136 research outputs found
Phenolic composition of hydrophilic extract of manna from sicilian Fraxinus angustifolia vahl and its reducing, antioxidant and anti-inflammatory activity in vitro
Manna, a very singular vegetable product derived from the spontaneous solidification of the sap of some Fraxinus species, has long been known for its mild laxative and emollient properties. In this work, a hydro-alcoholic extract of manna (HME) from Sicilian Fraxinus angustifolia Vahl was investigated using HPLC-DAD to find phenol components and using chemical and biological in vitro assays to determine its reducing, antioxidant and anti-inflammatory capacity. We identified elenolic acid, tyrosol, hydroxytyrosol, catechin, fraxetin, verbascoside, gallic acid, procyanidin-B1, and luteolin 3,7 glucoside, in order of abundance. Measurements of total antioxidant activity by Folin-Ciocalteu reaction and ferric reducing ability (FRAP), as well as of scavenger activity towards ABTS•+, DPPH•, and perferryl-myoglobin radicals, showed that the phytocomplex effectively reduced oxidants with different standard potentials. When compared with vitamin E, HME also behaved as an efficient chain-breaking antioxidant against lipoperoxyl radicals from methyl linoleate. In cellular models for oxidative stress, HME counteracted membrane lipid oxidation of human erythrocytes stimulated by tert-butyl hydroperoxide and prevented the generation of reactive oxygen species, as well as the GSH decay in IL-1β–activated intestinal normal-like cells. Moreover, in this in vitro intestinal bowel disease model, HME reduced the release of the pro-inflammatory cytokines IL-6 and IL-8. These findings may suggest that manna acts as an antioxidant and anti-inflammatory natural product in humans, beyond its well-known effects against constipation
Xenobiotic-metabolizing enzymes in the skin of rat, mouse, pig, guinea pig, man, and in human skin models
Mechanism of Brain Targeting by Dexibuprofen Prodrugs Modified with Ethanolamine-Related Structures
Ceramides as possible nutraceutical compounds : characterization of the ceramides of the moro blood orange (citrus sinensis)
Ceramides are presented as nutraceutical compounds for protection of colon carcinoma and as important
cosmetic preparation components, increasing absorption through the skin. Therefore, the ceramide (Cer) content of Moro blood
oranges was determined by mass spectrometry. A total of 114 Cer species were identified: 3c160 mg in the peels and 3c140 mg in
the pulp per kilogram of oranges, expressed as \u201cmilligram equivalents of d18:1,17:0 Cer\u201d. The predominant ceramides contained
4-hydroxy-8-sphingenine (t18:1\u3948) and 4-hydroxysphinganine (t18:0) as long-chain bases (LCBs) and fatty acids (FAs) with
different structures. In the pulp, t18:1\u3948- and t18:0-containing Cer species comprised 50.5 and 33.5% of the total, respectively,
11.5 and 3.5% non-hydroxylated FAs, respectively, 32.0 and 21.0% \u3b1-hydroxylated FAs, respectively, and 7.0 and 9.0% \u3b1,\u3b2-
hydroxylated FAs, respectively. In the peels, t18:1\u3948- and t18:0-containing species comprised 49.5 and 34.5% of the total,
respectively, 16.0 and 1.5% non-hydroxylated FAs, respectively, 31.5 and 29.0% \u3b1-hydroxylated FAs, respectively, and 2.0 and
4.0% \u3b1,\u3b2-hydroxylated FAs, respectively
Frazione triterpenoide di Olea europea var. oleaster (Hoffmanns. & Link) dell’aerale Sardo
Evaluation of in vitro percutaneous absorption of lorazepam and clonazepam from hydro-alcoholic gel formulations
Monoolein dispersions as delivery systems for indomethacin: in vitro and in vivo investigations
In vivo evaluation of topical protective effect of soybean-germ oil against UVB-induced cutaneous erythema
In-vitro and in-vivo evaluation of oligoethylene esters as dermal prodrugs of 18-b-glycirrethic acid.
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