Changes in rumen capacity of dairy cows during the periparturient period

Four-ruminally fistulated, multiparous, pregnant Holstein cows were studied to characterize ruminal adaptations during the transition from gestation to lactation. Cows were fed typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM), and an alfalfa hay, corn silage based early lactation diet. Ruminal measurements were obtained 72 (late lactation), 51 (far-off dry), 23 and 9 (close-up dry) days before expected parturition and 6, 20, and 34 days postpartum. Measurements included total fill, dry matter fill, fluid fill, and water-holding capacity of the rumen. Dry matter intake and milk production data were collected daily and body weight and body condition were determined weekly. Body weights and condition increased during the dry period, whereas intake as a percentage of body weight decreased. Ruminal water holding capacity, an indicator of rumen capacity, increased linearly from late lactation to 34 days postpartum. These data suggest rumen capacity is not the causative factor of intake depression in dairy cows during the final 3 wk of gestation.; Dairy Day, 2001, Kansas State University, Manhattan, KS, 2001

Comlete lactational performance of cows fed wet corn gluten feed and pellet consisting of raw soybean hulls and corn steep liquor

We evaluated the effect of wet corn gluten feed and a novel product containing raw soybean hulls and corn steep liquor on performance in lactating dairy cows. Forty-six multiparous Holstein cows were used in a randomized incomplete block design. Cows were housed in tie stalls for the first 13 weeks of lactation and moved to group pens for the remainder of the study. Cows were blocked by calving date and assigned to control, wet corn gluten feed (20% of diet DM), or the novel product (20% of diet DM). Diets were administered as total mixed rations at the first feeding postpartum. Control contained (DM basis) 30% alfalfa hay, 15% corn silage, 32% corn, 9.3% whole cottonseed, 4.4% solvent soybean meal (SBM), 3.3% expeller SBM, 1.3% fish meal, 1% wet molasses, and 3.7% vitamins/minerals. Wet corn gluten feed replaced 10% alfalfa hay, 5% corn silage, 5% corn grain, and expeller SBM replaced solvent SBM to maintain diet rumen undegradable protein. The novel product replaced 10% alfalfa hay, 5% corn silage, 3% solvent SBM, and 2% corn. Diet crude protein % and energy density (Mcal/lb, NEL) for control, wet corn gluen feed, and the novel product were 18.4, 0.73; 18.2, 0.75; 18.5, 0.73; respectively. Milk, energy corrected milk, dry matter intake, and production efficiency (ratio of milk to DM intake) did not differ among diets during the first 91 days of lactation, but there was a diet by week interaction for production efficiency. Cows fed control were more efficient during the first 2 weeks postpartum than cows fed wet corn gluten feed and the novel product, likely due to increased fat mobilization from adipose tissue because intake as a percent of body weight was less for cows fed control. During weeks 3 through 14 postpartum, wet corn gluten feed and the novel product improved milk, energy corrected milk, and milk component yield, and production efficiency. Inclusion of wet corn gluten feed and the novel product at 20% of dietary DM as a partial replacement for alfalfa hay, corn silage, corn grain, and SBM in diets fed to lactating dairy cattle supported performance during early lactation and improved performance during mid and late lactation. In addition, combining wet corn gluten feed or the novel product with corn silage and alfalfa hay maintained milk fat yields, thereby demonstrating that they can serve as effective sources of fiber when fed at 20% of dietary DM. Improved performance attributed to wet corn gluten feed and the novel product is due to factors other than improved digestibility of the diets. These results indicate that wet corn gluten feed and the novel product can serve as alternative feedstuffs in diets fed to lactating dairy cattle.; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Relationship among concentrations of milk urea nitrogen and plasma urea nitrogen and feeding time

Eight Holstein cows were used to determine the relationship among milk urea nitrogen (MUN), plasma urea nitrogen (PUN), and feeding time. We first established that MUN concentrations were similar in concentration among quarters by comparing milk samples from each quarter just before milking. In order to determine if collecting a sample of milk from a quarter influenced the MUN in samples taken later, samples were obtained from the right front quarter (RF) at 2, 4, 6, and 8 hr after the AM milking and from the left front quarter (LF), right rear (RR), and left rear (LR) at 4, 6, and 8 h after the AM milking, respectively. The MUN in samples obtained from RF at 4 hr was lower (P\u3c0.01) than corresponding samples taken from LF, but samples from RF at 6 and 8 hr did not differ from corresponding samples obtained from RR and LR. We concluded that by 6 hr, the effect of previous milking on MUN concentration disappeared because of dilution. To determine the influence of feeding time on MUN concentrations, cows were fed half of their normal PM feeding, injected with oxytocin at the subsequent AM milking to reduce residual milk, and offered surplus feed after the AM milking. Milk samples were collected at 2, 4, 6, 8, 10, and 12 hr after feeding from RF, LF, RR, LR, RF, and LF quarters, respectively. Blood samples were obtained from the coccygeal vein at hourly intervals after feeding with the last sample collected 12 hr after feeding. The MUN concentrations at 2, 4, 6, and 8 hr were similar. The MUN at 10 hr was similar to those at 2 and 8 hr, less than that at 4 and 6 hr, and greater than that for the 12 hr sample. Concentrations of PUN peaked at 2 hr postfeeding, then gradually declined through 12 hr postfeeding. The MUN peaked at 6 hr postfeeding and then declined. Time after feeding significantly influenced PUN and MUN concentrations.; Dairy Day, 2000, Kansas State University, Manhattan, KS, 2000

Dolutegravir/Lamivudine Is Noninferior to Continuing Dolutegravir- and Non-Dolutegravir-Based Triple-Drug Antiretroviral Therapy in Virologically Suppressed People With Human Immunodeficiency Virus:DUALING Prospective Nationwide Matched Cohort Study

Background. Confirming the efficacy of dolutegravir/lamivudine in clinical practice solidifies recommendations on its use. Methods. Prospective cohort study (DUALING) in 24 human immunodeficiency virus (HIV) treatment centers in the Netherlands. HIV RNA–suppressed cases were on triple-drug antiretroviral regimens without prior virological failure or resistance and started dolutegravir/lamivudine. Cases were 1:2 matched to controls on triple-drug antiretroviral regimens by the use of dolutegravir-based regimens, age, sex, transmission route, CD4+ T-cell nadir, and HIV RNA zenith. The primary endpoint was the treatment failure rate in cases versus controls at 1 year by intention-to-treat and on-treatment analyses with 5% noninferiority margin. Results. The 2040 participants were 680 cases and 1380 controls. Treatment failure in the 390 dolutegravir-based cases versus controls occurred in 8.72% and 12.50% (difference: −3.78% [95% confidence interval {CI}, −7.49% to .08%]) by intention-to-treat and 1.39% and 0.80% (difference: 0.59% [95% CI, –.80% to 1.98%]) by on-treatment analyses. The treatment failure risk in 290 non-dolutegravir-based cases was also noninferior to controls. Antiretroviral regimen modifications unrelated to virological failure explained the higher treatment failure rate by intention-to-treat. A shorter time on triple-drug antiretroviral therapy and being of non-Western origin was associated with treatment failure. Treatment failure, defined as 2 consecutive HIV RNA &gt;50 copies/mL, occurred in 4 cases and 5 controls but without genotypic resistance detected. Viral blips occured comparable in cases and controls but cases gained more weight, especially when tenofovir-based regimens were discontinued. Conclusions. In routine care, dolutegravir/lamivudine was noninferior to continuing triple-drug antiretroviral regimens after 1 year, supporting the use of dolutegravir/lamivudine in clinical practice.</p

Dolutegravir/Lamivudine Is Noninferior to Continuing Dolutegravir- and Non-Dolutegravir-Based Triple-Drug Antiretroviral Therapy in Virologically Suppressed People With Human Immunodeficiency Virus:DUALING Prospective Nationwide Matched Cohort Study

Background. Confirming the efficacy of dolutegravir/lamivudine in clinical practice solidifies recommendations on its use. Methods. Prospective cohort study (DUALING) in 24 human immunodeficiency virus (HIV) treatment centers in the Netherlands. HIV RNA–suppressed cases were on triple-drug antiretroviral regimens without prior virological failure or resistance and started dolutegravir/lamivudine. Cases were 1:2 matched to controls on triple-drug antiretroviral regimens by the use of dolutegravir-based regimens, age, sex, transmission route, CD4+ T-cell nadir, and HIV RNA zenith. The primary endpoint was the treatment failure rate in cases versus controls at 1 year by intention-to-treat and on-treatment analyses with 5% noninferiority margin. Results. The 2040 participants were 680 cases and 1380 controls. Treatment failure in the 390 dolutegravir-based cases versus controls occurred in 8.72% and 12.50% (difference: −3.78% [95% confidence interval {CI}, −7.49% to .08%]) by intention-to-treat and 1.39% and 0.80% (difference: 0.59% [95% CI, –.80% to 1.98%]) by on-treatment analyses. The treatment failure risk in 290 non-dolutegravir-based cases was also noninferior to controls. Antiretroviral regimen modifications unrelated to virological failure explained the higher treatment failure rate by intention-to-treat. A shorter time on triple-drug antiretroviral therapy and being of non-Western origin was associated with treatment failure. Treatment failure, defined as 2 consecutive HIV RNA &gt;50 copies/mL, occurred in 4 cases and 5 controls but without genotypic resistance detected. Viral blips occured comparable in cases and controls but cases gained more weight, especially when tenofovir-based regimens were discontinued. Conclusions. In routine care, dolutegravir/lamivudine was noninferior to continuing triple-drug antiretroviral regimens after 1 year, supporting the use of dolutegravir/lamivudine in clinical practice.</p

Performance of holstein cows fed wet corn gluten feed or soyhull-steep liquor pellets during early lactation

Wet corn gluten feed and soyhull-steep liquor pellets tended to increase dry matter intake and milk yield. Combining condensed corn steep liquor with raw soybean hulls provides a convenient source of digestible fiber and rumen degradable protein

Metabolic changes during he transition period

We used four ruminally fistulated, multiparous, pregnant Holstein cows to measure changes in concentrations of plasma metabolite as the dairy cow transitions from one lactation to the next. Diets consisted of typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM), and an alfalfa hay-corn silage based early lactation diet. Calculated NEL (Mcal/lb), measured crude protein (%), and diet digestibilities (%; based on steers fed at 2% of BW) were 0.78, 18.7, 74.1; 0.70, 11.5, 66.2; 0.74, 15.6, 71.0; 0.73, 18.4, 70.7 for late lactation, far-off dry, close-up dry, and early lactation diets, respectively. Blood samples were obtained on day 79 prior to calving and weekly thereafter until calving and on days 1, 3, 5, 7, 15, 20, 25, 30, 60, and 90 after calving. Cows gained body weight and condition during the dry period, peaked just prior to calving, and lost weight and condition steadily through the first 11 weeks of lactation. Calculated energy balance was negative during the first 3 weeks of lactation. Plasma concentrations of non-esterified fatty acids (NEFA), glucose, and insulin to glucagon ratio remained fairly stable during the dry period. Plasma glucose increased just before calving, decreased markedly during early lactation, then increased and stabilized by day 30 of lactation. Plasma NEFA concentrations increased at calving and were elevated during early lactation, then returned to prepartum concentrations by day 30 of lactation. The insulin to glucagon ratio decreased just prior to calving, continued to decrease until day 7 of lactation, and then remained stable until the end of the trial. Changes in diet and intake affected plasma urea nitrogen, which decreased as dietary protein decreased during the far-off period, decreased with intake during the close-up period, and increased after calving consistent with the higher dietary protein and increase in dry matter intake. Most of the observed metabolic adaptations reflected the energy status of the cow with large shifts occurring around parturition. Certainly, some of the hormones associated with calving can initiate metabolic events favorable to lactation, but the changes in energy balance and nutrient supply support the continued diversion of nutrients to the mammary gland. These data support the concept that dairy cows experience a period of increased tissue mobilization from approximately 2 days prior to calving until 30 days after calving. In conclusion, a number of metabolic adaptations occur in transition dairy cows that provide clues to improve feeding and management guidelines

Relationship among concentrations of milk urea nitrogen and plasma urea nitrogen and feeding time

Eight Holstein cows were used to determine the relationship among milk urea nitrogen (MUN), plasma urea nitrogen (PUN), and feeding time. We first established that MUN concentrations were similar in concentration among quarters by comparing milk samples from each quarter just before milking. In order to determine if collecting a sample of milk from a quarter influenced the MUN in samples taken later, samples were obtained from the right front quarter (RF) at 2, 4, 6, and 8 hr after the AM milking and from the left front quarter (LF), right rear (RR), and left rear (LR) at 4, 6, and 8 h after the AM milking, respectively. The MUN in samples obtained from RF at 4 hr was lower (P<0.01) than corresponding samples taken from LF, but samples from RF at 6 and 8 hr did not differ from corresponding samples obtained from RR and LR. We concluded that by 6 hr, the effect of previous milking on MUN concentration disappeared because of dilution. To determine the influence of feeding time on MUN concentrations, cows were fed half of their normal PM feeding, injected with oxytocin at the subsequent AM milking to reduce residual milk, and offered surplus feed after the AM milking. Milk samples were collected at 2, 4, 6, 8, 10, and 12 hr after feeding from RF, LF, RR, LR, RF, and LF quarters, respectively. Blood samples were obtained from the coccygeal vein at hourly intervals after feeding with the last sample collected 12 hr after feeding. The MUN concentrations at 2, 4, 6, and 8 hr were similar. The MUN at 10 hr was similar to those at 2 and 8 hr, less than that at 4 and 6 hr, and greater than that for the 12 hr sample. Concentrations of PUN peaked at 2 hr postfeeding, then gradually declined through 12 hr postfeeding. The MUN peaked at 6 hr postfeeding and then declined. Time after feeding significantly influenced PUN and MUN concentrations

Dietary Soybean Lecithin Enhances Growth Performance, Feed Utilization Efficiency and Body Composition of Early Juvenile Milkfish, Chanos chanos

The study was conducted to evaluate the influence of dietary soybean lecithin (SBL) on the growth performance, feed utilization, and survival of early juvenile milkfish. Five experimental diets containing increasing levels of SBL (0 g, 0.75 g, 1.5 g, 3.0 g and 6.0 g/100 g diet) were formulated and fed to triplicate groups of early juvenile milkfish (≈ 9.0 mg) for 50 days. Results showed that dietary lecithin inclusion improved Weight Gain (WG), Specific Growth Rate (SGR), Survival, Feed Conversion Ratio (FCR) and Protein Efficiency Ratio (PER) of milkfish juvenile. Polynomial regression analysis showed that the optimum dietary level of SBL to promote optimum growth in milkfish early juvenile is 3.56g/100g diet. Beyond this inclusion level, growth performance indices tend to decline. Higher carcass total protein and lipid contents were observed in treatment groups fed diets with optimum inclusion levels of SBL compared to the other treatments. Collectively the findings suggest that incorporating SBL at an optimum dose of 3.56g/100g diet enhances survival, overall growth performance, and improves carcass composition of early juvenile milkfish. Dietary SBL inclusion could be a practical approach in improving the efficiency of juvenile milkfish nursery production to meet the requirements of the expanding cage aquaculture production systems