Analiza epidemiologiczna występowania chorób odkleszczowych w Samodzielnym Publicznym Zakładzie Opieki Zdrowotnej w Bielsku-Podlaskim

Introduction. Ticks can infect people with numerous pathogens causing various infectious (viral, bacterial) or invasive (parasitic, fungal) diseases.Aim. The main objective of the present work included the epidemiologic analysis of the occurrence of tick-borne diseases at the Independent Public Health Care Facility in Bielsk Podlaski.Material and Methods. An analysis of medical documentation stored in the hospital’s IT database of patients hospitalized in 2016, 2017 and 2018 was performed. Sixty-six patients admitted to the Observation and Infectious Disease Ward diagnosed with tick-borne diseases, were qualified for the study.Results. Analysis of the data showed that Lyme disease was diagnosed most often. It affected mainly middle-aged and elderly men. The second most-often diagnosed illness was TBM characterized by its seasonality. No other tick-transmitted diseases were recorded. Although not all patients were aware of having been bitten by a tick, their symptoms allowed the diagnosis of tick-related illnesses. After treatment relevant to each ailment all patients were discharged in a good state of health.Conclusions. The completed retrospective analysis of the documentation of hospitalized patients shows consistency with the work of other authors both with respect to the frequency of tick-borne disease diagnoses and the most often reported symptoms. It also confirms an upward trend with respect to tick-borne disease hospitalizations. (JNNN 2020;9(2):51–58)  Wstęp. Kleszcze mogą zarażać ludzi wieloma patogenami wywołującymi różne choroby zakaźne (wirusowe, bakteryjne) lub inwazyjne (pasożytnicze, grzybicze).Cel. Głównym celem niniejszej pracy była analiza epidemiologiczna występowania chorób odkleszczowych w Samodzielnym Publicznym Zakładzie Opieki Zdrowotnej w Bielsku Podlaskim.Materiał i metody. Dokonano analizy dokumentacji medycznej przechowywanej w informatycznej bazie danych szpitala w 2016, 2017 i 2018 r. Do badania zakwalifikowano sześćdziesięciu sześciu pacjentów przyjętych na Oddział Obserwacji i Chorób Zakaźnych, u których zdiagnozowano choroby odkleszczowe.Wyniki. Analiza danych wykazała, że najczęściej rozpoznawano boreliozę. Dotyczyło to głównie mężczyzn w średnim i starszym wieku. Drugą najczęściej diagnozowaną chorobą było kleszczowe zapalenie opon mózgowych, charakteryzujące się sezonowością. Nie odnotowano żadnych innych chorób przenoszonych przez kleszcze. Chociaż nie wszyscy pacjenci byli świadomi ukąszenia przez kleszcza, ich objawy pozwoliły na rozpoznanie chorób odkleszczowych. Po leczeniu związanym z każdą dolegliwością wszyscy chorzy zostali wypisani do domu w dobrym stanie zdrowia.Wnioski. Przeprowadzona retrospektywna analiza dokumentacji pacjentów hospitalizowanych wykazuje spójność z pracami innych autorów zarówno pod względem częstości rozpoznawania chorób odkleszczowych, jak i najczęściej zgłaszanych objawów. Potwierdza również tendencję wzrostową w zakresie hospitalizacji z powodu chorób przenoszonych przez kleszcze. (PNN 2020;9(2):51–58

Matrix Metalloproteinase 9 (MMP-9) and Interleukin-8 (IL-8) in Gingival Crevicular Fluid after Regenerative Therapy in Periodontal Intrabony Defects with and without Systemic Antibiotics-Randomized Clinical Trial.

The aim of our study was to assess changes in the levels of IL-8 and MMP-9 in gingival crevicular fluid (GCF) collected from the periodontal pocket before and after regenerative surgery with deproteinized bovine bone mineral (DBBM) and collagen membrane (GTR) either independently (DBBM/GTR) or with the postoperative administration of antibiotic (DBBM/GTR+AB). The study involved 41 patients, each with one intrabony defect. IL-8 and MMP-9 were determined before therapy and after 2 weeks, 4 weeks and 6 months following the surgical procedure by means of dedicated ELISA kits. No statistical differences were observed in the levels of IL-8 and MMP-9 after 2 weeks, 4 weeks and 6 months between the groups. The changes in the level of MMP-9 over time were not statistically significant in any group. The changes in the level of IL-8 were significant for the group given antibiotic but not in the nonantibiotic group in the follow-up period. IL-8 and MMP-9 were found to correlate positively but not after 4 weeks in the test group. Current assessment of IL-8 and MMP-9 obtained from GCF samples provides evidence that collagen matrix turnover occurs actively during the early healing phase in the periodontium after regenerative procedures. We observed positive correlations of MMP-9 and IL-8 throughout the study. However, we failed to reveal any differences regard parameters studied between the two groups

Clinical assessment of the efficacy of photodynamic therapy in the treatment of oral lichen planus

The study objective was clinical assessment of the efficacy of photodynamic therapy (PDT) in the treatment of oral lichen planus (OLP). There were 23 patients aged 31–82 included in the study with oral lichen planus diagnosed clinically and histopathologically. In all patients photodynamic therapy was performed with the use of chlorin e6 (Photolon(®)), containing 20 % chlorin e6 and 10 % dimethyl sulfoxide as a photosensitizer. PDT was performed using a semiconductor laser, with power up to 300 mW and a wavelength of 660 nm. A series of illumination sessions was conducted with the use of superficial light energy density of 90 J/cm(2). Changes of lesion size were monitored at one, two, five, and ten PDT appointments from the series of ten according to the authors' own method. The sizes of clinical OLP lesions exposed to PDT were reduced significantly (on average by 55 %). The best effects were observed for the lesions on the lining mucosa (57.6 %). The therapy was statistically significantly less effective when masticatory mucosa was affected (reduction, 30.0 %). Due to substantial efficacy and noninvasiveness, PDT can be useful in the treatment of OLP lesions

Spectrophotometric study of derivatives as potential brokers ksantonu UV

Streszczenie:Cel: Celem pracy było zbadanie właściwości promieniochronnych wybranych pochodnych kwasu cynamonowego. Materiał i metody: W badaniu wykorzystano cztery wybrane pochodne kwasu cynamonowego. Każda w swojej budowie zawierała ugrupowanie ksantonu i kwasu cynamonowego. 10mg każdej substancji rozcieńczono w 1 ml metanolu o gęstości 0,789 kg/dm3. Następnie każdą substancję rozcieńczano odpowiednio drugi i trzeci 1000-krotnie a pierwszy i czwarty 10000-krotnie. Do przeprowadzenia badania wykorzystano urządzenie spektrofotometr Cecil CE7200. Badanie zostało przeprowadzone w zakresie fali 210 – 400nm i prędkością 10nm/s. Wyniki: Pierwsza substancja wykazała dwa maksima absorpcji przy długości fali 218 nm i 303,5 nm, druga wykazała trzy 225,5 nm, 273,5 nm, 296 nm, trzecia również dwa przy długości fali 248,5 nm, 309,5 nm, natomiast czwarta posiadała dwa maksima 241 nm i 338,5 nm. Wnioski: Wybrane cztery pochodne kwasu cynamonowego wykazały działanie promieniochronne. Pierwszy i drugi związek posiadał widmo ochronne w zakresie promieniowania UVB, natomiast trzeci i czwarty również w niewielkim zakresie UVA. Największe właściwości absorpcyjne ale w zakresie promieniowania UVB wykazała substancja druga, co może być związane z obecnością ugrupowania cynamoilu oraz położenia podstawnika metoksylowego. Natomiast substancja trzecia zawierająca ugrupowanie piperazyny, może dodatkowo wykazywać właściwości antyoksydacyjne.Słowa kluczowe: filtry przeciwsłoneczne, pochodne kwasu cynamonowego, promieniowanie UV, ochrona przeciwsłoneczna.Abstract:Aims: The aim of this study was to investigate the properties of UV-selected derivatives of cinnamic acid.Material and Methods: In the study, there were used four selected cinnamic acid derivatives. Each structure contained xanthone and cinnamic acid moieties. 10 mg of each substance was diluted in 1 ml of methanol. Then diluted using serial dilutious methods to obtain 1000-bold (2,3) or 10 000-bold (1,4). Measurements were cerried on spektrofotometr Cecil CE7200 with length of 210 – 400 nm and 10 nm/s speed.Results: The first substance showed two maximum of absorption at 218 nm and 303.5 nm, the second at 225.5 nm, 273.5 nm, 296 nm, the third at 248.5 nm, 309.5 nm while fourth at 241 nm and 338.5 nm. Conclusions: The four selected derivatives of cinnamic acid showed an ultraviolet protection. All of the tested compounds revealed protective effect at UVB range. The third and the fourth compound also small protective effect in the UVA range. However, a third substance containing piperizine group, may also has antioxidant properties

Effect of systemic antibiotics on the outcomes of regenerative periodontal surgery in intrabony defects: a randomized, controlled, clinical study.

OBJECTIVES To assess the potential influence of systemic antibiotic administration on the healing of periodontal intrabony defects treated with deproteinized bovine bone mineral (DBBM) and collagen membrane. MATERIALS AND METHODS Forty-one intrabony defects were treated by means of DBBM and collagen membrane (GTR). Postoperatively, the patients received either systemic antibiotics (i.e., 1 g of amoxicillin, twice daily for 7 days) (test) or no antibiotics (control). Clinical attachment level (CAL), probing depth (PD), and gingival recession (GR) were measured at baseline and at 1 year following regenerative surgery. The depth of the intrabony component (INTRA DD) and its width (INTRA DW) were measured during surgery and after 1 year at reentry. The depth (RxD) and width (RxW) of the intrabony defects were evaluated radiographically at baseline and at 1 year. RESULTS No adverse events were observed in any of the two groups throughout the entire study period. In the test group, mean CAL changed from 8.7 ± 1.4 mm at baseline to 5.0 ± 1.7 mm at 1 year (p < 0.0001), while PD decreased from 7.8 ± 1.5 mm at baseline to 4.0 ± 0.9 mm at 1 year (p < 0.0001). In the control group, mean CAL changed from 8.6 ± 1.9 mm to 5.9 ± 1.6 mm (p < 0.001) and mean PD improved from 7.4 ± 1.3 mm to 4.1 ± 1.3 mm (p < 0.001). Mean CAL gain measured 3.6 ± 1.6 mm in the test and 2.7 ± 1.6 mm in the control group, respectively. Defect fill (i.e., INTRA DD gain) at re-entry measured 3.7 ± 1.8 mm in the test and 2.7 ± 2.1 mm in the control group. A CAL gain of ≥ 3 mm was measured in 76% of the defects in the test group and in 40% of the defects in the control group, respectively. In both groups, all evaluated clinical and radiographic parameters improved statistically significantly compared with baseline, but no statistically significant differences were found between the two groups. CONCLUSIONS Within their limits, the present study has failed to show any substantial added clinical benefits following the postoperative administration of amoxicillin in conjunction with regenerative periodontal surgery using DBBM and GTR. CLINICAL RELEVANCE The post-surgically administration of systemic antibiotics does not seem to be necessary following regenerative periodontal surgery

Clinical and radiographic evaluation of intrabony periodontal defect treatment by open flap debridement alone or in combination with nanocrystalline hydroxyapatite bone substitute

The aim of this study has been to compare the clinical and radiographic outcome of periodontal intrabony defect treatment by open flap debridement alone or in combination with nanocrystalline hydroxyapatite bone substitute application. Thirty patients diagnosed with advanced periodontits were divided into two groups: the control group (OFD), in which an open flap debridement procedure was performed and the test group (OFD+NHA), in which defects were additionally filled with nanocrystalline hydroxyapatite bone substitute material. Plaque index (PI), gingival index (GI), bleeding on probing (BOP), pocket depth (PD), gingival recession (GR) and clinical attachment level (CAL) were measured prior to, then 6 and 12months following treatment. Radiographic depth and width of defects were also evaluated. There were no differences in any clinical and radiographic parameters between the examined groups prior to treatment. After treatment, BOP, GI, PD, CAL, radiographic depth and width parameter values improved statistically significantly in both groups. The PI value did not change, but the GR value increased significantly after treatment. There were no statistical differences in evaluated parameters between OFD and OFD+NHA groups 6 and 12months after treatment. Within the limits of the study, it can be concluded that the additional use of nanocrystalline hydroxyapatite bone substitute material after open flap procedure does not improve clinical and radiographic treatment outcome

The Cone Beam Computed Tomography Evaluation of Cortical Bone Plate after Piezocision-Assisted Orthodontic Upper Arch Expansion: A Case Series

Background: The purpose of the study was to evaluate cone beam computed tomography (CBCT) after piezocision-assisted orthodontic maxillary arch expansion. Methods: Forty CBCT images of 20 patients taken before and after treatment were included in the study. The following radiographic parameters were measured: buccal/palatal bone plate thickness measured in three locations, 0.5 mm, 3.5 mm, and 5 mm from the margin of alveolar process; cemento-enamel junction-crest distance (CEJ-C) measured at buccal (CEJ-B) and palatal/lingual (CEJ-P) aspects. Results: After treatment there were insignificant changes in CEJ-C and thickness of buccal/palatal plates for all the dental groups except for incisors and premolars. CEJ-B increased by 1.43 mm on premolars and CEJ-P by 1.65 mm on incisors and by 0.31 mm on premolars. On the incisors, the buccal plate width increased significantly, by 0.2 mm and 0.44 mm at 3.5-mm and 5-mm measurement points. On premolars, the buccal plate width decreased in three measuring points by 0.27 mm, 0.37 mm, and 0.25 mm. Conclusions: Piezocision-assisted orthodontic maxillary arch expansion does not cause evident negative changes of cortical plates except for the premolar region. Therefore, premolars may be at greater risk of buccal plate loss than other teeth

A Unique Glassy Cell Carcinoma (GCC) of the Cervix Diagnosed during Pregnancy&mdash;A Case Report

Glassy Cell carcinoma (GCC) of the cervix is classified as a unique, aggressive neoplasm, with different sensitivity to chemotherapy and radiotherapy. It is such an extremely rare tumor that it is practically not observed during pregnancy. Information on the coexistence of cervical GCC with pregnancy is also unique, so it seems extremely important to disseminate it in order to develop the most effective treatment regimen. Additionally, making any decisions regarding therapeutic methods during pregnancy encounters great ethical problems. We present the case of a 26-year-old pregnant woman, 18 weeks gestation, diagnosed with GCC of the cervix, IB3 grade in the International Federation of Gynecology and Obstetrics (FIGO) scale. Despite the unfavorable prognosis, the use of chemotherapy in a pregnant patient brought on a favorable therapeutic effect, without any negative effects on the fetus. The article also presents a literature review on the epidemiology, pathology, immunohistochemistry, treatment and prognosis of this rare disease

The Effect of Nonsurgical Periodontal Therapy on the Level of Human Neutrophil Peptides 1-3 in Patients with Aggressive Periodontitis.

PURPOSE To assess the presence of HNP1-3 in the gingival crevicular fluid (GCF) of patients suffering from aggressive periodontitis before and after nonsurgical periodontal therapy. MATERIALS AND METHODS Twenty patients, each with generalised aggressive periodontitis (GAP) were included in the study. After periodontal examination, one site with a probing depth (PD) ≥ 4 mm was selected. Patients received nonsurgical treatment (scaling and root planing [SRP]) with additional administration of systemic antibiotic therapy (amoxicillin 375 mg three times daily + metronidazole 250 mg three times daily for 7 days). Prior to therapy and 3 and 6 months after, the following parameters were evaluated from the same site: PD, gingival recession (GR), clinical attachment level (CAL), plaque index (PI), bleeding on probing (BOP), sulcus fluid flow rate (SFFR). The level of HNP1-3 in GCF was determined by means of a commercially available ELISA kit. RESULTS Compared to baseline, the level of HNP 1-3 did not show statistically significant differences at 3 and 6 months. The evaluated clinical parameters and SFFR showed statistically significant decreases compared to baseline. At 6 months, PD (median) decreased from 7 to 3.5 and CAL (median) decreased from 7 to 4. CONCLUSION In patients with GAP, nonsurgical periodontal therapy in conjunction with systemic administration of amoxicillin and metronidazole had no effect on the level of HNP1-3 in GCF