Effect of fertilizer in controlling weeds under intercropping of pearl millet and red bean in Sistan region, Iran

This experiment was conducted at the Agriculture Research Center of Zabol University during 2007 cropping season. The experimental design was split plot, using randomized complete block design with three replications. The factors included were, main factors: unfertilized (F1), 100% fertilizer (F2), 100% manure (F3), 50% fertilizer + 50% manure (F4) and five sub factors: sole crop of millet (I1), 75% millet + 25% bean (I2), 50% millet + 50% bean (I3), 25% millet + 75% bean (I4) and sole crop of bean (I5). The plants were planted as replacement method. The results showed that the lowest dry matter for crops and total dry matter of weeds was achieved from 50% fertilizer + 50% manure treatment. Also, the highest total dry matter of millet and bean was obtained from this treatment. The highest dry matter of millet and bean was obtained from the sole crop of millet and bean. Further, the highest total dry matter of millet and bean was achieved from the intercropping treatments. The land equivalent ratio (LER) for most of the intercropping treatments was greater than one which indicated that intercropping had advantage over sole crop. Comparing the performance of sole crop and intercrop treatments, the results indicated that intercropping combinations were more advantageous in terms of weed control.Key words: Intercropping, pearl millet, red bean, weeds, land equivalent ratio, yield

Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage

The rad2 mutant of Schizosaccharomyces pombe is sensitive to UV irradiation and deficient in the repair of UV damage. In addition, it has a very high degree of chromosome loss and/or nondisjunction. We have cloned the rad2 gene and have shown it to be a member of the Saccharomyces cerevisiae RAD2/S. pombe rad13/human XPG family. Using degenerate PCR, we have cloned the human homolog of the rad2 gene. Human cDNA has 55% amino acid sequence identity to the rad2 gene and is able to complement the UV sensitivity of the rad2 null mutant. We have thus isolated a novel human gene which is likely to be involved both in controlling the fidelity of chromosome segregation and in the repair of UV-induced DNA damage. Its involvement in two fundamental processes for maintaining chromosomal integrity suggests that it is likely to be an important component of cancer avoidance mechanisms

Effect of irrigation frequency and application levels of sulphur fertilizer on water use efficiency and yield of Indian mustard (Brassica juncea)

A field experiment was conducted at Indian Agricultural Research Institute, New Delhi during the crop season of 2007 to 2008 and 2008 to 2009 to study the effect of irrigation and sulphur on yield and water use efficiency of Indian mustard (Brassica juncea var. PusaJagannath). The experiment was carried out in split plot design with three replications. The treatments consisted of three levels of irrigation in the main plots [no irrigation, one irrigation at 45 days after sowing (DAS), and two irrigations at 45 DAS and 90 DAS] and four levels of sulphur in sub-plots (0, 15, 30, and 45 kg S/ha). The results showed that in both years of experimentation, application of two irrigations significantly increased the India mustard yield as indicated by dry matter accumulation, seed production, biological/biomass yield, and harvest index, in comparison to no irrigation. Also, the application of two irrigations, been on par with one irrigation, significantly enhanced seed and biological yield to 20.6 and 78.0 q/ha respectively in the first year, and 22.9 and 86.7 q/ha respectively in the second year, and the highest harvest index was obtained from the application of two irrigations in both years of the study. Water use efficiency as indicated by seasonal consumptive water use increased progressively with the increasing number of irrigations. Also, the amount of soil moisture extraction by the crop from upper layers increased and those from lower depth decreased with increase in the level of irrigation (from no irrigation to two irrigations) in both years of the study. Application of two irrigations to mustard gave higher net (yield) return and benefit-cost (B: C ratio) over one irrigation, which in turn, gave higher net benefit-cost return and B: C ratio than no irrigation. The increasing levels of sulphur application increased Indian mustard dry matter accumulation, seed and biological yield and harvest index. Seasonal consumptive water use by the crop and water use efficiency increased progressively with the increase level of sulphur application up to 45 kg S/ha . Quantity of soil moisture extraction from deeper layer increased with increase in the levels of sulphur application.Key words: Indian mustard, Irrigation, Sulphur, Water use efficiency, Economic evaluation

Undulation Instability of Epithelial Tissues

Treating the epithelium as an incompressible fluid adjacent to a viscoelastic stroma, we find a novel hydrodynamic instability that leads to the formation of protrusions of the epithelium into the stroma. This instability is a candidate for epithelial fingering observed in vivo. It occurs for sufficiently large viscosity, cell-division rate and thickness of the dividing region in the epithelium. Our work provides physical insight into a potential mechanism by which interfaces between epithelia and stromas undulate, and potentially by which tissue dysplasia leads to cancerous invasion.Comment: 4 pages, 3 figure

A cyclic peptide inhibitor of HIF-1 heterodimerization that inhibits hypoxia signaling in cancer cells

Hypoxia inducible factor-1 (HIF-1) is a heterodimeric transcription factor that acts as the master regulator of cellular response to reduced oxygen levels, thus playing a key role in the adaptation, survival and progression of tumors. Here we report cyclo-CLLFVY, identified from a library of 3.2 million cyclic hexapeptides using a genetically encoded high-throughput screening platform, as an inhibitor of the HIF-1α/HIF-1β protein-protein interaction in vitro and in cells. The identified compound inhibits HIF-1 dimerization and transcription activity by binding to the PAS-B domain of HIF-1α, reducing HIF-1-mediated hypoxia response signaling in a variety of cell lines, without affecting the function of the closely related HIF-2 isoform. The reported cyclic peptide demonstrates the utility of our high-throughput screening platform for the identification of protein-protein interaction inhibitors, and forms the starting point for the development of HIF-1 targeted cancer therapeutics

The prevalence of depression and its association with self-management behaviors in chronic disease patients

Background: Co-morbid depression in patients with chronic disease is associated with weak self-care and high complications. Objective: The study aimed to determine the relationship of self-care behaviors and the kind of chronic disease with depression in adults with chronic disease in Isfahan, Iran. Methods: This cross-sectional study was conducted in government healthcare centers. A total of 483 eligible consent respondents participated from March 2015 to June 2015. The “Stanford self-management questionnaire” and the patient health questionnaire (PHQ-9) were used for the assessment of self-management behaviors and depression. Data were analyzed using the SPSS 17 software. Results: The prevalence of depression in participants was 14.7%. There was a significant relationship between self-management behaviors such as physical activity (P 0.001) and skeletal disease patients (OR = 1.70, 95% CI (1.03 - 2.77), P < 0.05) than in other chronic disease patients. Conclusions: We found a negative association between depression and self-care activities in Iranian patients with chronic diseases. Psychological treatment for depression should be considered to improve the prognosis of chronic diseases

Adenomyoepithelial tumours and myoepithelial carcinomas of the breast – a spectrum of monophasic and biphasic tumours dominated by immature myoepithelial cells

BACKGROUND: Adenomyoepithelial tumours and myoepithelial carcinomas of the breast are primarily defined by the presence of neoplastic cells with a myoepithelial immunophenotype. Current classification schemes are based on purely descriptive features and an assessment of individual prognosis is still problematic. METHODS: A series of 27 adenomyoepithelial tumours of the breast was analysed immunohistochemically with antibodies directed against various cytokeratins, p63, smooth muscle alpha-actin (SMA) and vimentin. Additionally, double immunofluorescence and comparative genomic hybridisation (CGH) was performed. RESULTS: Immunohistochemically, all the tumours showed a constant expression of high molecular weight cytokeratins (Ck) Ck5 and Ck14, p63, SMA and vimentin. With exception of one case diagnosed as myoepithelial carcinoma, all tested tumours expressed low molecular weight cytokeratin Ck18 in variable proportions of cells. Even in monophasic tumours lacking obvious glandular differentiation in conventional staining, a number of neoplastic cells still expressed those cytokeratins. Double immunofluorescence revealed tumour cells exclusively staining for Ck5/Ck14 in the presence of other cell populations that co-expressed high molecular weight Ck5/Ck14 as well as either low molecular weight Ck8/18 or SMA. Based on morphology, we assigned the series to three categories, benign, borderline and malignant. This classification was supported by a stepwise increase in cytogenetic alterations on CGH. CONCLUSION: Adenomyoepithelial tumours comprise a spectrum of neoplasms consisting of an admixture of glandular and myoepithelial differentiation patterns. As a key component SMA-positive cells co-expressing cytokeratins could be identified. Although categorisation of adenomyoepithelial tumours in benign, borderline and malignant was supported by results of CGH, any assessment of prognosis requires to be firmly based on morphological grounds. At present it is not yet clear, if and to what extent proposed Ck5-positive progenitor cells contribute to the immunohistochemical and morphological heterogeneity of these neoplasms of the breast

The relationship between sensory sensitivity and autistic traits in the general population.

Individuals with Autism Spectrum Disorders (ASDs) tend to have sensory processing difficulties (Baranek et al. in J Child Psychol Psychiatry 47:591–601, 2006). These difficulties include over- and under-responsiveness to sensory stimuli, and problems modulating sensory input (Ben-Sasson et al. in J Autism Dev Disorders 39:1–11, 2009). As those with ASD exist at the extreme end of a continuum of autistic traits that is also evident in the general population, we investigated the link between ASD and sensory sensitivity in the general population by administering two questionnaires online to 212 adult participants. Results showed a highly significant positive correlation (r = .775, p &#60; .001) between number of autistic traits and the frequency of sensory processing problems. These data suggest a strong link between sensory processing and autistic traits in the general population, which in turn potentially implicates sensory processing problems in social interaction difficulties

Immunocluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data

High-dimensional cytometry is an innovative tool for immune monitoring in health and disease, and it has provided novel insight into the underlying biology as well as biomarkers for a variety of diseases. However, the analysis of large multiparametric datasets usually requires specialist computational knowledge. Here, we describe ImmunoCluster (https://github.com/ kordastilab/ImmunoCluster), an R package for immune profiling cellular heterogeneity in highdimensional liquid and imaging mass cytometry, and flow cytometry data, designed to facilitate computational analysis by a nonspecialist. The analysis framework implemented within ImmunoCluster is readily scalable to millions of cells and provides a variety of visualization and analytical approaches, as well as a rich array of plotting tools that can be tailored to users’ needs. The protocol consists of three core computational stages: (1) data import and quality control; (2) dimensionality reduction and unsupervised clustering; and (3) annotation and differential testing, all contained within an R-based open-source framework