Identification of chemical composition and antibacterial properties Juniperus oxycedrus L. Subsp. oxycedrus leaf essential oil [Juniperus oxycedrus l. Subsp. oxycedrus yapraklarındaki uçucu yağın kimyasal yapısı ve antibakteriyel özelliklerinin belirlenmesi]

The constituents of leaf essential oil of Juniperus oxycedrus L. subsp. oxycedrus (JOO) from wild flora of Gevas-Van in Turkey (Mount Artos) were studied using Gas Chromatography-Mass Spectrometry (GC-MS) and their antibacterial activities were assessed. A total of 18 compounds representing 99.98% of leaves oil were identified. The oils of the plant are all dominated by monoterpenes. The main compounds of essential oil in leaves were Limonene (45.77%), ?-Pinene (23.94%), ß-Phellandrene (10.83%), ß-Pinene (5.68%), o-Cymene (3.30%), respectively. The essential oils were tested against Staphylacoccus aureus, Bacillus subtilis, Pseudomanas aeruginosa, Enterecoccus faecalis, Salmonella typhimurium, and Escherichia coli strains using the disc diffusion method. The diameter of the inhibition zones formed for bacteria were measured. The essential oils of JOO found to be active against all of the tested microorganisms and showed the susceptible inhibition zones. However, they were not as much as effective against bacterial strains when compared to ampicillin and ofloxacin. The extracts of JOO showed most significant antibacterial activity against Escherichia coli with inhibition zone diameter of 15 mm. The lowest inhibition zone diameter was Staphylococcus aureus with 11 mm. © 2018, Centenary University. All rights reserved

Antioxidant properties of Rosa pisiformis and its protective effect against isoproterenol-induced oxidative stress in rats [Rosa pisiformis’in antioksidant özellikleri ve izoproterenol ile ratlarda oluşturulan okdidatif strese karşı koruyucu etkisi]

Objective: In this study, Rosa pisiformis was evaluated for its antioxidant activity, vitamin (A, E, C), trace element (Fe, Cu, Zn, Mn, Cr, Se) and mineral (Ca, K, Mg, Na) levels, in addition to its cardioprotective effects on cardiac tissue antioxidant enzymes (GSH-Px, SOD, CAT) during isoproterenol (ISO) - induced oxidative stress. Methods: In the study, vitamin and mineral analysis was carried out using HPLC and ICP-MS method, R. P. antioxidant and antiradical properties were determined using spectrophotometer. Forty wistar albino rats were divided into four groups: control (0.9% NaCl), ISO (100 mg/kg), ISO (100 mg/kg) + R. P. (300 mg/kg) and R. P. (300 mg/ kg). ISO was injected subcutaneously into rats twice at an interval of 24 h for two consecutive days (on 28th and 29th day). R. P. fruit administered daily. The experimental period was continued for 30 days. Results: The results of the study indicated that R. P. fruit and leaves contain high levels of minerals and vitamins and possess antioxidant and antiradical activity and its fruits have positive effects on cardiac tissues. The results are supported with significant changes in antioxidant enzyme levels and histopathological examination. Conclusion: This study demonstrates that R. P. fruits in particular can be used to treat and prevent cardiac diseases. © 2016, Turkish Biochemistry Society. All rights reserved