Daily Rhythms of Serum Vitamin D-Metabolites, Calcium and Phosphorus in Horses

Many physiological processes of domestic animals exhibit daily rhythmicity. The goal of the present study was to investigate the daily rhythms of calcium, inorganic phosphorus and 24,25-(OH)2-D3, 25-(OH)-D3 and 1,25-(OH)2-D3 in the blood serum of horses. Five Thoroughbred mares from the same farm, clinically healthy and placed in individual stalls, at the same environmental temperature and photoperiod were used. For 30 days prior to the study, the animals underwent the same pattern of daily activity. Blood samples were collected at 4 h-intervals for 48 consecutive h, starting at 08:00 h of the first day and finishing at 04:00 h of the second day, via intravenous cannula inserted into the jugular vein. Each individual sample was assessed for serum concentration of calcium and inorganic phosphorus by means of a UV spectrophotometric test, and serum concentration of 24, 25-(OH)-D3, 25-(OH)-D3, and 1,25-(OH)2-D3 were assessed by means of HPLC method. Data analysis was conducted by one-way repeated measures analysis of variance (ANOVA) and by the single cosinor method. ANOVA showed a significant effect of time on all the variables studied (p < 0.0001) and post-hoc test (SNK) showed significant differences (p < 0.001) comparing all the time intervals of 4 h, on either day. The application of the periodic model and the statistical analysis of the cosinor procedure allowed defining the periodic variables and their acrophases (expressed in hours) during the 2 days of monitoring. Calcium showed diurnal acrophases at 15:00 h for the 1st day and at 15:48 for the 2nd day; inorganic phosphorus showed diurnal coincident acrophases at 14:32 h both for the 1st and 2nd day and 25-(OH)-D3 showed diurnal acrophases at 14:08 h for the 1st day and at 15:04 h for the 2nd day. The results obtained could be useful for standardizing blood sampling according to the time of day and for optimizing the administration of these substances according to their circadian or other rhythms

Nitrogenous metabolism in Thoroughbred and Arabian foals from birth to five months of age

The maturation of nitrogen metabolism was studied in six Thoroughbred and six Arabian foals. Rectal temperature, heart and respiratory rates and serum concentration of several hematochemical parameters (total protein, urea, uric acid, creatinine, and albumine) were monitored from birth to 5 months of age. In both breeds, all the parameters except albumine showed significant differences over time (P<0.05). The two breeds did not differ from each other at any time point. Statistically significant decreases in urea and creatinine concentrations were related to the growth needs of foals during this critical period of development

BSM W W production with a jet veto

We consider the impact on W W production of the unique dimension-six operator coupling gluons to the Higgs field. In order to study this process, we have to appropriately model the effect of a veto on additional jets. This requires the resummation of large logarithms of the ratio of the maximum jet transverse momentum and the invariant mass of the W boson pair. We have performed such resummation at the appropriate accuracy for the Standard Model (SM) background and for a signal beyond the SM (BSM), and devised a simple method to interface jet-veto resummations with fixed-order event generators. This resulted in the fast numerical code MCFM-RE, the Resummation Edition of the fixed-order code MCFM. We compared our resummed predictions with parton-shower event generators and assessed the size of effects, such as limited detector acceptances, hadronisation and the underlying event, that were not included in our resummation. We have then used the code to compare the sensitivity of W W and Z Z production at the HL-LHC to the considered higher-dimension operator. We have found that W W can provide complementary sensitivity with respect to Z Z, provided one is able to control theory uncertainties at the percent-level. Our method is general and can be applied to the production of any colour singlet, both within and beyond the SM

The treatment of the infrared region in perturbative QCD

We discuss the contribution coming from the infrared region to NLO matrix elements and/or coefficient functions of hard QCD processes. Strictly speaking, this contribution is not known theoretically, since it is beyond perturbative QCD. For DGLAP evolution all the infrared contributions are collected in the phenomenological input parton distribution functions (PDFs), at some relatively low scale Q_0; functions which are obtained from a fit to the `global' data. However dimensional regularization sometimes produces a non-zero result coming from the infrared region. Instead of this conventional regularization treatment, we argue that the proper procedure is to first subtract from the NLO matrix element the contribution already generated at the same order in \alpha_s by the LO DGLAP splitting function convoluted with the LO matrix element. This prescription eliminates the logarithmic infrared divergence, giving a well-defined result which is consistent with the original idea that everything below Q_0 is collected in the PDF input. We quantify the difference between the proposed treatment and the conventional approach using low-mass Drell-Yan production and deep inelastic electron-proton scattering as examples; and discuss the potential impact on the `global' PDF analyses. We present arguments to show that the difference cannot be regarded as simply the use of an alternative factorization scheme.Comment: 15 pages, 5 figures, title changed, text considerably modified to improve presentation, and discussion section enlarge

Medical-grade silicone coated with rhamnolipid R89 is effective against Staphylococcus spp. Biofilms

Staphylococcus aureus and Staphylococcus epidermidis are considered two of the most important pathogens, and their biofilms frequently cause device-associated infections. Microbial biosurfactants recently emerged as a new generation of anti-adhesive and anti-biofilm agents for coating implantable devices to preserve biocompatibility. In this study, R89 biosurfactant (R89BS) was evaluated as an anti-biofilm coating on medical-grade silicone. R89BS is composed of homologues of the mono- (75%) and di-rhamnolipid (25%) families, as evidenced by mass spectrometry analysis. The antimicrobial activity against Staphylococcus spp. planktonic and sessile cells was evaluated by microdilution and metabolic activity assays. R89BS inhibited S. aureus and S. epidermidis growth with minimal inhibitory concentrations (MIC99) of 0.06 and 0.12 mg/mL, respectively and dispersed their pre-formed biofilms up to 93%. Silicone elastomeric discs (SEDs) coated by R89BS simple adsorption significantly counteracted Staphylococcus spp. biofilm formation, in terms of both built-up biomass (up to 60% inhibition at 72 h) and cell metabolic activity (up to 68% inhibition at 72 h). SEM analysis revealed significant inhibition of the amount of biofilm-covered surface. No cytotoxic effect on eukaryotic cells was detected at concentrations up to 0.2 mg/mL. R89BS-coated SEDs satisfy biocompatibility requirements for leaching products. Results indicate that rhamnolipid coatings are effective anti-biofilm treatments and represent a promising strategy for the prevention of infection associated with implantable devices

Production of scalar and pseudo-scalar Higgs bosons to next-to-next-to-leading order at hadron colliders

We consider the production of intermediate-mass CP-even and CP-odd Higgs bosons in proton-proton and proton-anti-proton collisions. We extend the recently published results for the complete next-to-next-to-leading order calculation for a scalar Higgs boson to the pseudo-scalar case and present details of the calculation that might be useful for similar future investigations. The result is based on an expansion in the limit of a heavy top quark mass and a subsequent matching to the expression obtained in the limit of infinite energy. For a Higgs boson mass of 120 GeV the deviation from the infinite-top quark mass result is small. For 300 GeV, however, the next-to-next-to-leading order corrections for a scalar Higgs boson exceed the effective-theory result by about 9% which increases to 22% in the pseudo-scalar case. Thus in this mass range the effect on the total cross section amounts to about 2% and 6%, respectively, which may be relevant in future precision studies.Comment: 29 page