15 research outputs found
Calculation of the in-plane force constants and vibrational spectra of pyridine and its deuterated derivatives by the CNDO/2 force method
The effect of iron binding on the conformation of transferrin. A small angle x-ray scattering study.
Distance distribution functions, p(r), radii of gyration, Rg, and radii of gyration of cross section, Rq, of apotransferrin, monoferric transferrin, and diferric transferrin have been compared. The alteration of Rg and Rq upon iron binding has been determined by a difference method. An unusual feature of the stepwise structural changes of transferrin upon iron saturation is that binding of the first ferric ion is responsible for more than half of the whole change in Rq, whereas Rg alters significantly only after the binding of the second ferric ion
The effect of iron binding on the conformation of transferrin. A small angle x-ray scattering study
Quantitative analysis of the interaction between immune complex and Clq complement subcomponent. The role of interdomain interactions in rabbit IgG in binding of Clq to immune precipitates
Application of a Novel Small-Scale Sample Cleanup Procedure Prior to MALDI-TOF-MS for Rapid Pigment Fingerprinting of Red Wines
This study evaluates the anthocyanin and derived
pigment composition of
Vitis vinifera
red wines of Vranec,
Merlot, and Cabernet Sauvignon produced in 2006, 2007, and
2008 vintages from the Tikve
š
wine region in the Republic of
Macedonia.Their profile was established using matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF-MS) technique. A total of 22 anthocyanins
and derived pigments have been identified in the samples including 10 anthocyanins, 1 ethyl-bridged flavanol
–
anthocy-
anin adduct, and 11 pyranoanthocyanins. MALDI-TOF-MS
analysis was performed after solid-phase extraction of the
wines by using, for the first time, the Zip-Tip® C18 stationary
phase, introducing a novel small-scale sample cleanup proce-
dure prior to the rapid MALDI-TOF-MS fingerprinting of
wine samples. 2
′
,4
′
,6
′
-Trihydroxyacetophenone (dissolved in
acetonitrile/water 1:1,
v
/
v
) was used as a matrix. The qualitative
screening of anthocyanins and derived pigments with MALDI-
TOF-MS confirmed the presence of glucoside, acetylglucoside,
and
p
-coumaroylglucoside derivatives of anthocyanins in the
wine samples. Furthermore, pyranoanthocyanins formed by
reactions of anthocyanins with pyruvic acid and acetaldehyde,
as well as flavanol
–
pyranoanthocyanins and ethyl-bridged
flavan-3-ol-anthocyanin adduct pigments have been detected
in the samples