Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection

Background Insect haemocytes mediate cellular immune responses (e.g., phagocytosis) and contribute to the synthesis of humoral immune factors. In previous work, a genome-wide molecular characterization of Anopheles gambiae circulating haemocytes was followed by functional gene characterization using cell-based RNAi screens. Assays were carried out to investigate the role of selected haemocyte-specific or enriched genes in phagocytosis of bacterial bio-particles, expression of the antimicrobial peptide cecropin1, and basal and induced expression of the mosquito complement factor LRIM1 (leucine-rich repeat immune gene I). Findings Here we studied the impact of a subset of genes (37 candidates) from the haemocyte-specific dsRNA collection on the development of Plasmodium in the mosquito by in vivo gene silencing. Our screening identifies 10 novel factors with a role in the mosquito response to Plasmodium. Analysis of in vivo screening phenotypes reveals a significant anti-correlation between the prevalence of oocysts and melanised ookinetes. Conclusions Among novel immune genes are putative pattern recognition proteins (leucine-rich repeat, fibrinogen-domain and R-type lectins), immune modulation and signalling proteins (LPS-induced tumor necrosis factor alpha factor, LITAF and CLIP proteases), and components of extracellular matrix such as laminin and collagen. Additional identified proteins such as the storage protein hexamerin and vesicular-type ATPase (V-ATPase) are associated for the first time with the mosquito response against Plasmodium

Decoherence in a Two Slit Diffraction Experiment with Massive Particles

Matter-wave interferometry has been largely studied in the last few years. Usually, the main problem in the analysis of the diffraction experiments is to establish the causes for the loss of coherence observed in the interference pattern. In this work, we use different type of environmental couplings to model a two slit diffraction experiment with massive particles. For each model, we study the effects of decoherence on the interference pattern and define a visibility function that measures the loss of contrast of the interference fringes on a distant screen. Finally, we apply our results to the experimental reported data on massive particles $C_{70}$.Comment: 6 pages, 3 figure

Onset of classical behaviour after a phase transition

We analyze the onset of classical behaviour in a scalar field after a continuous phase transition, in which the system-field, the long wavelength order parameter of the model, interacts with an environment of its own short-wavelength modes. We compute the decoherence time for the system-field modes from the master equation and compare it with the other time scales of the model. Within our approximations the decoherence time is in general the smallest dynamical time scale. Demanding diagonalisation of the decoherence functional produces identical results. The inclusion of other environmental fields makes diagonalisation occur even earlier.Comment: Seven pages, no figures. Contributed talk to the Second International Workshop DICE2004, Piombino, Italy. To be published in the Brazilian Journal of Physic

Model study of the sign problem in the mean-field approximation

We argue the sign problem of the fermion determinant at finite density. It is unavoidable not only in Monte-Carlo simulations on the lattice but in the mean-field approximation as well. A simple model deriving from Quantum Chromodynamics (QCD) in the double limit of large quark mass and large quark chemical potential exemplifies how the sign problem arises in the Polyakov loop dynamics at finite temperature and density. In the color SU(2) case our mean-field estimate is in excellent agreement with the lattice simulation. We combine the mean-field approximation with a simple phase reweighting technique to circumvent the complex action encountered in the color SU(3) case. We also investigate the mean-field free energy, from the saddle-point of which we can estimate the expectation value of the Polyakov loop.Comment: 14 page, 18 figures, typos corrected, references added, some clarification in sec.I

The effect of concurrent geometry and roughness in interacting surfaces

We study the interaction energy between two surfaces, one of them flat, the other describable as the composition of a small-amplitude corrugation and a slightly curved, smooth surface. The corrugation, represented by a spatially random variable, involves Fourier wavelengths shorter than the (local) curvature radii of the smooth component of the surface. After averaging the interaction energy over the corrugation distribution, we obtain an expression which only depends on the smooth component. We then approximate that functional by means of a derivative expansion, calculating explicitly the leading and next-to-leading order terms in that approximation scheme. We analyze the resulting interplay between shape and roughness corrections for some specific corrugation models in the cases of electrostatic and Casimir interactions.Comment: 14 pages, 3 figure

Vacuum fluctuations and generalized boundary conditions

We present a study of the static and dynamical Casimir effects for a quantum field theory satisfying generalized Robin boundary condition, of a kind that arises naturally within the context of quantum circuits. Since those conditions may also be relevant to measurements of the dynamical Casimir effect, we evaluate their role in the concrete example of a real scalar field in 1+1 dimensions, a system which has a well-known mechanical analogue involving a loaded string.Comment: 8 pages, 1 figur

Derivative expansion for the Casimir effect at zero and finite temperature in d+1d+1 dimensions

We apply the derivative expansion approach to the Casimir effect for a real scalar field in $d$ spatial dimensions, to calculate the next to leading order term in that expansion, namely, the first correction to the proximity force approximation. The field satisfies either Dirichlet or Neumann boundary conditions on two static mirrors, one of them flat and the other gently curved. We show that, for Dirichlet boundary conditions, the next to leading order term in the Casimir energy is of quadratic order in derivatives, regardless of the number of dimensions. Therefore it is local, and determined by a single coefficient. We show that the same holds true, if $d \neq 2$, for a field which satisfies Neumann conditions. When $d=2$, the next to leading order term becomes nonlocal in coordinate space, a manifestation of the existence of a gapless excitation (which do exist also for $d> 2$, but produce sub-leading terms). We also consider a derivative expansion approach including thermal fluctuations of the scalar field. We show that, for Dirichlet mirrors, the next to leading order term in the free energy is also local for any temperature $T$. Besides, it interpolates between the proper limits: when $T \to 0$ it tends to the one we had calculated for the Casimir energy in $d$ dimensions, while for $T \to \infty$ it corresponds to the one for a theory in $d-1$ dimensions, because of the expected dimensional reduction at high temperatures. For Neumann mirrors in $d=3$, we find a nonlocal next to leading order term for any $T>0$.Comment: 18 pages, 6 figures. Version to appear in Phys. Rev.

Using boundary methods to compute the Casimir energy

We discuss new approaches to compute numerically the Casimir interaction energy for waveguides of arbitrary section, based on the boundary methods traditionally used to compute eigenvalues of the 2D Helmholtz equation. These methods are combined with the Cauchy's theorem in order to perform the sum over modes. As an illustration, we describe a point-matching technique to compute the vacuum energy for waveguides containing media with different permittivities. We present explicit numerical evaluations for perfect conducting surfaces in the case of concentric corrugated cylinders and a circular cylinder inside an elliptic one.Comment: To be published in the Proceedings of QFEXT09, Norman, OK