1,245 research outputs found
Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
<p>Abstract</p> <p>Background</p> <p>Carbonic anhydrase (CA) of the chicken has attracted attention for a long time because it has an important role in the eggshell formation. The developmental profile of CA-II isozyme levels in chicken erythrocytes has not been determined or reported. Furthermore, the relations with CA-II in erythrocyte and egg production are not discussed. In the present study, we isolated CA-II from erythrocytes of chickens and determined age-related changes of CA-II levels in erythrocytes.</p> <p>Methods</p> <p>Chicken CA-II was purified by a combination of column chromatography. The levels of CA-II in the hemolysate of the chicken were determined using the ELISA system in blood samples from 279 female chickens, ages 1 to 93 weeks, 69 male chickens, ages 3 to 59 weeks and 52 weeks female Araucana-chickens.</p> <p>Results</p> <p>The mean concentration of CA-II in hemolysate from 1-week-old female was 50.8 ± 11.9 mg/g of Hb. The mean levels of CA-II in 25-week-old (188.1 ± 82.6 mg/g of Hb), 31-week-old (193.6 ± 69.7 mg/g of Hb) and 49-week-old (203.8 ± 123.5 mg/g of Hb) female-chickens showed the highest level of CA-II. The levels of CA-II in female WL-chickens significantly decreased at 63 week (139.0 ± 19.3 mg/g of Hb). The levels of CA-II in female WL-chicken did not change from week 63 until week 93.The mean level of CA-II in hemolysate of 3-week-old male WL-chickens was 78.3 ± 20.7 mg/g of Hb. The levels of CA-II in male WL-chickens did not show changes in the week 3 to week 59 timeframe. The mean level of CA-II in 53-week-old female Araucana-chickens was 23.4 ± 1.78 mg/g of Hb. These levels of CA-II were about 11% of those of 49-week-old female WL-chickens. Simple linear regression analysis showed significant associations between the level of CA-II and egg laying rate from 16 week-old at 63 week-old WL-chicken (p < 0.01).</p> <p>Conclusions</p> <p>Developmental changes and sexual differences of CA-II concentration in WL-chicken erythrocytes were observed. The concentration of CA-II in the erythrocyte of WL-chicken was much higher than that in Araucana-chicken (p < 0.01).</p
Production of antihydrogen at reduced magnetic field for anti-atom trapping
We have demonstrated production of antihydrogen in a 1T solenoidal
magnetic field. This field strength is significantly smaller than that used in
the first generation experiments ATHENA (3T) and ATRAP (5T). The
motivation for using a smaller magnetic field is to facilitate trapping of
antihydrogen atoms in a neutral atom trap surrounding the production region. We
report the results of measurements with the ALPHA (Antihydrogen Laser PHysics
Apparatus) device, which can capture and cool antiprotons at 3T, and then
mix the antiprotons with positrons at 1T. We infer antihydrogen production
from the time structure of antiproton annihilations during mixing, using mixing
with heated positrons as the null experiment, as demonstrated in ATHENA.
Implications for antihydrogen trapping are discussed
Evidence For The Production Of Slow Antiprotonic Hydrogen In Vacuum
We present evidence showing how antiprotonic hydrogen, the quasistable
antiproton-proton (pbar-p) bound system, has been synthesized following the
interaction of antiprotons with the hydrogen molecular ion (H2+) in a nested
Penning trap environment. From a careful analysis of the spatial distributions
of antiproton annihilation events, evidence is presented for antiprotonic
hydrogen production with sub-eV kinetic energies in states around n=70, and
with low angular momenta. The slow antiprotonic hydrogen may be studied using
laser spectroscopic techniques.Comment: 5 pages with 4 figures. Published as Phys. Rev. Letters 97, 153401
(2006), in slightly different for
Three Dimensional Annihilation Imaging of Antiprotons in a Penning Trap
We demonstrate three-dimensional annihilation imaging of antiprotons trapped
in a Penning trap. Exploiting unusual feature of antiparticles, we investigate
a previously unexplored regime in particle transport; the proximity of the trap
wall. Particle loss on the wall, the final step of radial transport, is
observed to be highly non-uniform, both radially and azimuthally. These
observations have considerable implications for the production and detection of
antihydrogen atoms.Comment: Invited Talk at NNP03, Workshop on Non-Neutral Plasmas, 200
A novel antiproton radial diagnostic based on octupole induced ballistic loss
We report results from a novel diagnostic that probes the outer radial
profile of trapped antiproton clouds. The diagnostic allows us to determine the
profile by monitoring the time-history of antiproton losses that occur as an
octupole field in the antiproton confinement region is increased. We show
several examples of how this diagnostic helps us to understand the radial
dynamics of antiprotons in normal and nested Penning-Malmberg traps. Better
understanding of these dynamics may aid current attempts to trap antihydrogen
atoms
Positron plasma diagnostics and temperature control for antihydrogen production
Production of antihydrogen atoms by mixing antiprotons with a cold, confined,
positron plasma depends critically on parameters such as the plasma density and
temperature. We discuss non-destructive measurements, based on a novel,
real-time analysis of excited, low-order plasma modes, that provide
comprehensive characterization of the positron plasma in the ATHENA
antihydrogen apparatus. The plasma length, radius, density, and total particle
number are obtained. Measurement and control of plasma temperature variations,
and the application to antihydrogen production experiments are discussed.Comment: 5 pages, 4 figures, to be published in Phys. Rev. Let
Detection of antihydrogen annihilations with a Si-micro-strip and pure CsI detector
In 2002, the ATHENA collaboration reported the creation and detection of cold
(~15 K) antihydrogen atoms [1]. The observation was based on the complete
reconstruction of antihydrogen annihilations, simultaneous and spatially
correlated annihilations of an antiproton and a positron. Annihilation
byproducts are measured with a cylindrically symmetric detector system
consisting of two layers of double sided Si-micro-strip modules that are
surrounded by 16 rows of 12 pure CsI crystals (13 x 17.5 x 17 mm^3). This paper
gives a brief overview of the experiment, the detector system, and event
reconstruction.
Reference 1. M. Amoretti et al., Nature 419, 456 (2002).Comment: 7 pages, 5 figures; Proceedings for the 8th ICATPP Conference on
Astroparticle, Particle, Space Physics, Detectors and Medical Physics
Applications (Como, Italy October 2003) to be published by World Scientific
(style file included
ATHENA -- First Production of Cold Antihydrogen and Beyond
Atomic systems of antiparticles are the laboratories of choice for tests of
CPT symmetry with antimatter. The ATHENA experiment was the first to report the
production of copious amounts of cold antihydrogen in 2002. This article
reviews some of the insights that have since been gained concerning the
antihydrogen production process as well as the external and internal properties
of the produced anti-atoms. Furthermore, the implications of those results on
future prospects of symmetry tests with antimatter are discussed.Comment: Proc. of the Third Meeting on CPT and Lorentz Symmetry, Bloomington
(Indiana), USA, August 2004, edited by V. A. Kostelecky (World Scientific,
Singapore). 10 pages, 5 figures, 1 table. Author affiliations cor
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