New host plant records for Oenomaus ortygnus (Cramer) (Lepidoptera: Lycaenidae) in Mexico

This is the first record of Oenomaus ortygnus (Cramer) damaging fruits of ilama (Annona diversifolia) and extends the butterfly distribution for three states in Mexico.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Pró-Reitoria de Pesquisa of the Universidade de São Paulo (USP

Micropropagación del lirio amazónico (Eucharis grandiflora Planch. & Linden) mediante organogénesis directa.

Amazon lily (Eucharis grandiflora) is a species whose characteristics offer enormous potential for ornamental use, so we sought to have an efficient in vitro protocol for both obtaining and multiplying seedlings. We used the inorganic salts of the basic medium of Murashige & Skoog (1962) at 100%, supplemented with 0.40 mg·L-1 thiamine, 100 mg·L-1 inositol, 80 mg·L-1 L-cysteine, and 30 g·L-1 sucrose. In the in vitro multiplication of seedlings, different levels of BAP were evaluated at doses of 0.0, 0.1, 0.3, 1.0, and 3.0 mg·L-1. To promote better shoot development, the best responses were observed in media without the presence of plant growth regulators and by evaluating sucrose content at concentrations from 30 to 70 g·L-1. For seedling rooting, different doses of IAA, IBA, and NAA were used, in intervals of 0.0, 0.3, and 1.0 mg·L-1. For the acclimatization phase of seedlings obtained in vitro, they were placed in 1 L containers with peat moss. The results obtained in the phase of the establishment of explants, percentages of 30% of contamination were obtained. During the multiplication of seedlings, the use of BAP with a dose of 3.0 mg·L-1 produced a maximum of 3.8 shoots per explant. The best development of bulblets was obtained with 50 mg·L-1 of sucrose. During rooting, IAA and IBA did not produce significantly more roots than the control, although a greater length of roots was observed. Also, the addition of NAA promoted the formation of a greater number of roots and less vigor. During acclimatization in the greenhouse, 100% survival of seedlings at 50 days was achieved.El lirio amazónico (Eucharis grandiflora) es una especie cuyas características ofrecen un enorme potencial de uso ornamental, por lo que se buscó contar con un protocolo in vitro eficiente tanto para la obtención y multiplicación de plántulas. Se emplearon las sales inorgánicas del medio básico de Murashige & Skoog (1962) al 100%, suplementadas con 0.40 mg·L-1 de tiamina, 100 mg·L-1 de inositol, 80 mg·L-1 de L-cisteína, y 30 g·L-1 de sacarosa. En la multiplicación in vitro de plántulas, se evaluaron diferentes niveles de BAP en dosis de 0.0, 0.1, 0.3, 1.0 y 3.0 mg·L-1. Para el enraizamiento de plántulas, se emplearon diferentes dosis de AIA, AIB y ANA, en intervalos de 0.0, 0.3 y 1.0 mg·L-1. Para promover un mejor desarrollo de los brotes, las mejores respuestas se observaron en medios sin la presencia de reguladores del crecimiento y evaluando el contenido de sacarosa en concentraciones de 30 a 70 g·L-1. Para la fase de aclimatización de plántulas obtenidas in vitro, fueron colocadas en contenedores de 1 L con turba de musgo. En lo resultados obtenidos en la fase de establecimiento de explantes, se obtuvieron porcentajes del 30% de contaminación. Durante la multiplicación de plántulas, el uso de BAP con una dosis de 3.0 mg·L-1 produjo un máximo de 3.8 brotes por explante. El mejor desarrollo de los bulbillos se obtuvo con 50 g·L-1 de sacarosa. Durante el enraizamiento el AIA y AIB no produjeron significativamente más raíces que el testigo, aunque se observó una mayor longitud de éstas. Además, la adición del ANA promovió la formación de un mayor número de raíces, y un menor vigor. Durante la aclimatización en invernadero, se logró un 100% de sobrevivencia de plántulas producidas a los 50 días

EVALUACIÓN DEL EFECTO DE CADMIO SOBRE LA GERMINACIÓN Y ELONGACIÓN RADICAL DE SEMILLAS BACTERIZADAS DE AXONOPUS AFFINIS Y FESTUCA RUBRA

There has been reported different indexto biomonitoring the phytotoxic effects ofmetals in plants; one of them is based on the germination and plant lets growth, eventhough the root promotion is a major marker which measures the beneficial effect ofplant growth promoting rhizobacteria. Thepresent study analyzed the bacterizationeffect on germination and root elongationof Axonopus affinis (Chase) and Festucarubra L., as protection and promotingplant growth mechanisms at the expositionto a heavy metal. The seeds bacterizationwith the rhizobacteria Pseudomonas sp.Sp7D of both species, showed its promoting potential in the germination and theplantlets grown as its protecting potentialin cadmium presence for both species;even that A. affinis (Chase) showed too ahormetic response with cadmium and thetolerance to it.Se han reportado diferentes índices parabiomonitorear los efectos de fitotoxicidad demetales en plantas y uno de esos indicadoresestá basado en la germinación y desarrollode las plántulas; así mismo, la promoción delas raíces es uno de los principales marcadores con los que se mide el efecto benéfico delas rizobacterias promotoras del crecimientovegetal. El presente estudio analizó el efectode la bacterización sobre la germinación yelongación radical de Axonopus affinis (Chase) y Festuca rubra L., como mecanismosde protección y promoción del crecimientoante la exposición a un metal pesado.La bacterización de las semillas con la rizobacteria Pseudomonas sp. Sp7D en ambasespecies reflejó tanto su potencial promotordel crecimiento en la germinación y el crecimiento de las plántulas, donde éstos fueronmayores para Festuca rubra; como su potencial protector ante la presencia de cadmio(Cd) para ambas especies; no obstante queAxonopus affinis (Chase) mostró tambiénuna respuesta hormética ante la presenciadel metal y la tolerancia a éste

Analyzing multitarget activity landscapes using protein-ligand interaction fingerprints: interaction cliffs.

This is the original submitted version, before peer review. The final peer-reviewed version is available from ACS at http://pubs.acs.org/doi/abs/10.1021/ci500721x.Activity landscape modeling is mostly a descriptive technique that allows rationalizing continuous and discontinuous SARs. Nevertheless, the interpretation of some landscape features, especially of activity cliffs, is not straightforward. As the nature of activity cliffs depends on the ligand and the target, information regarding both should be included in the analysis. A specific way to include this information is using protein-ligand interaction fingerprints (IFPs). In this paper we report the activity landscape modeling of 507 ligand-kinase complexes (from the KLIFS database) including IFP, which facilitates the analysis and interpretation of activity cliffs. Here we introduce the structure-activity-interaction similarity (SAIS) maps that incorporate information on ligand-target contact similarity. We also introduce the concept of interaction cliffs defined as ligand-target complexes with high structural and interaction similarity but have a large potency difference of the ligands. Moreover, the information retrieved regarding the specific interaction allowed the identification of activity cliff hot spots, which help to rationalize activity cliffs from the target point of view. In general, the information provided by IFPs provides a structure-based understanding of some activity landscape features. This paper shows examples of analyses that can be carried out when IFPs are added to the activity landscape model.M-L is very grateful to CONACyT (No. 217442/312933) and the Cambridge Overseas Trust for funding. AB thanks Unilever for funding and the European Research Council for a Starting Grant (ERC-2013- StG-336159 MIXTURE). J.L.M-F. is grateful to the School of Chemistry, Department of Pharmacy of the National Autonomous University of Mexico (UNAM) for support. This work was supported by a scholarship from the Secretariat of Public Education and the Mexican government

Super-Radiant Dynamics, Doorways, and Resonances in Nuclei and Other Open Mesoscopic Systems

The phenomenon of super-radiance (Dicke effect, coherent spontaneous radiation by a gas of atoms coupled through the common radiation field) is well known in quantum optics. The review discusses similar physics that emerges in open and marginally stable quantum many-body systems. In the presence of open decay channels, the intrinsic states are coupled through the continuum. At sufficiently strong continuum coupling, the spectrum of resonances undergoes the restructuring with segregation of very broad super-radiant states and trapping of remaining long-lived compound states. The appropriate formalism describing this phenomenon is based on the Feshbach projection method and effective non-Hermitian Hamiltonian. A broader generalization is related to the idea of doorway states connecting quantum states of different structure. The method is explained in detail and the examples of applications are given to nuclear, atomic and particle physics. The interrelation of the collective dynamics through continuum and possible intrinsic many-body chaos is studied, including universal mesoscopic conductance fluctuations. The theory serves as a natural framework for general description of a quantum signal transmission through an open mesoscopic system.Comment: 85 pages, 10 figure

Diagnosis features of pediatric Gaucher disease patients in the era of enzymatic therapy, a national-base study from the Spanish Registry of Gaucher Disease

Background: The enzymatic replacement therapy (ERT) availability for Gaucher disease (GD) has changed the landscape of the disease, several countries have screening programs. These actions have promoted the early diagnosis and avoided many complications in pediatric patients. In Spain ERT has been available since 1993 and 386 patients have been included in the Spanish Registry of Gaucher Disease (SpRGD). The aim of this study is to analyze the impact of ERT on the characteristics at time of diagnosis and initial complications in pediatric Gaucher disease patients. Aim: To analyze the impact of ERT on the characteristics at time of diagnosis and initial complications in pediatric Gaucher disease patients. Methods: A review of data in SpRGD from patients'' diagnosed before 18 years old was performed. The cohort was split according the year of diagnosis (=1994, cohort A; =1995, cohort B). Results: A total of 98 pediatric patients were included, GD1: 80, GD3: 18; mean age: 7.2 (0.17-16.5) years, 58 (59.2%) males and 40 (40.8%) females. Forty-five were diagnosed = 1994 and 53 = 1995. Genotype: N370S/N370S: 2 (2.0%), N370S/L444P: 27 (27.5%), N370S/other: 47 (48%), L444P/L444P: 7 (7.1%), L444P/D409H: 2 (2.0%), L444P/other: 3 (6.2%), other/other: 10 (10.2%). The mean age at diagnosis was earlier in patients diagnosed after 1995 (p < 0.001) and different between the subtypes, GD1: 8.2 (0.2-16.5) years and GD3: 2.8 (0.17-10.2) years (p < 0.001). There were more severe patients in the group diagnosed before 1994 (p = 0.045) carrying L444P (2), D409H (2), G377S (1), G195W (1) or the recombinant mutation. The patients'' diagnosed =1994 showed worse cytopenias, higher chance of bone vascular complications at diagnosis and previous spleen removal. The patients started ERT at a median time after diagnosis of 5.2 years [cohort A] and 1.6 years [cohort B] (p < 0.001). Conclusions: The early diagnosis of Gaucher disease in the era of ERT availability has permitted to reduce the incidence of severe and irreversible initial complication in pediatric patients, and this has permitted better development of these patients. This is the largest pediatric cohort from a national registry

POTENCIAL PROBIÓTICO DE CEPAS DE BACILLUS CON PROPIEDADES ANTIMICROBIANAS FRENTE A PATÓGENOS DE PECES

El objetivo de este trabajo ha sido aislar, identificar y caracterizar cepas bacterianas procedentes de ambiente marino, fitoplancton, zooplancton, huevos, larvas de lenguado y diferentes juveniles (branquias, intestino, piel), que pudieran tener actividad probiótica y actividad antibacteriana frente a distintos patógenos de peces. Se aislaron un total de 217 cepas bacterianas. Tras un primer cribado mediante ensayos de inhibición en placa frente a patógenos, se seleccionaron 9 cepas y se identificaron utilizando la secuenciación de su rRNA 16S. Los resultados mostraron que las mejores candidatas eran cepas del género Bacillus. Varios de estas posibles cepas candidatas se caracterizaron in vitro para determinar su potencial probiótico, mediante ensayos de inhibición en co-cultivo con V. anguillarum, resistencia a bilis de pez, y adherencia a mucus intestinal de pez. Nuestros resultados mostraron que algunas de las cepas seleccionadas pertenecientes al género Bacillus, podrían ser consideradas como potenciales probióticos frente a patógenos de peces, por lo que el beneficio de la administración de estas cepas a cultivos de peces será posteriormente estudiada

Open and Hidden Charm Production in 920 GeV Proton-Nucleus Collisions

The HERA-B collaboration has studied the production of charmonium and open charm states in collisions of 920 GeV protons with wire targets of different materials. The acceptance of the HERA-B spectrometer covers negative values of xF up to xF=-0.3 and a broad range in transverse momentum from 0.0 to 4.8 GeV/c. The studies presented in this paper include J/psi differential distributions and the suppression of J/psi production in nuclear media. Furthermore, production cross sections and cross section ratios for open charm mesons are discussed.Comment: 5 pages, 9 figures, to be published in the proceedings of the 6th International Conference on Hyperons, Charm & Beauty Hadrons (BEACH04), Chicago, IL, June 27 - July 3, 200