NMRI Measurements of Flow of Granular Mixtures

We investigate complex 3D behavior of granular mixtures in shaking and shearing devices. NMRI can non-invasively measure concentration, velocity, and velocity fluctuations of flows of suitable particles. We investigate origins of wall-shear induced convection flow of single component particles by measuring the flow and fluctuating motion of particles near rough boundaries. We also investigate if a mixture of different size particles segregate into their own species under the influence of external shaking and shearing disturbances. These non-invasive measurements will reveal true nature of convecting flow properties and wall disturbance. For experiments in a reduced gravity environment, we will design a light weight NMR imager. The proof of principle development will prepare for the construction of a complete spaceborne system to perform experiments in space

LATS1/2 kinases trigger self-renewal of cancer stem cells in aggressive oral cancer

Cancer stem cells (CSCs), which play important roles in tumor initiation and progression, are resistant to many types of therapies. However, the regulatory mechanisms underlying CSC-specific properties, including self-renewal, are poorly understood. Here, we found that LATS1/2, the core Hippo pathway-kinases, were highly expressed in the oral squamous cell carcinoma line SAS, which exhibits high capacity of CSCs, and that depletion of these kinases prevented SAS cells from forming spheres under serum-free conditions. Detailed examination of the expression and activation of LATS kinases and related proteins over a time course of sphere formation revealed that LATS1/2 were more highly expressed and markedly activated before initiation of self-renewal. Moreover, TAZ, SNAIL, CHK1/2, and Aurora-A were expressed in hierarchical, oscillating patterns during sphere formation, suggesting that the process consists of four sequential steps. Our results indicate that LATS1/2 trigger self-renewal of CSCs by regulating the Hippo pathway, the EMT, and cell division

FOREVER22: the first bright galaxies with population III stars at redshifts z≃10−20z \simeq 10-20 and comparisons with JWST data

We study the formation of the first galaxies in overdense regions modelled by the FORmation and EVolution of galaxies in Extremely overdense Regions motivated by SSA22 (FOREVER22) simulation project. Our simulations successfully reproduce the star formation rates and the $M_{\rm UV}-M_{\rm star}$ relations of candidate galaxies at $z \sim 10-14$ observed by the James Webb Space Telescope (JWST). We suggest that the observed galaxies are hosted by dark-matter haloes with $M_{\rm h} \gtrsim 10^{10}~{\rm M_{\odot}}$ and are in short-period starburst phases. On the other hand, even simulated massive galaxies in overdense regions cannot reproduce the intense star formation rates and the large stellar masses of observed candidates at $z \sim 16$. Also, we show that the contribution of population III stars to the UV flux decreases as the stellar mass increases and it is a few percent for galaxies with $M_{\rm star} \sim 10^{7}~{\rm M_{\odot}}$. Therefore, a part of the observed flux by JWST could be the light from population III stars. Our simulations suggest that the UV flux can be dominated by population III stars and the UV-slope shows $\beta \lesssim -3$ if future observations would reach galaxies with $M_{\rm stars} \sim 10^{5}~{\rm M_{\odot}}$ at $z \sim 20$ of which the mass fraction of population III stars can be greater than 10 percent.Comment: 9 pages, 6 figures, accepted for publication in MNRA

A nucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression

<p>Abstract</p> <p>Background</p> <p>A novel anticancer drug 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd, TAS106) has been shown to radiosensitize tumor cells and to improve the therapeutic efficiency of X-irradiation. However, the effect of TAS106 on cellular DNA repair capacity has not been elucidated. Our aim in this study was to examine whether TAS106 modified the repair capacity of DNA double-strand breaks (DSBs) in tumor cells.</p> <p>Methods</p> <p>Various cultured cell lines treated with TAS106 were irradiated and then survival fraction was examined by the clonogenic survival assays. Repair of sublethal damage (SLD), which indicates DSBs repair capacity, was measured as an increase of surviving cells after split dose irradiation with an interval of incubation. To assess the effect of TAS106 on the DSBs repair activity, the time courses of γ-H2AX and 53BP1 foci formation were examined by using immunocytochemistry. The expression of DNA-repair-related proteins was also examined by Western blot analysis and semi-quantitative RT-PCR analysis.</p> <p>Results</p> <p>In clonogenic survival assays, pretreatment of TAS106 showed radiosensitizing effects in various cell lines. TAS106 inhibited SLD repair and delayed the disappearance of γ-H2AX and 53BP1 foci, suggesting that DSB repair occurred in A549 cells. Western blot analysis demonstrated that TAS106 down-regulated the expression of BRCA2 and Rad51, which are known as keys among DNA repair proteins in the homologous recombination (HR) pathway. Although a significant radiosensitizing effect of TAS106 was observed in the parental V79 cells, pretreatment with TAS106 did not induce any radiosensitizing effects in BRCA2-deficient V-C8 cells.</p> <p>Conclusions</p> <p>Our results indicate that TAS106 induces the down-regulation of BRCA2 and the subsequent abrogation of the HR pathway, leading to a radiosensitizing effect. Therefore, this study suggests that inhibition of the HR pathway may be useful to improve the therapeutic efficiency of radiotherapy for solid tumors.</p

噛ミング30学習による小学生の咀嚼の習慣と口腔内状態に関する介入研究

The aim of this study was to investigate the relationship between chewing behavior and oral conditions in elementary school children based on an intervention study. The subjects were allocated into an intervention group (5th grade students, n=81) and a control group (5th grade students, n=39) in 2 elementary schools in Tokushima Prefecture, Japan. Eating habits were self-reported using a questionnaire in both groups. The PMA index (Schour-Massler index) of each subject in the intervention group was assessed. The school lunch program “Chewing 30” was performed 5 times per year using a chewing counter. After the completion of the program, a significant difference in behavior change regarding “sufficient chewing” was observed between the intervention and control groups (p<0.01). The participants in the intervention group were sub-divided into 2 groups based on the change of “sufficient chewing”. The PMA index of the non-improved group significantly increased (p<0.05), while that of the improved group showed no significant difference. These results suggest an association between insufficient chewing behavior and gingival inflammation in elementary school children. The program “Chewing 30” might be effective to prevent gingival inflammation in addition to promoting sufficient chewing.　本研究では，小学生の咀嚼習慣と口腔内状態との関連性を介入研究にて調査することを目的とした． 　徳島県内にある2つの小学校のうち，1校の5年生（81名）を介入群とし，他校の5年生（39名）をコントロール群として，食習慣に関する保健調査を実施した．介入群では，児童それぞれのPMA index（Schour-Massler index）を評価した．さらに，1年間を通して計5回学校給食時に咀嚼計を用いた「噛ミング30学習」を実施した． 　介入終了後，“よく嚙む” 項目においてコントロール群と介入群では有意な差が認められた（p<0.01）．“よく嚙む” 項目の変化の有無により，介入群を2つのグループに分類した．“よく嚙む” 項目の非改善群ではPMA index中央値が有意に増加していた（p<0.05）が，改善群ではPMA index中央値の増加は認められなかった． 　これらの結果は，小学生において，不十分な咀嚼習慣と歯肉の炎症に関連性があることを示している．「噛ミング30学習」は，よく嚙むことを促すだけでなく，歯肉炎の予防にも効果がある可能性が示唆された

Inactivation of ESBL-E. coli

The prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing rapidly and spreading worldwide, particularly in Asia, compared to other regions. In the last ten years, in our hospital, in particular, there has been a < 30% increase. To prevent the spread of ESBL in hospitals and the community, the ultraviolet (UV) A-light-emitting diode (LED) irradiation device was used to inactivate ESBL-E. coli in human livestock and the environment. ESBL-E. coli and E. coli bacterial samples were collected from patients at Tokushima University Hospital (Tokushima City, Japan). The UVA-LED irradiation system had 365 nm single wavelength, and the current of the circuit was set to 0.23 or 0.50 A consistently. Results demonstrated that UVA-LED was useful for the inactivation of ESBL-E. coli and E. coli. The minimum energy dosage required to inactivate ESBL-E. coli and E. coli was 40.76 J/cm2 (45 min) in the first type of UVA-LED and 38.85 J/cm2 (5 min) in the second type. There were no significant differences between ESBL-E. coli and E. coli. The inactivation of ESBL-E. coli was dependent on energy. These findings suggest that UVA-LED with 365 nm single wavelength could be useful for surface decontamination in healthcare facilities

EMPRESS. XI. SDSS and JWST Search for Local and z~4-5 Extremely Metal-Poor Galaxies (EMPGs): Clustering and Chemical Properties of Local EMPGs

We search for local extremely metal-poor galaxies (EMPGs), selecting photometric candidates by broadband color excess and machine-learning techniques with the SDSS photometric data. After removing stellar contaminants by shallow spectroscopy with Seimei and Nayuta telescopes, we confirm that three candidates are EMPGs with 0.05--0.1 $Z_\odot$ by deep Magellan/MagE spectroscopy for faint {\sc[Oiii]}$\lambda$4363 lines. Using a statistical sample consisting of 105 spectroscopically-confirmed EMPGs taken from our study and the literature, we calculate cross-correlation function (CCF) of the EMPGs and all SDSS galaxies to quantify environments of EMPGs. Comparing another CCF of all SDSS galaxies and comparison SDSS galaxies in the same stellar mass range ($10^{7.0}-10^{8.4} M_\odot$), we find no significant ($>1\sigma$) difference between these two CCFs. We also compare mass-metallicity relations (MZRs) of the EMPGs and those of galaxies at $z\sim$ 0--4 with a steady chemical evolution model and find that the EMPG MZR is comparable with the model prediction on average. These clustering and chemical properties of EMPGs are explained by a scenario of stochastic metal-poor gas accretion on metal-rich galaxies showing metal-poor star formation. Extending the broadband color-excess technique to a high-$z$ EMPG search, we select 17 candidates of $z\sim$ 4--5 EMPGs with the deep ($\simeq30$ mag) near-infrared JWST/NIRCam images obtained by ERO and ERS programs. We find galaxy candidates with negligible {\sc[Oiii]}$\lambda\lambda$4959,5007 emission weaker than the local EMPGs and known high-$z$ galaxies, suggesting that some of these candidates may fall in 0--0.01 $Z_\odot$, which potentially break the lowest metallicity limit known to date

EMPRESS. IX. Extremely Metal-Poor Galaxies are Very Gas-Rich Dispersion-Dominated Systems: Will JWST Witness Gaseous Turbulent High-z Primordial Galaxies?

We present kinematics of 6 local extremely metal-poor galaxies (EMPGs) with low metallicities ($0.016-0.098\ Z_{\odot}$) and low stellar masses ($10^{4.7}-10^{7.6} M_{\odot}$). Taking deep medium-high resolution ($R\sim7500$) integral-field spectra with 8.2-m Subaru, we resolve the small inner velocity gradients and dispersions of the EMPGs with H$\alpha$ emission. Carefully masking out sub-structures originated by inflow and/or outflow, we fit 3-dimensional disk models to the observed H$\alpha$ flux, velocity, and velocity-dispersion maps. All the EMPGs show rotational velocities ($v_{\rm rot}$) of 5--23 km s$^{-1}$ smaller than the velocity dispersions ($\sigma_{0}$) of 17--31 km s$^{-1}$, indicating dispersion-dominated ($v_{\rm rot}/\sigma_{0}=0.29-0.80<1$) systems affected by inflow and/or outflow. Except for two EMPGs with large uncertainties, we find that the EMPGs have very large gas-mass fractions of $f_{\rm gas}\simeq 0.9-1.0$. Comparing our results with other H$\alpha$ kinematics studies, we find that $v_{\rm rot}/\sigma_{0}$ decreases and $f_{\rm gas}$ increases with decreasing metallicity, decreasing stellar mass, and increasing specific star-formation rate. We also find that simulated high-$z$ ($z\sim 7$) forming galaxies have gas fractions and dynamics similar to the observed EMPGs. Our EMPG observations and the simulations suggest that primordial galaxies are gas-rich dispersion-dominated systems, which would be identified by the forthcoming James Webb Space Telescope (JWST) observations at $z\sim 7$.Comment: Submitted to ApJ; After revisio