On the presence, distribution and conservation status of Lycopodium lagopus (Lycopodiaceae) in Italy

During our work on the Lycopodiaceae account for the upcoming Flora Critica d\u2019Italia (1, 2), we assessed and accepted the presence in Italy of Lycopodium lagopus (Laest. ex Hartm.) Zinserl. ex Kuzen. = L. clavatum subsp. monostachyon (Grev. & Hook.) Selander (2). Already reported by Fiori (3) as L. clavatum f. monostachyum Desv., its presence in Italy was more recently confirmed by Tribsch & Sch\uf6nswetter (4) and accepted in some subsequent regional works (e.g. 5, 6), but the taxon is not recognized as distinct in the last national checklist of vascular plants (7). Lycopodium lagopus has an arctic-alpine distribution in America and Eurasia (8, 9). Initially described as a variety of L. clavatum L., the taxon was later raised to subspecific (e.g. 10, 11) and specific rank (8, 9). In view of its largely sympatric occurrence with L. clavatum in the Alps, and of the apparent absence of intermediate populations or individuals, we prefer to treat L. lagopus as a separate species. The main characters distinguishing L. lagopus from L. clavatum are the number of strobili (usually 1, rarely 2), and especially their being sessile or subsessile on a 0-2 cm long \u201cpeduncle\u201d. In the Italian Alps it usually occurs at >1800 m a.s.l. As a result of our revision of specimens in several Italian herbaria, this clubmoss, formerly known only from Trentino - Alto Adige and Lombardy, is here reported for the first time for Piedmont on the basis of two specimens collected by Carestia in Valsesia in 1870 and preserved in TO. The presence in Friuli - Venezia Giulia (reported in 6 on the basis of a posthumous work of Gortani) is not confirmed: a specimen collected by Gortani in 1908 and preserved in MFU under \u201cL. clavatum f. monostachyum Desv.\u201d is referable to L. clavatum. On the other hand, in view of the specie\u2019s ecology and confirmed distribution, one may reasonably expect that it is to be found, additionally, in Val d\u2019Aosta and Veneto. Lycopodium species in Europe have experienced a decline in abundance in a general way, partly due to their being collected and overexploited, and for this reason they have all been included in Annex V of the Habitats Directive 92/43/CEE. Lycopodium clavatum in particular, whose decline in Italy is confirmed by several authors (e.g., 6), is also included in Annex D of the Council Regulation (EC) No 338/97 on the protection of species of wild fauna and flora by regulation their trade. It is therefore urgent to define the current distribution and conservation status of L. lagopus in Italy, in order to plan possible conservation measures. We hope that its inclusion (as L. clavatum subsp. monostachyum) in the most recent Red List of Italian Flora (12), even if only as DD (Data Deficient), and the contribution here presented may stimulate the study of this species in Italy. This study is part of the \u201cFlora Critica d\u2019Italia\u201d project and as such was funded by the Societ\ue0 Botanica Italiana onlus, the Fondazione per la Flora Italiana, and the International Foundation Pro Herbario Mediterraneo. 1) L. Pignotti (ed) (2006) Progetto per una Flora critica dell\u2019Italia. Societ\ue0 Botanica Italiana, Firenze 2) A. Troia, W. Greuter (2013) Proceedings of XIV OPTIMA Meeting, Palermo, 9-15 September 2013, p. 151 3) A. Fiori (1943) Flora Italica Cryptogama, pars V: Pteridophyta. Tipografia Mariano Ricci, Firenze 4) A. Tribsch, Sch\uf6nswetter P. (1999) Verh. Zool.-Bot. Ges. 6sterreich, 136, 235-248 5) D. Aeschimann, K. Lauber, D.M. Moser, J.P. Theurillat (2004) Flora Alpina. Haupt Verlag, Bern 6) E. Bona (ed), F. Martini, H. Niklfeld, F. Prosser (2005) Atlante corologico delle Pteridofite nell\u2019Italia nordorientale. Distribution Atlas of the Pteridophytes of North-Eastern Italy. Museo Civico di Rovereto, Edizioni Osiride 7) F. Conti, G. Abbate, A. Alessandrini, C. Blasi (eds) (2005) An annotated checklist of the Italian vascular flora. Palombi Editori, Roma 8) W.H. Wagner, J. Beitel (1993) Lycopodiaceae. In: Flora of North America Editorial Committee (eds) Flora of North America Vol. 2 Pteridophytes & Gymnosperms. Oxford University Press, New York, pp. 18-37 9) F.J. Rumsey (2007) Watsonia, 26, 477-480 10) W. Rothmaler, A.C. Jermy (1993) Lycopodium L. In: T.G. Tutin et al. (eds), Flora europaea vol. 1, ed. 2. Cambridge, p. 4 11) I. Kukkonen (2000) Lycopodiaceae. In: B. Jonsell (ed) Flora Nordica 1. The Bergius Foundation, Stockholm, pp. 1-13 12) G. Rossi et al. (2013) Lista Rossa della Flora Italiana. 1. Policy Species e altre specie minacciate. Comitato Italiano IUCN e Ministero dell\u2019Ambiente e delle Tutela del Territorio e del Mar

Toward Micro Wall-Climbing Robots Using Biomimetic Fibrillar Adhesives

Climbing is a challenging task for autonomous mobile robots primarily due to requirements for agile locomotion, and high maneuverability as well as robust and efficient attachment and detachment. A novel miniature wall-climbing robot is proposed. The robot is adapted for the wall-climbing task by taking advantage of down scaling and its low design. Challenges encountered during robot miniaturization and performances of the robot are reported. The miniature robot prototype proved to be able to climb on inclined surfaces with a slope of up to 90° at a speed of 3.3mm/s. It is equipped with sensors that enable it to avoid obstacles, follow walls and detect free-falls. It can be controlled by remote control or act autonomously. Animals, such as Geckos, have developed amazing climbing ability through micro- and nano-fibers on their feet. These structures have inspired the study of dry adhesion and the design of synthetic fibrillar pads presented in the paper

[18F]FDG Uptake in Adipose Tissue Is Not Related to Inflammation in Type 2 Diabetes Mellitus

PURPOSE: 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) uptake is a marker of metabolic activity and is therefore used to measure the inflammatory state of several tissues. This radionuclide marker is transported through the cell membrane via glucose transport proteins (GLUTs). The aim of this study is to investigate whether insulin resistance (IR) or inflammation plays a role in [18F]FDG uptake in adipose tissue (AT). PROCEDURES: This study consisted of an in vivo clinical part and an ex vivo mechanistic part. In the clinical part, [18F]FDG uptake in abdominal visceral AT (VAT) and subcutaneous AT (SAT) was determined using PET/CT imaging in 44 patients with early type 2 diabetes mellitus (T2DM) (age 63 [54-66] years, HbA1c [6.3 ± 0.4 %], HOMA-IR 5.1[3.1-8.5]). Plasma levels were measured with ELISA. In the mechanistic part, AT biopsies obtained from 8 patients were ex vivo incubated with [18F]FDG followed by autoradiography. Next, a qRT-PCR analysis was performed to determine GLUT and cytokine mRNA expression levels. Immunohistochemistry was performed to determine CD68+ macrophage infiltration and GLUT4 protein expression in AT. RESULTS: In vivo VAT [18F]FDG uptake in patients with T2DM was inversely correlated with HOMA-IR (r = - 0.32, p = 0.034), and positively related to adiponectin plasma levels (r = 0.43, p = 0.003). Ex vivo [18F]FDG uptake in VAT was not related to CD68+ macrophage infiltration, and IL-1ß and IL-6 mRNA expression levels. Ex vivo VAT [18F]FDG uptake was positively related to GLUT4 (r = 0.83, p = 0.042), inversely to GLUT3 (r = - 0.83, p = 0.042) and not related to GLUT1 mRNA expression levels. CONCLUSIONS: In vivo [18F]FDG uptake in VAT from patients with T2DM is positively correlated with adiponectin levels and inversely with IR. Ex vivo [18F]FDG uptake in AT is associated with GLUT4 expression but not with pro-inflammatory markers. The effect of IR should be taken into account when interpreting data of [18F]FDG uptake as a marker for AT inflammation

Taxonomic clarification in W-Mediterranean Androcymbium (Colchicaceae): A. wyssianum sunk in the synonymy of A.gramineum and A.europaeum restored

Capsule dehiscence has been used as a diagnostic character for W Mediterranean species of Androcymbium. Depending on the state of capsule maturity, the character, however, can be ambiguous in herbarium material. Based on morphological, phenological and cpDNA characters it is shown that misinterpretation of the capsule as indehiscent in the type material of A. gramineum has led to serious taxonomic confusion. The combined analyses produced evidence that A. gramineum of the population from the type locality at Essaouira, Morocco, is conspecific with A. wyssianum. A. gramineum is therefore the correct name for the species with dehiscent capsules, whereas the populations with indehiscent capsules at the Atlantic coast north of Essaouira and in SE Spain represent a second species, which is correctly named A. europaeu

A new approach to the assessment of lumen visibility of coronary artery stent at various heart rates using 64-slice MDCT

Coronary artery stent lumen visibility was assessed as a function of cardiac movement and temporal resolution with an automated objective method using an anthropomorphic moving heart phantom. Nine different coronary stents filled with contrast fluid and surrounded by fat were scanned using 64-slice multi-detector computed tomography (MDCT) at 50–100 beats/min with the moving heart phantom. Image quality was assessed by measuring in-stent CT attenuation and by a dedicated tool in the longitudinal and axial plane. Images were scored by CT attenuation and lumen visibility and compared with theoretical scoring to analyse the effect of multi-segment reconstruction (MSR). An average increase in CT attenuation of 144 ± 59 HU and average diminished lumen visibility of 29 ± 12% was observed at higher heart rates in both planes. A negative correlation between image quality and heart rate was non-significant for the majority of measurements (P > 0.06). No improvement of image quality was observed in using MSR. In conclusion, in-stent CT attenuation increases and lumen visibility decreases at increasing heart rate. Results obtained with the automated tool show similar behaviour compared with attenuation measurements. Cardiac movement during data acquisition causes approximately twice as much blurring compared with the influence of temporal resolution on image quality

Calcium scoring using 64-slice MDCT, dual source CT and EBT: a comparative phantom study

Purpose Assessment of calcium scoring (Ca-scoring) on a 64-slice multi-detector computed tomography (MDCT) scanner, a dual-source computed tomography (DSCT) scanner and an electron beam tomography (EBT) scanner with a moving cardiac phantom as a function of heart rate, slice thickness and calcium density. Methods and materials Three artificial arteries with inserted calcifications of different sizes and densities were scanned at rest (0 beats per minute) and at 50–110 beats per minute (bpm) with an interval of 10 bpm using 64-slice MDCT, DSCT and EBT. Images were reconstructed with a slice thickness of 0.6 and 3.0 mm. Agatston score, volume score and equivalent mass score were determined for each artery. A cardiac motion susceptibility (CMS) index was introduced to assess the susceptibility of Ca-scoring to heart rate. In addition, a difference (Δ) index was introduced to assess the difference of absolute Ca-scoring on MDCT and DSCT with EBT. Results Ca-score is relatively constant up to 60 bpm and starts to decrease or increase above 70 bpm, depending on scoring method, calcification density and slice thickness. EBT showed the least susceptibility to cardiac motion with the smallest average CMS-index (2.5). The average CMS-index of 64-slice MDCT (9.0) is approximately 2.5 times the average CMS-index of DSCT (3.6). The use of a smaller slice thickness decreases the CMS-index for both CT-modalities. The Δ-index for DSCT at 0.6 mm (53.2) is approximately 30% lower than the Δ-index for 64-slice MDCT at 0.6 mm (72.0). The Δ-indexes at 3.0 mm are approximately equal for both modalities (96.9 and 102.0 for 64-slice MDCT and DSCT respectively). Conclusion Ca-scoring is influenced by heart rate, slice thickness and modality used. Ca-scoring on DSCT is approximately 50% less susceptible to cardiac motion as 64-slice MDCT. DSCT offers a better approximation of absolute calcium score on EBT than 64-slice MDCT when using a smaller slice thickness. A smaller slice thickness reduces the susceptibility to cardiac motion and reduces the difference between CT-data and EBT-data. The best approximation of EBT on CT is found for DSCT with a slice thickness of 0.6 mm

Suprageneric nomenclature in the Caryophyllaceae

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149755/1/tax04760.pd