The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.

The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed

Non-targeted insecticidal stress on the neotropical brown stink bug Euschistus heros

Lethal and sublethal insecticide effects on non-targeted pest species are frequently neglected but have potential consequences for pest management and secondary pest outbreaks. Here, the lethal and demographic effects of four soybean insecticides (chlorantraniliprole, deltamethrin, pyriproxyfen, and spinosad, which are used against caterpillars, whiteflies, and green stink bugs) on the brown stink bug Euschistus heros (F.), the main stink bug species currently attacking Neotropical soybean fields, were assessed. Deltamethrin exhibited drastic acute mortality in adult females of E. heros with a median lethal time (LT50) of 0.6 days, whereas pyriproxyfen treated females exhibited a survival similar to that of water-treated insects (LT50 of 89 and 67 days, respectively). Chlorantraniliprole and spinosad-treated females exhibited intermediate survival with a TL50 of 54 and 47 days, respectively. Further scrutiny of the three most selective insecticides (i.e., chlorantraniliprole, pyriproxyfen, and spinosad) using age-structured matrices to assess the demographic impact of these compounds under earlier exposure indicated that both pyriproxyfen and spinosad significantly reduced the population growth of the pest species by compromising the survival of eggs (50% reduction compared with water-treated insects) to a greater extent than pyriproxyfen and spinosad, but without impairing insect population growth as much. Therefore, although pyriproxyfen and spinosad, besides deltamethrin, are not used to target the Neotropical brown stink bug, these insecticides adversely affected this pest species at the dose recommended for application on the label, thereby preventing its outbreak under such conditions

Alterations on growth and cell organization of Giardia intestinalis trophozoites after treatment with KH-TFMDI, a novel class III histone deacetylase inhibitor

Giardia trophozoites have developed resistance mechanisms to currently available compounds, leading to treatment failures. In this context, the development of new additional agents is mandatory. Sirtuins, which are class III NAD+-dependent histone deacetylases, have been considered important targets for the development of new anti-parasitic drugs. Here, we evaluated the activity of KH-TFMDI, a novel 3-arylideneindolin-2-one-type sirtuin inhibitor, on G. intestinalis trophozoites. This compound decreased the trophozoite growth presenting an IC50 value lower than nicotinamide, a moderately active inhibitor of yeast and human sirtuins. Light and electron microscopy analysis showed the presence of multinucleated cell clusters suggesting that the cytokinesis could be compromised in treated trophozoites. Cell rounding, concomitantly with the folding of the ventro-lateral flange and flagella internalization, was also observed. These cells eventually died by a mechanism which lead to DNA/nuclear damage, formation of multi-lamellar bodies and annexin V binding on the parasite surface. Taken together, these data show that KH-TFMDI has significant effects against G. intestinalis trophozoites proliferation and structural organization and suggest that histone deacetylation pathway should be explored on this protozoon as target for chemotherapy

'Centenária ES8132': nova variedade clonal de café conilon de maturação tardia para o Espírito Santo.

A variedade "Centenária - ES8132" foi obtida do Programa de Melhoramento Genético do Incaper em parceria com a Embrapa Café e o apoio do Consórcio Pesquisa Café, com a utilização de diferentes estratégias de melhoramento e de experimentos conduzidos nas fazendas experimentais de Marilândia, Sooretama e Bananal do Norte, localizadas em ambientes representativos de cultivo do café conilon, das regiões noroeste, nordeste e sul do Espírito Santo, respectivamente.bitstream/item/214530/1/03-VARIEDADE-CAFE-CENTENARIA.pdf2. ed. rev

'Diamante ES8112': nova variedade clonal de café conilon de maturação precoce para o Espírito Santo.

A variedade "Diamante ES8112" foi obtida do Programa de Melhoramento Genético do Incaper em parceria com a Embrapa Café e o apoio do Consórcio Pesquisa Café, com a utilização de diferentes estratégias de melhoramento e de experimentos conduzidos nas fazendas experimentais de Marilândia, Sooretama e Bananal do Norte, localizadas em ambientes representativos de cultivo do café conilon, das regiões noroeste, nordeste e sul do Espírito Santo, respectivamente.bitstream/item/214519/1/01-VARIEDADE-CAFE-DIAMANTE.pdf2.ed. rev

'ES8122' - Jequitibá: nova variedade clonal de café conilon de maturação intermediária para o Espírito Santo.

A variedade "ES8122- Jequitibá" foi obtida do Programa de Melhoramento Genético do Incaper em parceria com a Embrapa Café e o apoio do Consórcio Pesquisa Café, com a utilização de diferentes estratégias de melhoramento e de experimentos conduzidos nas fazendas experimentais de Marilândia, Sooretama e Bananal do Norte, localizadas em ambientes representativos de cultivo do café conilon, das regiões noroeste, nordeste e sul do Espírito Santo, respectivamente.bitstream/item/214528/1/02-VARIEDADE-CAFE-JEQUITIBA.pdf2. ed. rev