Tracheal Stenosis Caused by Unnoticed Foreign Bodies

We describe an extremely rare case of tracheal stenosis caused by unnoticed microscopic fiber-like foreign bodies. A 66-year-old woman complained of dyspnea with inspiratory stridor. Magnifying electroendoscopy and computed tomography revealed stenosis involving the entire circumference of the tracheal lumen. Tracheotomy and biopsy were performed. Histologically, the lesion showed chronic inflammation with a deposition of fiber-like foreign bodies. The patient had no history of trauma or inhalation injury, but had undergone intratracheal intubation on 4 occasions. The lesion was incised using semiconductor laser photoresection, and the postoperative course was good. To the best of our knowledge, this represents the first report in the English literature of tracheal stenosis caused by unnoticed foreign bodies. The origin of these fiber-like foreign bodies remains unclear but might be related to chronic inflammation resulting from intratracheal intubations

Meteorological Observations at Syowa in 1972 by the 13th Japanese Antarctic Research Expedition

This paper describes the results of meteorological surface and aerological observations at Syowa Station from February 1, 1972 to February 10, 1973. The main subjects of observations, the method, and the equipments are the same as the previous year. Observed results (surface observation at 00, 06, 09, 12, 15, 18 GMT and aerological observation at 00 GMT) were sent to World Meteorological Centre in Melbourne via Mawson Station based on the WMO code, and were recorded according to the WMO regulations. Meteorological characters observed this year are as follows: 1) Temperature in the first half of this year was a little lower than the average but was unusualy high in August and September. 2) Snow accumulation in August and September was larger than in normal years, 3) The sunshine duration in the latter half was extremely shorter than in normal years, arid consequently, the thawing of snow was very slow. 4) Sudden warming in the lowci stratosphere was observed clearly since October 8. For instance, the isotherm of -50℃ was found to shift downward periodically, with a period of 11 or 12 days. 5) A rainbow was observed on the early moring of December 17. 1972. This was the first recoid ever since Syowa Station was opened. Moreover, we carried out observations of drifting snow, total amount of ozone, amount of evaporation, katabatic wind as well as atmospheric ozone, electricity and long wave radiation using a specially desinged radiosonde

Meteological Observations at Syowa Station in 1977 by the 18th Japanese Antarctic Research Expedition

This paper describes the results of routine observations of surface and aerological weather, total ozone measurement using Dobson's spectrophotometer and vertical distribution of ozone using special ozone radio sondes at Syowa Station (index number 89532) between 1 February 1977 and 31 January 1978. The main items of observation, method and equipments are the same as the previous year. The data of the surface and aerological observations were regularly reported to the World Meteorological Center in Melbourne via Mawson Station according to the WMO regulations

Organelle membrane-specific chemical labeling and dynamic imaging in living cells

狙った細胞内小器官脂質の可視化に成功 --オートファゴソーム形成機構解明に貢献--. 京都大学プレスリリース. 2020-09-28.Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking

ダイ13ジ ナンキョク チイキ カンソクタイ キショウ ブモン ホウコク 1972

This paper describes the results of meteorological surface and aerological observations at Syowa Station from February 1, 1972 to February 10, 1973. The main subjects of observations, the method, and the equipments are the same as the previous year. Observed results (surface observation at 00, 06, 09, 12, 15, 18 GMT and aerological observation at 00 GMT) were sent to World Meteorological Centre in Melbourne via Mawson Station based on the WMO code, and were recorded according to the WMO regulations. Meteorological characters observed this year are as follows: 1) Temperature in the first half of this year was a little lower than the average but was unusualy high in August and September. 2) Snow accumulation in August and September was larger than in normal years, 3) The sunshine duration in the latter half was extremely shorter than in normal years, arid consequently, the thawing of snow was very slow. 4) Sudden warming in the lowci stratosphere was observed clearly since October 8. For instance, the isotherm of -50℃ was found to shift downward periodically, with a period of 11 or 12 days. 5) A rainbow was observed on the early moring of December 17. 1972. This was the first recoid ever since Syowa Station was opened. Moreover, we carried out observations of drifting snow, total amount of ozone, amount of evaporation, katabatic wind as well as atmospheric ozone, electricity and long wave radiation using a specially desinged radiosonde

Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide

細胞内の狙った天然タンパク質を迅速に化学修飾する分子技術を開発 --不可逆阻害剤開発のための新しい戦略--. 京都大学プレスリリース. 2018-05-16.Selective modification of native proteins in live cells is one of the central challenges in recent chemical biology. As a unique bioorthogonal approach, ligand-directed chemistry recently emerged, but the slow kinetics limits its scope. Here we successfully overcome this obstacle using N-acyl-N-alkyl sulfonamide as a reactive group. Quantitative kinetic analyses reveal that ligand-directed N-acyl-N-alkyl sulfonamide chemistry allows for rapid modification of a lysine residue proximal to the ligand binding site of a target protein, with a rate constant of ~104 M−1 s−1, comparable to the fastest bioorthogonal chemistry. Despite some off-target reactions, this method can selectively label both intracellular and membrane-bound endogenous proteins. Moreover, the unique reactivity of N-acyl-N-alkyl sulfonamide enables the rational design of a lysine-targeted covalent inhibitor that shows durable suppression of the activity of Hsp90 in cancer cells. This work provides possibilities to extend the covalent inhibition approach that is currently being reassessed in drug discovery

A Set of Organelle-Localizable Reactive Molecules for Mitochondrial Chemical Proteomics in Living Cells and Brain Tissues

Protein functions are tightly regulated by their subcellular localization in live cells, and quantitative evaluation of dynamically altered proteomes in each organelle should provide valuable information. Here, we describe a novel method for organelle-focused chemical proteomics using spatially limited reactions. In this work, mitochondria-localizable reactive molecules (MRMs) were designed that penetrate biomembranes and spontaneously concentrate in mitochondria, where protein labeling is facilitated by the condensation effect. The combination of this selective labeling and liquid chromatography–mass spectrometry (LC–MS) based proteomics technology facilitated identification of mitochondrial proteomes and the profile of the intrinsic reactivity of amino acids tethered to proteins expressed in live cultured cells, primary neurons and brain slices. Furthermore, quantitative profiling of mitochondrial proteins whose expression levels change significantly during an oxidant-induced apoptotic process was performed by combination of this MRMs-based method with a standard quantitative MS technique (SILAC: stable isotope labeling by amino acids in cell culture). The use of a set of MRMs represents a powerful tool for chemical proteomics to elucidate mitochondria-associated biological events and diseases