Seleção De Espécies E Procedências De Pinus Para Região De Assis, Estado De São Paulo

The objective of the study was the selection of Pinus species and provenances for the region of Assis, state of São Paulo. The species and provenance test was established at the Assis State Forest, using the randomized blocks design with treatments represented by species (Pinus oocarpa, P. maximinoi and P. tecunumanii) and provenances of the species, four blocks and square plots with 49 plants (7 × 7), at a spacing of 3 × 2.5 m. At 21 years after planting the traits were measured: diameter at breast height (DAP), total height (ALT), individual real volume (VOL) and the survival rate (SOBRE). Significant differences were observed between species and provenances of different species (p< 0.01) for SOBRE, indicating the possibility of selection among species and provenances. The genetic divergence between species varied between growth traits from zero to 0.141, and was 0.683 for SOBRE, indicating that most of the genetic variation is distributed within species for growth traits and between species for SOBRE. Genetic and phenotypic significant and positive correlations were observed between the DAP and VOL (minimum 0.926; p< 0.01) indicating that selection for the DAP will result in an increase in the VOL. The results indicate that there are genetic variations among Pinus species and provenances of different species for SOBRE and P. oocarpa is most suitable for commercial reforestation in environmental conditions of Assis.4411167568

Parâmetros genéticos e análise de trilha para o florescimento precoce e características agronômicas da alface

O objetivo deste trabalho foi avaliar os parâmetros genéticos das características agronômicas e de tolerância ao florescimento precoce de onze cultivares de alface, bem como verificar a existência de associação entre as características. O experimento foi realizado em ambiente protegido, em delineamento de blocos ao acaso, com quatro repetições e doze plantas por parcela. Quarenta e cinco dias após o transplantio das mudas, foram mensuradas as seguintes características: massa de matéria fresca total e "comercial" da parte aérea, massa de matéria seca "comercial" da parte aérea, massa de matéria fresca e seca da raiz, diâmetro e circunferência da cabeça, altura de planta, número de folhas por planta e número de dias até a antese. Há variabilidade genética entre as cultivares, em todas as variáveis, exceto quanto à circunferência de planta e matéria fresca da raiz. As cultivares Regina 500, Lívia e Atração foram superiores quanto ao número de dias para o florescimento e também para as demais características avaliadas. A seleção contra o florescimento precoce ocasionou ganho em todas as características; porém, não interferiu na matéria seca da raiz. A matéria fresca da parte aérea e o diâmetro de cabeça são indicadas para a seleção indireta contra o florescimento precoce

Development And Characterization Of 14 Microsatellite Loci From An Enriched Genomic Library Of Eucalyptus Camaldulensis Dehnh

Eucalyptus camaldulensis Dehnh is an Australian tree species which occurs in various climatic and environmental conditions and show large genetic diversity. Twenty five microsatellite markers were developed from a CT 8-GT 8 enriched genomic library of E. camaldulensis. The number of alleles ranged from 4 to 13 (average of 8). The polymorphism information content (PIC) and the discriminating power (D) of each primer ranged from 0.37 to 0.88 (average of 0.72) and 0.48 to 0.99 (average of 0.84), respectively. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.25 to 0.90, respectively. Four loci showed statistically significant from Hardy-Weinberg equilibrium after Bonferroni correction (P, (5%) &lt; 0.0038). All polymorphic markers were tested for cross-amplification in 25 different Eucalyptus species. Those microsatellite loci will be useful to investigate questions of genetic diversity and structure, gene flow, mating system and ex situ genetic conservation of E. camaldulensis. © Springer Science+Business Media B.V. 2009.11465469Billotte, N., Lagoda, P.J.R., Risterucci, A.M., Baurens, F.C., Microsatellite enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Brondani, R.P.V., Brondani, C., Tarchini, R., Grattapaglia, D., Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and (1998) Urophylla Theor Appl Genet, 97, pp. 816-827Butcher, P.A., Otero, A., McDonald, M.W., Moran, G.F., Nuclear RFLP variation in Eucalyptus camaldulensis Dehnh from northern Australia (2002) Heredity, 88, pp. 402-412Butcher, P.A., McDonald, M.W., Bell, J.C., Congruence between environmental parameters, morphology and genetic structure in Australia's most widely distributed eucalypt, Eucalyptus camaldulensis (2009) Tree Genet Genomes, 5, pp. 189-210Cordeiro, G.M., Taylor, G.O., Henry, R.J., Characterization of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploidy species (2000) Plant Sci, 155, pp. 161-168Creste, S., Tulmann Neto, A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol Biol Rep, 19, pp. 299-306Dexter, B.D., Rose, H.J., Davies, N., River regulation and associate forest management problems in the River Murray red gum forests (1986) Aust For, 49, pp. 16-27Don, R.H., Cox, P.T., Wainwright, B.J., Baker, K., Mattick, J.S., "Touchdown" PCR to circumvent spurious priming during gene amplification (1991) Nucleic Acids Res, 19, p. 4008Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus, 12, pp. 13-15Eldridge, K., Davidson, J., Harwood, C., Wyk, G., (1993) Eucalypt Domestication and Breeding, pp. 60-71. , Clarendon, OxfordGoudet, J., FSTAT (Version 2.9.3.2.): A computer program to calculate F-statistics (1995) J Hered, 86, pp. 485-486Kalinowski, S.T., Taper, M.L., Marshall, T.C., Revising how computer program Cervus accommodates genotyping error increase success in paternity assignment (2007) Mol Ecol, 16, pp. 1099-1106Lewis, P., Zaykin, D., Genetic data analysis (GDA): Computer program for the analysis of allelic data (software) (2002) Version 1.1, , http://alleyn.eeb.uconn.edu/gda/Nieto, V.M., Rodriguez, J., Eucalyptus camaldulensis Dehnh (2003) Tropical Tree Seed Manual. Part II-species Descriptions, pp. 466-467. , In: Vozzo JA (ed), United States Department of Agriculture Forest Service, WashingtonPryor, L.D., (1976) Biology of Eucalyptus, , Esward Arnold, LondonTemnykh, S., Declerck, G., Lukashova, A., Lipovich, L., Catinhour, S., McCouch, S., Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential (2001) Genome, 11, pp. 1441-1452Tessier, C., David, J., This, P., Boursiquot, J.M., Charrier, A., Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L (1999) Theor Appl Genet, 98, pp. 171-17