Oviposition by European Corn Borer (Lepidoptera: Crambidae) in Response to Various Transgenic Corn Events

Oviposition preference by European corn borers, Ostrinia nubilalis (Hubner), for or against transgenic corn would influence amounts of refuge required for resistance management. The objective of this research was to determine if various Bacillus thuringiensis (Bt) corn transgenic events influence 0. nubilalis oviposition. All commercially available events (currently 5) were evaluated, plus 1 experimental event. Results from 3 independent studies are reported, including 3 field-cage experiments with vegetative corn, 2 field-cage experiments with reproductive corn, and 2 field experiments with natural 0. nubilalis on reproductive corn. In each case, Bt corn hybrids are compared with their near isogenic hybrids by counting numbers of egg masses on each plant type. More extensive comparisons were made in 3 of the experiments by determining the number, size, and location of egg masses on the corn hybrids. Moths laid more egg masses on Bt corn than on non-Bt corn in 1 cage experiment. These results, however, were not found in any of the other experiments. There is evidence that suggests cage effects influence moth oviposition more than Bt protein. Four of the 5 cage experiments and 2 field experiments indicate that the tested Bt events do not influence 0. nubilalis oviposition. Larval injury to isogenic corn during the vegetative stage did not influence adult oviposition during the corn reproductive stage when compared with Bt corn and noninjured isogenic corn. Based on these experiments, suggestions are made for future studies that use natural 0. nubilalis rather than 0. nubilalis in cages

Climate change impacts on mycotoxin risks in US maize

To ensure future food security, it is crucial to understand how potential climate change scenarios will affect agriculture. One key area of interest is how climatic factors, both in the near- and the long-term future, could affect fungal infection of crops and mycotoxin production by these fungi. The objective of this paper is to review the potential impact of climate change on three important mycotoxins that contaminate maize in the United States, and to highlight key research questions and approaches for understanding this impact. Recent climate change analyses that pertain to agriculture and in particular to mycotoxigenic fungi are discussed, with respect to the climatic factors – temperature and relative humidity – at which they thrive and cause severe damage. Additionally, we discuss how climate change will likely alter the life cycles and geographic distribution of insects that are known to facilitate fungal infection of crops

Genetics, sleep and memory:a recall-by-genotype study of ZNF804A variants and sleep neurophysiology

© 2015 Hellmich et al.Background: Schizophrenia is a complex, polygenic disorder for which over 100 genetic variants have been identified that correlate with diagnosis. However, the biological mechanisms underpinning the different symptom clusters remain undefined. The rs1344706 single nucleotide polymorphism within ZNF804A was among the first genetic variants found to be associated with schizophrenia. Previously, neuroimaging and cognitive studies have revealed several associations between rs1344706 and brain structure and function. The aim of this study is to use a recall-by-genotype (RBG) design to investigate the biological basis for the association of ZNF804A variants with schizophrenia. A RBG study, implemented in a population cohort, will be used to evaluate the impact of genetic variation at rs1344706 on sleep neurophysiology and procedural memory consolidation in healthy participants. Methods/Design: Participants will be recruited from the Avon Longitudinal Study of Parents and Children (ALSPAC) on the basis of genotype at rs1344706 (n = 24). Each participant will be asked to take part in two nights of in-depth sleep monitoring (polysomnography) allowing collection of neurophysiological sleep data in a manner not amenable to large-scale study. Sleep questionnaires will be used to assess general sleep quality and subjective sleep experience after each in-house recording. A motor sequencing task (MST) will be performed before and after the second night of polysomnography. In order to gather additional data about habitual sleep behaviour participants will be asked to wear a wrist worn activity monitor (actiwatch) and complete a sleep diary for two weeks. Discussion: This study will explore the biological function of ZNF804A genotype (rs1344706) in healthy volunteers by examining detailed features of sleep architecture and physiology in relation to motor learning. Using a RBG approach will enable us to collect precise and detailed phenotypic data whilst achieving an informative biological gradient. It would not be feasible to collect such data in the large sample sizes that would be required under a random sampling scheme. By dissecting the role of individual variants associated with schizophrenia in this way, we can begin to unravel the complex genetic mechanisms of psychiatric disorders and pave the way for future development of novel therapeutic approaches

History of discovery of the patagonian lizards

Knowledge of the history is necessary to understand why things are today as they are. Argentinean and Chilean Patagonia have a very interesting story about the native fauna and its discovery. The main character of this story is an adventurer spirit wanting to increase knowledge by traveling to the “end of the world”, ignoring barriers only to search and see what is beyond. Many well-known naturalists have visited this land eager and willing to find new species never seen before, while others have made some amazing contributions while never setting one foot on Patagonian soil. In this chapter, we intend to summarize how Patagonian herpetofauna was discovered, described and studied over time. In addition, we want to mention important scientists, whose work led the way for the future researchers to come.Fil: Williams, Jorge Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Zoología de Vertebrados. Sección Herpetología; ArgentinaFil: Kass, Camila Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Zoología de Vertebrados. Sección Herpetología; ArgentinaFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentin

Convex Shape and Rotation Model of Lucy Target (11351) Leucus from Lightcurves and Occultations

We report new photometric lightcurve observations of the Lucy Mission target (11351) Leucus acquired during the 2017, 2018, and 2019 apparitions. We use these data in combination with stellar occultations captured during five epochs to determine the sidereal rotation period, the spin axis orientation, a convex shape model, the absolute scale of the object, its geometric albedo, and a model of the photometric properties of the target. We find that Leucus is a prograde rotator with a spin axis located within a sky-projected radius of 3° (1σ) from J2000 Ecliptic coordinates (λ = 208°, β = +77°) or J2000 Equatorial Coordinates (R.A. = 248°, decl. = +58°). The sidereal period is refined to P_(sid) = 445.683 ± 0.007 h. The convex shape model is irregular, with maximum dimensions of 60.8, 39.1, and 27.8 km. The convex model accounts for global features of the occultation silhouettes, although minor deviations suggest that local and global concavities are present. We determine a geometric albedo of p_V = 0.043 ± 0.002. The derived phase curve supports a D-type classification for Leucus

Convex Shape and Rotation Model of Lucy Target (11351) Leucus from Lightcurves and Occultations

We report new photometric lightcurve observations of the Lucy Mission target (11351) Leucus acquired during the 2017, 2018 and 2019 apparitions. We use these data in combination with stellar occultations captured during five epochs (Buie et al. 2020) to determine the sidereal rotation period, the spin axis orientation, a convex shape model, the absolute scale of the object, its geometric albedo, and a model of the photometric properties of the target. We find that Leucus is a prograde rotator with a spin axis located within a sky-projected radius of 3{\deg} (1$\sigma$) from J2000 Ecliptic coordinates ($\lambda=208\deg$, $\beta=+77\deg$) or J2000 Equatorial Coordinates (RA=248$\deg$, Dec=+58$\deg$). The sidereal period is refined to $P_{sid}=445.683\pm0.007$ h. The convex shape model is irregular, with maximum dimensions of (60.8, 39.1, 27.8) km. The convex model accounts for global features of the occultation silhouettes, although minor deviations suggest that local and global concavities are present. We determine a geometric albedo $p_V=0.043\pm0.002$. The derived phase curve supports a D-type classification for Leucus

Improving Students’ Social Participation in Primary and Secondary Schools Across Europe

This handbook is the product of collaboration within the “FRIEND-SHIP—Improving Students’ Social Participation in Primary and Secondary Schools Across Europe” Project, funded by the European Commission—Erasmus+ Programme, Project No.: 2019-1-AT01-KA201-05 1226. One of the main outputs of this Project consisted of the development of the FRIEND-SHIP Program, a school-based intervention aimed at improving social participation of all students between 8 and 11 years of age. The FRIEND-SHIP Program is intended to be implemented by teachers and consists of 12 sessions, each with the duration of 45–60 minutes, implemented twice a week over six weeks. The activities that comprise this program are essentially experiential. Students are invited to take an active and involved role by participating in group activities, role-playing, social stories, arts, and physical activities. The main purpose of this handbook is to support teachers in implementing the FRIEND-SHIP program with their classes. This handbook is divided in three parts: • CHAPTERS 1, 2 and 3 cover background information about social participation and inclusion, as well as the description of the target audience of this handbook and the digital tool for evaluating the FRIEND-SHIP Program effects. • CHAPTER 4 covers the FRIEND-SHIP Intervention Program, including guiding principles, aims, and detailed descriptions of sessions. • CHAPTER 5 covers the information gathered with the implementation of the FRIEND-SHIP Intervention Program throughout the Project life.This handbook is the product of collaboration within the “FRIEND-SHIP—Improving Students’ Social Participation in Primary and Secondary Schools Across Europe” Project, funded by the European Commission—Erasmus+ Programme, Project No.: 2019-1-AT01-KA201-05 1226.info:eu-repo/semantics/publishedVersio

Schizophrenia-associated variation at <i>ZNF804A</i> correlates with altered experience-dependent dynamics of sleep slow waves and spindles in healthy young adults

The rs1344706 polymorphism in ZNF804A is robustly associated with schizophrenia and schizophrenia is, in turn, associated with abnormal non-rapid eye movement (NREM) sleep neurophysiology. To examine whether rs1344706 is associated with intermediate neurophysiological traits in the absence of disease, we assessed the relationship between genotype, sleep neurophysiology, and sleep-dependent memory consolidation in healthy participants. We recruited healthy adult males with no history of psychiatric disorder from the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort. Participants were homozygous for either the schizophrenia-associated 'A' allele (N = 22) or the alternative 'C' allele (N = 18) at rs1344706. Actigraphy, polysomnography (PSG) and a motor sequence task (MST) were used to characterize daily activity patterns, sleep neurophysiology and sleep-dependent memory consolidation. Average MST learning and sleep-dependent performance improvements were similar across genotype groups, albeit more variable in the AA group. During sleep after learning, CC participants showed increased slow-wave (SW) and spindle amplitudes, plus augmented coupling of SW activity across recording electrodes. SW and spindles in those with the AA genotype were insensitive to learning, whilst SW coherence decreased following MST training. Accordingly, NREM neurophysiology robustly predicted the degree of overnight motor memory consolidation in CC carriers, but not in AA carriers. We describe evidence that rs1344706 polymorphism in ZNF804A is associated with changes in the coordinated neural network activity that supports offline information processing during sleep in a healthy population. These findings highlight the utility of sleep neurophysiology in mapping the impacts of schizophrenia-associated common genetic variants on neural circuit oscillations and function

Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding

Macrophage infectivity potentiator (MIP) proteins are widespread in human pathogens including Legionella pneumophila, the causative agent of Legionnaires' disease and protozoans such as Trypanosoma cruzi. All MIP proteins contain a FKBP (FK506 binding protein)-like prolyl-cis/trans-isomerase domain that hence presents an attractive drug target. Some MIPs such as the Legionella pneumophila protein (LpMIP) have additional appendage domains of mostly unknown function. In full-length, homodimeric LpMIP, the N-terminal dimerization domain is linked to the FKBP-like domain via a long, free-standing stalk helix. Combining X-ray crystallography, NMR and EPR spectroscopy and SAXS, we elucidated the importance of the stalk helix for protein dynamics and inhibitor binding to the FKBP-like domain and bidirectional crosstalk between the different protein regions. The first comparison of a microbial MIP and a human FKBP in complex with the same synthetic inhibitor was made possible by high-resolution structures of LpMIP with a [4.3.1]-aza-bicyclic sulfonamide and provides a basis for designing pathogen-selective inhibitors. Through stereospecific methylation, the affinity of inhibitors to L. pneumophila and T. cruzi MIP was greatly improved. The resulting X-ray inhibitor-complex structures of LpMIP and TcMIP at 1.49 and 1.34 Å, respectively, provide a starting point for developing potent inhibitors against MIPs from multiple pathogenic microorganisms.</p