Frequencies of Lipopolysaccharide Core Types among Clinical Isolates of Escherichia coli Defined with Monoclonal Antibodies

Mouse monoclonal antibodies (MAbs) specific for the lipopolysaccharide (LPS) core types R1, R2, and R3 of Escherichia coli and a cross-reactive MAb that binds to the LPS core of almost all E. coli were used in ELISA to determine the frequency of cores resembling R1, R2, and R3 in strains of E. coli isolated from clinical samples (blood and urine specimens) and from the feces of asymptomatic individuals. Of the 180 wild-type isolates, 123 were assigned to R1 core type, 14 to R2, and 18 to R3. Twenty-five wild-type E. coli isolates could not be assigned to a particular core type and may have either an R4 or K12 core or a previously unrecognized core type. R1 core type was associated with O types 1, 4, 6, 8, and 18 and with K1 or K5 capsules. R3 was associated with O15.O75 isolates could be of either R1 or R2 core typ

Can the electronic nose diagnose cronic rhinosinusitis? A new experimental study

In otorhinolaryngologist's experience the nasal out-breath of people affected by chronic nasal or paranasal infections may be characterized by peculiar odours. In a previous study we showed that an electronic nose (EN), examining nasal out breath was able to distinguish subjects affected by chronic rhinosinusitis from healthy subjects. The present study is aimed at analysing the intensity and the quality of the odorous components present in the air expired by patients affected by rhinosinusitis, using a new EN based on gas-chromatography and surface acoustic wave analysis. In the gas-chromatographic tracings of the pathologic subjects there were six peaks, which were not present in control group cases. These peaks correspond to odorous components, whose chemical composition ranges from C6 to C14. Peaks obtained were compaired with other tracings revealed from specific bacterial and fungal cultures analyses and we appreciated some analogies

Che-1 activates XIAP expression in response to DNA damage

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