75 research outputs found
Coma morphology and dust emission pattern of comet C/2020 F3 (NEOWISE)
The recent close approach of comet C/2020 F3 (NEOWISE) allowed us to study the morphology of its inner coma. From the measurement of the dust ejection velocity on spiral structures expanding around the nucleus, we estimated a mean deprojected expansion velocity Vd = 1.11 ± 0.08 km s-1. Assuming that a new shell formed after every rotation of the comet, a period of 7.8 ± 0.2 h was derived. The spin axis orientation was estimated at RA 210° ± 10°, Dec. +35° ± 10°. The coma morphology appears related to two strong, diametrically opposite emissions located at mid-latitudes on the nucleus. A qualitative modelling of the coma produced consistent results with a wide range of dust sizes (0.80-800 μm), with inversely correlated densities (0.003-3.0 g cm-3). Images taken with Vj and r-Sloan filters showed a greater concentration of dust in the first two shells, and an increasing density of radicals emitting in the B and V band passes from the third shell outwards. Striae-like structures in the tail suggest that dust particles have different sizes
Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa
In spermatozoa, the nuclear F-actin supports the acroplaxome, a subacrosomal structure involved in the correct exposure of several acrosomal membrane proteins; among them, the glycoprotein IZUMO1 is the major protein involved in sperm-oocyte fusion. Nuclear F-actin is also involved in sperm head shaping and chromosome compartmentalization. To date, few notions regarding the bivalent role of F-actin on sperm chromatin organization and IZUMO1 positioning have been reported. In our work, we characterized subcellular organization of F-actin in human high- and low-quality spermatozoa (A- and B-SPZ), respectively, showing that F-actin over-expression in sperm head of B-SPZ affected IZUMO1 localization. A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. Interestingly, F-actin depolymerization was also associated with a correct acrosome repositioning, thus to favor a proper acrosome reaction onset, with changes in sperm nuclear size parameters and histone acetylation rate reaching high-quality conditions. In conclusion, the current work shows a key role of F-actin in the control of IZUMO1 localization as well as chromatin remodeling and acetylation events
The small binary asteroid (939) Isberga
In understanding the composition and internal structure of asteroids, their
density is perhaps the most diagnostic quantity. We aim here to characterize
the surface composition, mutual orbit, size, mass, and density of the small
main-belt binary asteroid (939) Isberga. For that, we conduct a suite of
multi-technique observations, including optical lightcurves over many epochs,
near-infrared spectroscopy, and interferometry in the thermal infrared. We
develop a simple geometric model of binary systems to analyze the
interferometric data in combination with the results of the lightcurve
modeling. From spectroscopy, we classify Ibserga as a Sq-type asteroid,
consistent with the albedo of 0.14 (all uncertainties are
reported as 3- range) we determine (average albedo of S-types is 0.197
0.153, Pravec et al., 2012, Icarus 221, 365-387). Lightcurve analysis
reveals that the mutual orbit has a period of 26.6304 0.0001 h, is close
to circular, and has pole coordinates within 7 deg. of (225, +86) in ECJ2000,
implying a low obliquity of 1.5 deg. The combined analysis of lightcurves and
interferometric data allows us to determine the dimension of the system and we
find volume-equivalent diameters of 12.4 km and
3.6 km for Isberga and its satellite, circling each other on a
33 km wide orbit. Their density is assumed equal and found to be
g.cm, lower than that of the associated ordinary
chondrite meteorites, suggesting the presence of some macroporosity, but
typical of S-types of the same size range (Carry, 2012, P\&SS 73, 98-118). The
present study is the first direct measurement of the size of a small main-belt
binary. Although the interferometric observations of Isberga are at the edge of
MIDI capabilities, the method described here is applicable to others suites of
instruments (e.g, LBT, ALMA).Comment: 12 pages, 6 figures, 4 table
Asteroids' physical models from combined dense and sparse photometry and scaling of the YORP effect by the observed obliquity distribution
The larger number of models of asteroid shapes and their rotational states
derived by the lightcurve inversion give us better insight into both the nature
of individual objects and the whole asteroid population. With a larger
statistical sample we can study the physical properties of asteroid
populations, such as main-belt asteroids or individual asteroid families, in
more detail. Shape models can also be used in combination with other types of
observational data (IR, adaptive optics images, stellar occultations), e.g., to
determine sizes and thermal properties. We use all available photometric data
of asteroids to derive their physical models by the lightcurve inversion method
and compare the observed pole latitude distributions of all asteroids with
known convex shape models with the simulated pole latitude distributions. We
used classical dense photometric lightcurves from several sources and
sparse-in-time photometry from the U.S. Naval Observatory in Flagstaff,
Catalina Sky Survey, and La Palma surveys (IAU codes 689, 703, 950) in the
lightcurve inversion method to determine asteroid convex models and their
rotational states. We also extended a simple dynamical model for the spin
evolution of asteroids used in our previous paper. We present 119 new asteroid
models derived from combined dense and sparse-in-time photometry. We discuss
the reliability of asteroid shape models derived only from Catalina Sky Survey
data (IAU code 703) and present 20 such models. By using different values for a
scaling parameter cYORP (corresponds to the magnitude of the YORP momentum) in
the dynamical model for the spin evolution and by comparing synthetics and
observed pole-latitude distributions, we were able to constrain the typical
values of the cYORP parameter as between 0.05 and 0.6.Comment: Accepted for publication in A&A, January 15, 201
A multiresolution approach to automated classification of protein subcellular location images
<p>Abstract</p> <p>Background</p> <p>Fluorescence microscopy is widely used to determine the subcellular location of proteins. Efforts to determine location on a proteome-wide basis create a need for automated methods to analyze the resulting images. Over the past ten years, the feasibility of using machine learning methods to recognize all major subcellular location patterns has been convincingly demonstrated, using diverse feature sets and classifiers. On a well-studied data set of 2D HeLa single-cell images, the best performance to date, 91.5%, was obtained by including a set of multiresolution features. This demonstrates the value of multiresolution approaches to this important problem.</p> <p>Results</p> <p>We report here a novel approach for the classification of subcellular location patterns by classifying in multiresolution subspaces. Our system is able to work with any feature set and any classifier. It consists of multiresolution (MR) decomposition, followed by feature computation and classification in each MR subspace, yielding local decisions that are then combined into a global decision. With 26 texture features alone and a neural network classifier, we obtained an increase in accuracy on the 2D HeLa data set to 95.3%.</p> <p>Conclusion</p> <p>We demonstrate that the space-frequency localized information in the multiresolution subspaces adds significantly to the discriminative power of the system. Moreover, we show that a vastly reduced set of features is sufficient, consisting of our novel modified Haralick texture features. Our proposed system is general, allowing for any combinations of sets of features and any combination of classifiers.</p
Modeling of transfer phenomena on heterogeneous Ziegler catalysts. II. Experimental investigation of intraparticle mass transfer resistance during the polymerization of ethylene in slurry
Investigation of ethylene/butadiene copolymers microstructure by H-1 and C-13 NMR
International audienc
Effect of Temperature on the Stability of Plasmid pTG201 and Productivity of xylE Gene Product in Recombinant Escherichia coli: Development of a Two-stage Chemostat with Free and Immobilized Cells
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