246 research outputs found
BbrizAGL6 is differentially expressed during embryo sac formation of apomictic and sexual Brachiaria brizantha plants.
Evaluation of integrases in the control of gene regulation in mammalian cells: a tool for the construction of synthetic biological circuits.
PileLine: a toolbox to handle genome position information in next-generation sequencing studies
Exploring the proteomic profile of soybean bran: unlocking the potential for improving protein quality and quantity.
Na publicação: Liliane Marcia Mertz-Henning; Elibio Rech
Extraordinary room-temperature photoluminescence in WS2 monolayers
Individual monolayers of metal dichalcogenides are atomically thin
two-dimensional crystals with attractive physical properties different from
their bulk layered counterpart. Here we describe the direct synthesis of WS2
monolayers with triangular morphologies and strong room-temperature
photoluminescence (PL). Bulk WS2 does not present PL due to its indirect band
gap nature. The edges of these monolayers exhibit PL signals with extraordinary
intensity, around 25 times stronger than the platelets center. The structure
and composition of the platelet edges appear to be critical for the PL
enhancement effect. Electron diffraction revealed that platelets present zigzag
edges, while first-principles calculations indicate that sulfur-rich zigzag WS2
edges possess metallic edge states, which might tailor the optical response
reported here. These novel 2D nanoscale light sources could find diverse
applications including the fabrication of flexible/transparent/low-energy
optoelectronic devices
Depleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides.
In this study, we identified two uORFs in the 5'-UTR sequence of this gene. The entire 5' leader sequence was amplified, cloned, and site-directed mutated to delete its uORFs start codons (-ATG). The validation of these predicted uORFs was carried out in transformed Nicotiana benthamiana protoplasts using a dual-luciferase reporter vector, in which it is possible to analyze the effect of mutated and non-mutated uORFs by dividing the expression of luciferase by that of Renilla-luciferase (LUC/REN)
Corrosion Behavior of API 5L X80 Steel in the Produced Water of Onshore Oil Recovery Facilities
Regulated Nuclear Trafficking of rpL10A Mediated by NIK1 Represents a Defense Strategy of Plant Cells against Virus
The NSP-interacting kinase (NIK) receptor-mediated defense pathway has been identified recently as a virulence target of the geminivirus nuclear shuttle protein (NSP). However, the NIK1–NSP interaction does not fit into the elicitor–receptor model of resistance, and hence the molecular mechanism that links this antiviral response to receptor activation remains obscure. Here, we identified a ribosomal protein, rpL10A, as a specific partner and substrate of NIK1 that functions as an immediate downstream effector of NIK1-mediated response. Phosphorylation of cytosolic rpL10A by NIK1 redirects the protein to the nucleus where it may act to modulate viral infection. While ectopic expression of normal NIK1 or a hyperactive NIK1 mutant promotes the accumulation of phosphorylated rpL10A within the nuclei, an inactive NIK1 mutant fails to redirect the protein to the nuclei of co-transfected cells. Likewise, a mutant rpL10A defective for NIK1 phosphorylation is not redirected to the nucleus. Furthermore, loss of rpL10A function enhances susceptibility to geminivirus infection, resembling the phenotype of nik1 null alleles. We also provide evidence that geminivirus infection directly interferes with NIK1-mediated nuclear relocalization of rpL10A as a counterdefensive measure. However, the NIK1-mediated defense signaling neither activates RNA silencing nor promotes a hypersensitive response but inhibits plant growth and development. Although the virulence function of the particular geminivirus NSP studied here overcomes this layer of defense in Arabidopsis, the NIK1-mediated signaling response may be involved in restricting the host range of other viruses
Contributions of the old urban homegardens for food production and consumption in Rio Claro, Southeastern Brazil
- …