Expression patterns of Passiflora edulis APETALA1/FRUITFULL homologues shed light onto tendril and corona identities

Abstract Background Passiflora (passionflowers) makes an excellent model for studying plant evolutionary development. They are mostly perennial climbers that display axillary tendrils, which are believed to be modifications of the inflorescence. Passionflowers are also recognized by their unique flower features, such as the extra whorls of floral organs composed of corona filaments and membranes enclosing the nectary. Although some work on Passiflora organ ontogeny has been done, the developmental identity of both Passiflora tendrils and the corona is still controversial. Here, we combined ultrastructural analysis and expression patterns of the flower meristem and floral organ identity genes of the MADS-box AP1/FUL clade to reveal a possible role for these genes in the generation of evolutionary novelties in Passiflora. Results We followed the development of structures arising from the axillary meristem from juvenile to adult phase in P. edulis. We further assessed the expression pattern of P. edulis AP1/FUL homologues (PeAP1 and PeFUL), by RT-qPCR and in situ hybridization in several tissues, correlating it with the developmental stages of P. edulis. PeAP1 is expressed only in the reproductive stage, and it is highly expressed in tendrils and in flower meristems from the onset of their development. PeAP1 is also expressed in sepals, petals and in corona filaments, suggesting a novel role for PeAP1 in floral organ diversification. PeFUL presented a broad expression pattern in both vegetative and reproductive tissues, and it is also expressed in fruits. Conclusions Our results provide new molecular insights into the morphological diversity in the genus Passiflora. Here, we bring new evidence that tendrils are part of the Passiflora inflorescence. This points to the convergence of similar developmental processes involving the recruitment of genes related to flower identity in the origin of tendrils in different plant families. The data obtained also support the hypothesis that the corona filaments are likely sui generis floral organs. Additionally, we provide an indication that PeFUL acts as a coordinator of passionfruit development

A Possible Role Of The Thalamus In Some Cases Of Sudden Unexpected Death In Epilepsy [9]

[No abstract available]48510361037Betting, L.E., Mory, S.B., Li, L.M., Lopes-Cendes, I., Guerreiro, M.M., Guerreiro, C.A., Cendes, F., Voxel-based morphometry in patients with idiopathic generalized epilepsies (2006) Neuroimage, 32, pp. 498-502Betting, L.E., Mory, S.B., Lopes-Cendes, I., Li, L.M., Guerreiro, M.M., Guerreiro, C.A., Cendes, F., MRI volumetry shows increased anterior thalamic volumes in patients with absence seizures (2006) Epilepsy Behav, 8, pp. 575-580Bonilha, L., Rorden, C., Castellano, G., Cendes, F., Li, L.M., Voxel-based morphometry of the thalamus in patients with refractory medial temporal lobe epilepsy (2005) Neuroimage, 25, pp. 1016-1021Boyko, W.J., Galabru, C.K., McGeer, E.G., McGeer, P.L., Thalamic injections of kainic acid produce myocardial necrosis (1979) Life Sci, 25, pp. 87-98Norden, A.D., Blumenfeld, H., The role of subcortical structures in human epilepsy (2002) Epilepsy Behav, 3, pp. 219-231Scorza, F.A., Sanabria, E.R.G., Calderazzo, L., Cavalheiro, E.A., Glucose utilization during interictal intervals in an epilepsy model induced by pilocarpine: A qualitative study (1998) Epilepsia, 39, pp. 1041-1045Scorza, F.A., Arida, R.M., Priel, M., Calderazzo, L., Cavalheiro, E.A., The contribution of the lateral posterior and anteroventral thalamic nuclei on spontaneous recurrent seizures in the pilocarpine model of epilepsy (2002) Arq Neuropsiquiatr, 60, pp. 572-575Tomson, T., Walczak, T., Sillanpaa, M., Sander, J.W., Sudden unexpected death in epilepsy: A review of incidence and risk factors (2005) Epilepsia, 46, pp. 54-6

Sudden Unexpected Death In Epilepsy: Small Rnas Raise Expectations

[No abstract available]293591593Sander, J.W., The epidemiology of epilepsy revisited (2003) Curr Opin Neurol, 16, pp. 165-170de Boer, H.M., Mula, M., Sander, J.W., The global burden and stigma of epilepsy (2008) Epilepsy Behav, 12, pp. 540-546Nashef, L., Ryvlin, P., Sudden unexpected death in epilepsy (SUDEP): update and reflections (2009) Neurol Clin, 27, pp. 1063-1074Hesdorffer, D.C., Tomson, T., Benn, E., Sander, J.W., Nilsson, L., Langan, Y., Combined analysis of risk factors for SUDEP (2011) Epilepsia, 52, pp. 1150-1159Nei, M., Hays, R., Sudden unexpected death in epilepsy (2010) Curr Neurol Neurosci Rep, 10, pp. 319-326Nashef, L., Sudden unexpected death in epilepsy: terminology and definitions (1997) Epilepsia, 38, pp. S6-S8Ficker, D.M., So, E.L., Shen, W.K., Annegers, J.F., O'Brien, P.C., Cascino, G.D., Population-based study of the incidence of sudden unexplained death in epilepsy (1998) Neurology, 51, pp. 1270-1274Schuele, S.U., Widdess-Walsh, P., Bermeo, A., Lüders, H.O., Sudden unexplained death in epilepsy: the role of the heart (2007) Cleve Clin J Med, 74, pp. S121-S127Surges, R., Thijs, R.D., Tan, H.L., Sander, J.W., Sudden unexpected death in epilepsy: risk factors and potential pathomechanisms (2009) Nat Rev Neurol, 5, pp. 492-504Tomson, T., Nashef, L., Ryvlin, P., Sudden unexpected death in epilepsy: current knowledge and future directions (2008) Lancet Neurol, 7, pp. 1021-1031Stollberger, C., Finsterer, J., Cardiorespiratory findings in sudden unexplained/unexpected death in epilepsy (SUDEP) (2004) Epilepsy Res, 59, pp. 51-60Surges, R., Taggart, P., Sander, J.W., Walker, M.C., Too long or too short? New insights into abnormal cardiac repolarization in people with chronic epilepsy and its potential role in sudden unexpected death (2010) Epilepsia, 51, pp. 738-744Nei, M., Ho, R.T., Abou-Khalil, B.W., Drislane, F.W., Liporace, J., Romeo, A., EEG and ECG in sudden unexplained death in epilepsy (2004) Epilepsia, 45, pp. 338-345Nashef, L., Hindocha, L., MakoffRisk, A., Factors in sudden death in epilepsy (SUDEP): the quest for mechanisms (2007) Epilepsia, 48, pp. 859-871Reis, L.O., Pereira, T.C., Lopes-Cendes, I., Ferreira, U., MicroRNAs: a new paradigm on molecular urological oncology (2010) Urology, 76, pp. 521-527Xu, J., Zhao, J., Evan, G., Xiao, C., Cheng, Y., Xiao, J., Circulating microRNAs: novel biomarkers for cardiovascular diseases (2012) J Mol Med (Berl), 90 (8), pp. 865-875Chen, C., Yang, S., Wang, F., Long, G., Yang, X., Chen, F., Plasma microRNA-361-5p as a biomarker of chronic heart failure (2010) Heart, 96, pp. A189Dogini, D.B., Ribeiro, P.A., Rocha, C., Pereira, T.C., Lopes-Cendes, I., MicroRNA expression profile in murine central nervous system development (2008) J Mol Neurosci, 35, pp. 331-337Vo, N.K., Cambronne, X.A., Goodman, R.H., MicroRNA pathways in neural development and plasticity (2010) Curr Opin Neurobiol, 20, pp. 457-465Ma, R., Sana, J., Faltejskova, P., Svoboda, M., Slaby, O., Novel classes of non-coding RNAs and cancer (2012) J Transl Med, 10, p. 103Cheng, Y., Tan, N., Yang, J., Liu, X., Cao, X., He, P., A translational study of circulating cell-free microRNA-1 in acute myocardial infarction (2010) Clin Sci (Lond), 119, pp. 87-95Ji, X., Takahashi, R., Hiura, Y., Hirokawa, G., Fukushima, Y., Iwai, N., Plasma miR-208 as a biomarker of myocardial injury (2009) Clin Chem, 55, pp. 1944-1949Fichtlscherer, S., De Rosa, S., Fox, H., Schwietz, T., Fischer, A., Liebetrau, C., Circulating microRNAs in patients with coronary artery disease (2010) Circ Res, 107, pp. 677-684De Rosa, S., Fichtlscherer, S., Lehmann, R., Assmus, B., Dimmeler, S., Zeiher, A.M., Transcoronary concentration gradients of circulating microRNAs (2011) Circulation, 124, pp. 1936-1944Surges, R., Sander, J.W., Sudden unexpected death in epilepsy: mechanisms, prevalence, and prevention (2012) Curr Opin Neurol, 25, pp. 201-20