisite for a rapid evacuation of the
population and evolvement of an alertness strategy. With the example of
Microcystin-LR the effect, of an easy prepareable and cheap toxin that is probable
to be used by terrorists, is shown.
On the basis of these experiments dose responce relationships for the genotoxic
and cytotoxic response were established. From these, two samples were identified
as genotoxic and nine as cytotoxic. The reference samples with known constituent
concentrations revealed the SWITCH-Test to be a sensitive for tool for such
applications