132 research outputs found
Dual-species quantum degeneracy of potassium-40 and rubidium-87 on an atom chip
In this article we review our recent experiments with a 40K-87Rb mixture. We
demonstrate rapid sympathetic cooling of a 40K-87Rb mixture to dual quantum
degeneracy on an atom chip. We also provide details on efficient BEC
production, species-selective magnetic confinement, and progress toward
integration of an optical lattice with an atom chip. The efficiency of our
evaporation allows us to reach dual degeneracy after just 6 s of evaporation -
more rapidly than in conventional magnetic traps. When optimizing evaporative
cooling for efficient evaporation of 87Rb alone we achieve BEC after just 4 s
of evaporation and an 8 s total cycle time.Comment: 8 pages, 4 figures. To be published in the Proceedings of the 20th
International Conference on Atomic Physics, 2006 (Innsbruck, Austria
Dynamics of a tunable superfluid junction
We study the population dynamics of a Bose-Einstein condensate in a
double-well potential throughout the crossover from Josephson dynamics to
hydrodynamics. At barriers higher than the chemical potential, we observe slow
oscillations well described by a Josephson model. In the limit of low barriers,
the fundamental frequency agrees with a simple hydrodynamic model, but we also
observe a second, higher frequency. A full numerical simulation of the
Gross-Pitaevskii equation giving the frequencies and amplitudes of the observed
modes between these two limits is compared to the data and is used to
understand the origin of the higher mode. Implications for trapped matter-wave
interferometers are discussed.Comment: 8 pages, 7 figures; v3: Journal reference added, minor changes to
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Evidence against a Germ Plasm in the Milkweed Bug Oncopeltus fasciatus, a Hemimetabolous Insect
Primordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this "germ plasm" acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola) suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.Organismic and Evolutionary Biolog
Three-dimensional character of atom-chip-based rf-dressed potentials
We experimentally investigate the properties of radio-frequency-dressed
potentials for Bose-Einstein condensates on atom chips. The three-dimensional
potential forms a connected pair of parallel waveguides. We show that
rf-dressed potentials are robust against the effect of small magnetic-field
variations on the trap potential. Long-lived dipole oscillations of condensates
induced in the rf-dressed potentials can be tuned to a remarkably low damping
rate. We study a beam-splitter for Bose-Einstein condensates and show that a
propagating condensate can be dynamically split in two vertically separated
parts and guided along two paths. The effect of gravity on the potential can be
tuned and compensated for using a rf-field gradient.Comment: 9 pages, 7 figure
RF spectroscopy in a resonant RF-dressed trap
We study the spectroscopy of atoms dressed by a resonant radiofrequency (RF)
field inside an inhomogeneous magnetic field and confined in the resulting
adiabatic potential. The spectroscopic probe is a second, weak, RF field. The
observed line shape is related to the temperature of the trapped cloud. We
demonstrate evaporative cooling of the RF-dressed atoms by sweeping the
frequency of the second RF field around the Rabi frequency of the dressing
field.Comment: 7 figures, 8 pages; to appear in J. Phys.
Rapid sympathetic cooling to Fermi degeneracy on a chip
Neutral fermions present new opportunities for testing many-body condensed
matter systems, realizing precision atom interferometry, producing ultra-cold
molecules, and investigating fundamental forces. However, since their first
observation, quantum degenerate Fermi gases (DFGs) have continued to be
challenging to produce, and have been realized in only a handful of
laboratories. In this Letter, we report the production of a DFG using a simple
apparatus based on a microfabricated magnetic trap. Similar approaches applied
to Bose-Einstein Condensation (BEC) of 87Rb have accelerated evaporative
cooling and eliminated the need for multiple vacuum chambers. We demonstrate
sympathetic cooling for the first time in a microtrap, and cool 40K to Fermi
degeneracy in just six seconds -- faster than has been possible in conventional
magnetic traps. To understand our sympathetic cooling trajectory, we measure
the temperature dependence of the 40K-87Rb cross-section and observe its
Ramsauer-Townsend reduction.Comment: 5 pages, 4 figures (v3: new collision data, improved atom number
calibration, revised text, improved figures.
The genome of the crustacean Parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion
The amphipod crustacean Parhyale hawaiensis is a blossoming model system for studies of developmental mechanisms and more recently regeneration. We have sequenced the genome allowing annotation of all key signaling pathways, transcription factors, and non-coding RNAs that will enhance ongoing functional studies. Parhyale is a member of the Malacostraca clade, which includes crustacean food crop species. We analysed the immunity related genes of Parhyale as an important comparative system for these species, where immunity related aquaculture problems have increased as farming has intensified. We also find that Parhyale and other species within Multicrustacea contain the enzyme sets necessary to perform lignocellulose digestion ('wood eating'), suggesting this ability may predate the diversification of this lineage. Our data provide an essential resource for further development of Parhyale as an experimental model. The first malacostracan genome will underpin ongoing comparative work in food crop species and research investigating lignocellulose as an energy source
Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees
Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles became segregated. In previtellogenic follicles, Amvasa transcription was detected in the trophocytes, which appear to supply its mRNA to the growing oocyte. A similar picture was obtained for ovarioles of the stingless bee Melipona quadrifasciata, except that Amvasa expression was higher in the oocytes of previtellogenic follicles. The social bees differ in this respect from Drosophila, the model system for insect oogenesis, suggesting that changes in the sequence and expression pattern of vasa may have occurred during social evolution
The Phylogenetic Origin of oskar Coincided with the Origin of Maternally Provisioned Germ Plasm and Pole Cells at the Base of the Holometabola
The establishment of the germline is a critical, yet surprisingly evolutionarily
labile, event in the development of sexually reproducing animals. In the fly
Drosophila, germ cells acquire their fate early during
development through the inheritance of the germ plasm, a specialized maternal
cytoplasm localized at the posterior pole of the oocyte. The gene
oskar (osk) is both necessary and
sufficient for assembling this substance. Both maternal germ plasm and
oskar are evolutionary novelties within the insects, as the
germline is specified by zygotic induction in basally branching insects, and
osk has until now only been detected in dipterans. In order
to understand the origin of these evolutionary novelties, we used comparative
genomics, parental RNAi, and gene expression analyses in multiple insect
species. We have found that the origin of osk and its role in
specifying the germline coincided with the innovation of maternal germ plasm and
pole cells at the base of the holometabolous insects and that losses of
osk are correlated with changes in germline determination
strategies within the Holometabola. Our results indicate that the invention of
the novel gene osk was a key innovation that allowed the
transition from the ancestral late zygotic mode of germline induction to a
maternally controlled establishment of the germline found in many holometabolous
insect species. We propose that the ancestral role of osk was
to connect an upstream network ancestrally involved in mRNA localization and
translational control to a downstream regulatory network ancestrally involved in
executing the germ cell program
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