The role of extension gene in bovine coat color determination. Non-black phenotypes are associated with transcripts spliced in the 3 ' UTR region.

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Caracterization of different 5’ ultranslated exons of the ASIP gene in blak-and-tan Doberman Pinscher and brindle Boxer dogs

Differential expression of the ASIP gene and its interaction with MC1R have provided basic insight into pigment-type switching in mammals. Here, we report the characterization of a specific red-haired skin transcript and a specific black-haired skin transcript in the ASIP gene in the black-and-tan Doberman Pinscher. It is also shown that the brindle-haired skin of the Boxer exhibits a deregulated expression resulting in various 5'-untranslated exons. Comparative sequence analysis revealed a short interspersed element and a poly(A) stretch inserted within the promoter region of the ASIP in the Boxer. Genotyping studies have shown that both insertions are also present in brindle and fawn animals of the Boxer and Great Dane breeds. Furthermore, we genotyped MC1R and K loci for their known variants that affect coat color in dogs. As expected, all animals were homozygotes (E(M) /E(M) ) for the mask mutation, and fawn animals were k(y) /k(y) . Unexpectedly, we found that all brindle animals were heterozygotes k(B) /k(y) . Our results suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs

Characterization of different 5'-untranslated exons of the ASIP gene in black-and-tan Doberman Pinscher and brindle Boxer dogs.

Chantier qualité GAInternational audienceDifferential expression of the ASIP gene and its interaction with MC1R have provided basic insight into pigment-type switching in mammals. Here, we report the characterization of a specific red-haired skin transcript and a specific black-haired skin transcript in the ASIP gene in the black-and-tan Doberman Pinscher. It is also shown that the brindle-haired skin of the Boxer exhibits a deregulated expression resulting in various 5'-untranslated exons. Comparative sequence analysis revealed a short interspersed element and a poly(A) stretch inserted within the promoter region of the ASIP in the Boxer. Genotyping studies have shown that both insertions are also present in brindle and fawn animals of the Boxer and Great Dane breeds. Furthermore, we genotyped MC1R and K loci for their known variants that affect coat color in dogs. As expected, all animals were homozygotes (E(M) /E(M) ) for the mask mutation, and fawn animals were k(y) /k(y) . Unexpectedly, we found that all brindle animals were heterozygotes k(B) /k(y) . Our results suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs

Utilisation de marqueurs génétiques pour la traçabilité. Intérêt des gènes de la coloration de la robe chez le bovin

Les marqueurs génétiques, qu’ils soient anonymes (microsatellites) ou responsables de phénotypes (gènes de la coloration, par exemple), constituent des outils efficaces pour identifier les individus ou les populations (races) et pour assurer leur traçabilité. On montre ici l’intérêt du gène extension pour le génotypage des bovins

Breed traceability of sheep meat using coat color genes: preliminary results.

The aim of this work was to verify the possibility to use molecular methodologies and optimize a deterministic method for the assessment of a genetic ovine meat using the analysis of coat color gene polymorphisms. The studied animals, 5 for each breed, belong to 7 Italian sheep breeds. This work has investigated the polymorphism of six candidate coat colour genes. Several polymorphisms were found and some of them are new. An Agouti allele that seems to be specific to the Altamurana Breed and an allele of TYRP2 specific to the Sarda Breed were identified. This research highlights the presence of characteristics that encourage further studies of these genes taking into consideration a larger number of animals

New insights on coat colour genetics in rabbit coming from candidate gene analysis - Nuove acquisizioni della genetica molecolare sul determinismo del colore del mantello nel coniglio

We recently showed that mutations in the rabbit MC1R gene are associated with coat colours in different breeds. Here, we completed the characterization of the MC1R gene and further supported the association of the 30 bp deletion in this gene with the production of pheomelanic coat colours. Moreover, we identified 4 single nucleotide polymorphisms in the agouti (agouti signaling protein, ASIP) gene. These markers will be useful in association studies with coat colour in different rabbit breed

A composite six bp in-frame deletion in the melanocortin 1 receptor (MC1R) gene is associated with the Japanese brindling coat colour in rabbits (Oryctolagus cuniculus)

In the domestic rabbit (Oryctolagus cuniculus), classical genetic studies have identified five alleles at the Extension locus: E D (dominant black), E S (steel, weaker version of E D ), E (wild type, normal extension of black), e J (Japanese brindling, mosaic distribution of black and yellow) and e (non-extension of black, yellow/red with white belly). Sequencing almost the complete coding sequence (CDS) of the rabbit MC1R gene, we recently identified two in-frame deletions associated with dominant black (c.280_285del6; alleles E D or E S ) and recessive red (c.304_333del30; allele e) coat colours. It remained to characterize the e J allele whose phenotypic effect is similar to the Orange and Sex-linked yellow loci of cat and Syrian hamster

Traçabilité des produits d'origine bovine au moyen de test moléculaires portant sur les gènes contrôlant la coloration de la robe

"Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-Access "absen